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Downregulation of t...
Downregulation of tumor suppressor gene PML in uterine cervical carcinogenesis : impact of human papillomavirus infection (HPV)
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- Singh, Neha (författare)
- Department of Biotechnology, Panjab University, Chandigarh, India / Centre for Stem Cell and Tissue Engineering, Panjab University, Chandigarh, India
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- Sobti, R. C. (författare)
- Department of Biotechnology, Panjab University, Chandigarh, India
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- Suri, Vanita (författare)
- Department of Obstetrics and Gynecology, Post Graduate Institute of Medical Education and Research, Chandigarh, India
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- Nijhawan, Raje (författare)
- Department of Cytology and Gynae Pathology, PGIMER, Sector-12, Chandigarh, India
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- Sharma, Shweta (författare)
- Division of Molecular Genetics and Biochemistry, Institute of Cytology and Preventive Oncology (ICMR), NOIDA, India
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- Das, B. C. (författare)
- Dr. B.R. Ambedkar Research Centre for Biomedical Research (ACBR), University of Delhi (North Campus), Delhi, 110 007, INDIA
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- Bharadwaj, Mausumi (författare)
- Division of Molecular Genetics and Biochemistry, Institute of Cytology and Preventive Oncology (ICMR), NOIDA, India
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- Hussain, Showket (författare)
- Division of Molecular Genetics and Biochemistry, Institute of Cytology and Preventive Oncology (ICMR), NOIDA, India
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(creator_code:org_t)
- Elsevier, 2013
- 2013
- Engelska.
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Ingår i: Gynecologic Oncology. - : Elsevier. - 0090-8258 .- 1095-6859. ; 128:3, s. 420-6
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
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- OBJECTIVES: Cervical cancer is a leading gynecological cancer in Indian women and is caused due to infection with high risk human pappilloma virus (HR-HPV) 16 and 18. It has been well documented that PML (promyelocytic leukemia) enhances viral infectivity and plays a crucial role in antiviral response mechanisms. The aim of the present study was to evaluate the role of PML gene with context to HPV infection in cervical carcinogenesis.METHODS: The expression pattern of PML was analyzed by western blotting and immunohistochemistry in a total of 170 fresh surgically resected cervical tissue specimens comprising precancer (n=12), cancer (n=118) and normal controls (n=40) recruited from PGIMER, Chandigarh, India. HPV status was analyzed by L1 consensus PCR followed by type specific PCR for HR-HPV types 16 and 18 and low risk types 6 and 11.RESULTS: A significant downregulation of PML protein was observed in the majority of cervical cancer and precancer cases 68% (89/130) compared to normal controls. The loss of expression pattern of PML gene was significantly increased with severity of disease both clinically and pathologically (p<0.001). HPV infection was detected in the majority of cancer cases 96% (113/118) and in 83% (10/12) of precancer lesions whereas no infection could be detected in normal controls. Interestingly, all the 68% (89/130) cervical cancer cases that showed downregulation of PML were HPV infected (p=0.0001).CONCLUSION: Taken together, these observations suggest that the downregulation of PML gene and its synergism with HPV infection may play an important role and may serve as a new marker for early diagnosis and therapeutic intervention for cervical carcinogenesis.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Nyckelord
- PML
- Cervical cancer
- HPV
- Naturvetenskap
- Natural sciences
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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