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1.
  • Ankerst, J., et al. (författare)
  • Decreased responsiveness to immune complexes of granulocytes from patients with acute leukemia in remission demonstrated by microcalorimetry
  • 1984
  • Ingår i: Leukemia Research. - : Elsevier BV. - 0145-2126. ; 8:6, s. 997-1002
  • Tidskriftsartikel (refereegranskat)abstract
    • Functional activity of granulocytes from healthy individuals and from patients with acute leukemia in remission was studied. The increase of heat production rate (metabolic activity) after stimulation of the blood cells with in vitro formed immune complexes was measured by microcalorimeters of heat conduction type. It was demonstrated that increased heat production rate after exposure to immune complexes was significantly lower (p<0.0005) in 9 patients with acute leukemia with a remission duration of less than 6 months than in 25 healthy volunteers.
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2.
  • Fäldt, R., et al. (författare)
  • Differentiation of myeloid leukemic cells in vitro demonstrated by microcalorimetry : Stimulation of leukemic and remission cells by IgG-binding Fc receptors
  • 1986
  • Ingår i: Leukemia Research. - : Elsevier BV. - 0145-2126. ; 10:9, s. 1147-1150
  • Tidskriftsartikel (refereegranskat)abstract
    • Interaction of immunoglobulin G (IgG)-coated latex particles with Fc receptors on myeloid leukemic blood cells and on polymorphonuclear granulocytes (PMN) from remission patients and healthy blood donors was investigated using microcalorimetry. The induced heat production by leukemic cells from 13 patients with the M2, M4 and M5 FAB groups (French-American-British classification) of acute myeloid leukemia (AML) was significantly higher than that of leukemic cells from 7 patients with the M1 FAB group (p < 0.005) and mononuclear blood cells from 10 healthy individuals (p < 0.005). The values were similar for PMN from 10 remission patients and 10 healthy blood donors. After incubation of M1 cells in vitro for 24-30 h at 37°C the heat production induced by IgG-coated latex particles by the cells increased significantly, indicating the appearance of Fc receptors for IgG. In addition, the heat production by unstimulated M2, M4 and M5 cells was significantly higher than that by unstimulated M1 cells (p < 0.005) and normal mononuclear cells (p < 0.0005). The heat production by unstimulated PMN suspended in tissue culture medium was similar in remission patients and healthy blood donors.
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