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Träfflista för sökning "L773:0264 6021 ;srt2:(1980-1989);lar1:(lnu)"

Sökning: L773:0264 6021 > (1980-1989) > Linnéuniversitetet

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1.
  • Gierow, Peter, et al. (författare)
  • Heterogeneity of smooth endoplasmic reticulum from rat liver studied by two-phase partitioning
  • 1989
  • Ingår i: Biochemical Journal. - 0264-6021 .- 1470-8728. ; 262:1, s. 55-61
  • Tidskriftsartikel (refereegranskat)abstract
    • Smooth microsomal membranes, prepared from rat liver by sucrose-density-gradient centrifugation, were subfractionated by counter-current distribution in an aqueous two-phase system consisting of poly(ethylene glycol) and Dextran T500. A comparison of the distribution curves of marker enzymes, together with theoretically calculated curves, indicated the presence of at least five membrane subfractions, differing in the ratios of the marker enzymes. Glucose-6-phosphatase and arylesterase distributed in one manner, and NADPH-cytochrome c reductase and NADH-ferricyanide reductase in another. Evidence for further heterogeneities in the distribution of marker enzymes in smooth microsomes was obtained by analysing the membrane domain structure using a recently described method [Albertsson (1988) Q. Rev. Biophys. 21, 61-98]. Phenobarbital treatment did not influence the behaviour of the marker enzymes. 
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2.
  • Gierow, Peter, et al. (författare)
  • Lateral heterogeneity of rat liver plasma membranes analysed by counter-current distribution
  • 1988
  • Ingår i: Biochemical Journal. - 0264-6021 .- 1470-8728. ; 249, s. 369-375
  • Tidskriftsartikel (refereegranskat)abstract
    • The lateral heterogeneity of rat liver plasma membranes was examined by fragmentation and fractionation by counter-current distribution in an aqueous two-phase polymer system. The distribution pattern was analysed by plotting the relative specific activities of marker components against each other. By this analysis asialo-orosomucoid receptors were found in a domain separated from domains containing 5'-nucleotidase and leucine aminopeptidase by another domain devoid of these markers. 5'Nucleotidase and leucine aminopeptidase resided in adjacent but separate domains. The experimental data were compared with corresponding plots of markers in model membranes. The model membranes yielded plots of different shapes depending on marker distribution and fragment size. This method of analysis should be useful for examining the lateral heterogeneity also of other membranes. 
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  • Resultat 1-2 av 2
Typ av publikation
tidskriftsartikel (2)
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refereegranskat (2)
Författare/redaktör
Gierow, Peter (2)
Jergil, B (2)
Albertsson, P-A (1)
Lärosäte
Linnéuniversitetet  (2)Ta bort avgränsningen
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Engelska (2)

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