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- Takahashi, Kayo, et al.
(författare)
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Alteration in [11C]vorozole binding to aromatase in neuronal cells of rat brain induced by anabolic androgenic steroids and flutamide
- 2008
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Ingår i: NeuroReport. - 0959-4965 .- 1473-558X. ; 19:4, s. 431-435
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Tidskriftsartikel (refereegranskat)abstract
- In a previous study, we demonstrated that androgenic-anabolic steroids increased aromatase expression in the bed nucleus of stria terminalis and preoptic area in rat brain, as evaluated using autoradiography with [11C]vorozole, a potential positron emission tomography tracer for aromatase. In this study, we explored whether the increase in aromatase binding is mediated via androgen receptors and whether this increase occurs in neurons or glial cells. Rats were given nandrolone decanoate (15 mg/kg body weight once every 3 days) and flutamide (20 mg/kg/day) alone or in combination for 20 days. Results indicated a significant increase of [11C]vorozole binding by nandrolone decanoate in the bed nucleus of the stria terminalis and preoptic area, as in our previous study. Flutamide treatment, on the other hand, decreased [11C]vorozole binding in the bed nucleus of the stria terminalis, preoptic area, and medial amygdala. Immunohistochemical examination demonstrated that upregulation of aromatase expression occurred in neurons. Our findings suggest that aromatase is regulated through an androgen receptor-mediated system. This aromatase-specific tracer and the positron emission tomography technique could be useful for exploring the role of aromatase in anabolic androgenic steroids abusers.
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| 2. |
- Takahashi, Kayo, et al.
(författare)
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Increase in [11C]vorozole binding to aromatase in the hypothalamus in rats treated with anabolic androgenic steroids
- 2007
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Ingår i: NeuroReport. - 0959-4965 .- 1473-558X. ; 18:2, s. 171-174
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Tidskriftsartikel (refereegranskat)abstract
- In the present study, we investigated the alteration of aromatase expression in the brain by anabolic androgenic steroid treatment in male rats. The rats were given nandrolone decanoate (15 mg/kg/day) for 14 days, and the brains were used for autoradiography with Cvorozole, which has been developed as a positron emission tomography tracer for aromatase by our group. The results indicated a significant increase of Cvorozole binding by anabolic androgenic steroids in the bed nucleus of the stria terminalis and preoptic area. In contrast, no significant change of Cvorozole binding was observed in the medial amygdala. Our results suggest that aromatase is significantly upregulated in the bed nucleus of the stria terminalis and preoptic area by anabolic androgenic steroids and also suggest that androgens regulate aromatase differently in these structures compared with the medial amygdala.
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| 3. |
- Takahashi, Kayo, et al.
(författare)
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Increase in hypothalamic aromatase in macaque monkeys treated with anabolic-androgenic steroids : PET study with [C-11]vorozole
- 2011
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Ingår i: NeuroReport. - 0959-4965 .- 1473-558X. ; 22:7, s. 326-330
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Tidskriftsartikel (refereegranskat)abstract
- In an earlier study in rodents, we showed that the aromatase that converts androgens to estrogens in the preoptic area and bed nucleus of stria terminalis was significantly increased in concentration after exposure to anabolic-androgenic steroids. To confirm whether this occurs in primates, we conducted a positron emission tomographic study using macaque monkeys. Male rhesus monkeys were treated with nandrolone decanoate for 3 weeks. To measure aromatase concentrations, we performed positron emission tomographic imaging using a C-11-labeled specific aromatase inhibitor, [C-11] vorozole. After treatment with nandrolone, significant increase in [C-11] vorozole binding was observed in the hypothalamus but not other areas including the amygdala, which is also aromatase enriched. These findings in monkeys are consistent with those we obtained earlier in rats. These findings strongly suggest that aromatase in the hypothalamus may play a crucial role in the emotional instability of anabolic-androgenic steroids abusers. NeuroReport 22:326-330 (C) 2011 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.
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