| 1. |
- Deschout, Hendrik, et al.
(författare)
-
Straightforward FRAP for quantitative diffusion measurements with a laser scanning microscope
- 2010
-
Ingår i: Optics Express. - 1094-4087. ; 18:22, s. 22886-22905
-
Tidskriftsartikel (refereegranskat)abstract
- Confocal or multi-photon laser scanning microscopes are convenient tools to perform FRAP diffusion measurements. Despite its popularity, accurate FRAP remains often challenging since current methods are either limited to relatively large bleach regions or can be complicated for non-specialists. In order to bring reliable quantitative FRAP measurements to the broad community of laser scanning microscopy users, here we have revised FRAP theory and present a new pixel based FRAP method relying on the photo bleaching of rectangular regions of any size and aspect ratio. The method allows for fast and straightforward quantitative diffusion measurements due to a closed–form expression for the recovery process utilizing all available spatial and temporal data. After a detailed validation, its versatility is demonstrated by diffusion studies in heterogeneous biopolymer mixtures.
|
|
| 2. |
- Thorin, Emil, et al.
(författare)
-
Simultaneous detection of K, KOH, and KCl in flames and released from biomass using photofragmentation TDLAS
- 2021
-
Ingår i: Optics Express. - : The Optical Society. - 1094-4087. ; 29:26
-
Tidskriftsartikel (refereegranskat)abstract
- Gaseous potassium (K) species released from biomass during thermochemicalconversion pose challenges to reactors and human health. Photofragmentation tunable diodelaser absorption spectroscopy (PF-TDLAS) was used for simultaneous, high-dynamic rangemeasurements of atomic K, potassium hydroxide (KOH) and potassium chloride (KCl) inflat flames seeded with KCl salt. An expression for the PF-TDLAS signal is presented andexperimentally verified. Axial K species concentration profiles recorded at fuel-air equivalenceratios of 0.8 and 1.3 are compared to 2D axisymmetric reacting flow simulations. An overallgood agreement is found, but KOH is over-predicted in simulations of fuel-rich flames at theexpense of atomic K. Quantification of K species close to softwood and wheat straw particlesconverted in the flames is demonstrated.
|
|
