Sökning: L773:1363 9811 OR L773:1469 8382
> Mallmin Hans >
Global miRNA expres...
Global miRNA expression and correlation with mRNA levels in primary human bone cells
-
- Laxman, Navya (författare)
- Uppsala universitet,Endokrinologi och mineralmetabolism,Science for Life Laboratory, SciLifeLab
-
- Rubin, Carl-Johan (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
-
- Mallmin, Hans (författare)
- Uppsala universitet,Ortopedi
-
visa fler...
-
- Nilsson, Olle (författare)
- Uppsala universitet,Ortopedi
-
Pastinen, Tomi (författare)
-
Grundberg, Elin (författare)
-
- Kindmark, Andreas (författare)
- Uppsala universitet,Endokrinologi och mineralmetabolism,Science for Life Laboratory, SciLifeLab
-
visa färre...
-
(creator_code:org_t)
- 2015-06-15
- 2015
- Engelska.
-
Ingår i: RNA. - : Cold Spring Harbor Laboratory. - 1355-8382 .- 1469-9001. ; 21:8, s. 1433-1443
- Relaterad länk:
-
https://doi.org/10.1...
-
visa fler...
-
https://uu.diva-port... (primary) (Raw object)
-
http://rnajournal.cs...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- MicroRNAs (miRNAs) are important post-transcriptional regulators that have recently introduced an additional level of intricacy to our understanding of gene regulation. The aim of this study was to investigate miRNA-mRNA interactions that may be relevant for bone metabolism by assessing correlations and interindividual variability in miRNA levels as well as global correlations between miRNA and mRNA levels in a large cohort of primary human osteoblasts (HOBs) obtained during orthopedic surgery in otherwise healthy individuals. We identified differential expression (DE) of 24 miRNAs, and found 9 miRNAs exhibiting DE between males and females. We identified hsa-miR-29b, hsa-miR-30c2, and hsa-miR-125b and their target genes as important modulators of bone metabolism. Further, we used an integrated analysis of global miRNA-mRNA correlations, mRNA-expression profiling, DE, bioinformatics analysis, and functional studies to identify novel target genes for miRNAs with the potential to regulate osteoblast differentiation and extracellular matrix production. Functional studies by overexpression and knockdown of miRNAs showed that, the differentially expressed miRNAs hsa-miR-29b, hsa-miR-30c2, and hsa-miR-125b target genes highly relevant to bone metabolism, e.g., collagen, type I, alpha 1 (COL1A1), osteonectin (SPARC), Runt-related transcription factor 2 (RUNX2), osteocalcin (BGLAP), and frizzled-related protein (FRZB). These miRNAs orchestrate the activities of key regulators of osteoblast differentiation and extracellular matrix proteins by their convergent action on target genes and pathways to control the skeletal gene expression.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Nyckelord
- miRNA
- mRNA
- osteoblasts
- interindividual variation
- differential expression
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
-
RNA
(Sök värdpublikationen i LIBRIS)
Till lärosätets databas