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Träfflista för sökning "L773:1472 6831 ;conttype:(refereed);spr:eng;pers:(Carlén Anette 1950)"

Sökning: L773:1472 6831 > Refereegranskat > Engelska > Carlén Anette 1950

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1.
  • Mannaa, Alaa, et al. (författare)
  • Supragingival plaque microbial analysis in reflection to caries experience.
  • 2013
  • Ingår i: BMC oral health. - : Springer Science and Business Media LLC. - 1472-6831. ; 13
  • Tidskriftsartikel (refereegranskat)abstract
    • ABSTRACT: BACKGROUND: Dental caries develops as a result of the metabolism of carbohydrates by cariogenic bacteria present in a complex biofilm. The present study aimed to examine if bacteria in pooled supragingival plaque samples quantified using a "checkerboard DNA-DNA hybridization" based panel of caries-related bacteria, could reflect the caries experience in a manner similar to saliva samples analysed using a chair-side method in a previous investigation. METHODS: A total of 86 mothers and their children aged 4-6 years and 12-16 years old participated. Caries experience (DMFT/dmft; Decayed, Missing and Filled Teeth for permanent and primary teeth) was registered clinically and radiographically. Caries was recorded at the D3 level (caries into dentine). The D/d component was divided into three categories. A pooled supragingival plaque sample per participant was obtained from posterior approximal sites. Analyses of 15 bacterial species were performed using the checkerboard DNA-DNA hybridisation technique. RESULTS: No significant relationships were found between the bacterial scores and DMFT/dmft nor D/d groups. CONCLUSIONS: Unlike the saliva samples and the chair-side method, interproximal pooled plaque samples analysed using the "checkerboard DNA-DNA hybridization technique" did not reveal any significant relations between the bacterial counts and the caries experience.
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2.
  • Dahlén, Gunnar, 1944, et al. (författare)
  • Rapid urease test (RUT) for evaluation of urease activity in oral bacteria in vitro and in supragingival dental plaque ex vivo
  • 2018
  • Ingår i: BMC Oral Health. - : Springer Science and Business Media LLC. - 1472-6831. ; 18:1
  • Tidskriftsartikel (refereegranskat)abstract
    • Background: Urease is an enzyme produced by plaque bacteria hydrolysing urea from saliva and gingival exudate into ammonia in order to regulate the pH in the dental biofilm. The aim of this study was to assess the urease activity among oral bacterial species by using the rapid urease test (RUT) in a micro-plate format and to examine whether this test could be used for measuring the urease activity in site-specific supragingival dental plaque samples ex vivo. Methods: The RUT test is based on 2% urea in peptone broth solution and with phenol red at pH 6.0. Oral bacterial species were tested for their urease activity using 100 μl of RUT test solution in the well of a micro-plate to which a 1 μl amount of cells collected after growth on blood agar plates or in broth, were added. The color change was determined after 15, 30 min, and 1 and 2 h. The reaction was graded in a 4-graded scale (none, weak, medium, strong). Ex vivo evaluation of dental plaque urease activity was tested in supragingival 1 μl plaque samples collected from 4 interproximal sites of front teeth and molars in 18 adult volunteers. The color reaction was read after 1 h in room temperature and scored as in the in vitro test. Results: The strongest activity was registered for Staphylococcus epidermidis, Helicobacter pylori, Campylobacter ureolyticus and some strains of Haemophilus parainfluenzae, while known ureolytic species such as Streptococcus salivarius and Actinomyces naeslundii showed a weaker, variable and strain-dependent activity. Temperature had minor influence on the RUT reaction. The interproximal supragingival dental plaque between the lower central incisors (site 31/41) showed significantly higher scores compared to between the upper central incisors (site 11/21), between the upper left first molar and second premolar (site 26/25) and between the lower right second premolar and molar (site 45/46). Conclusion: The rapid urease test (RUT) in a micro-plate format can be used as a simple and rapid method to test urease activity in bacterial strains in vitro and as a chair-side method for testing urease activity in site-specific supragingival plaque samples ex vivo. © 2018 The Author(s).
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Lingström, Peter, 19 ... (1)
Dahlén, Gunnar, 1944 (1)
Campus, Guglielmo (1)
Hassan, Haidar (1)
Blomqvist, Susanne (1)
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Mannaa, Alaa (1)
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Göteborgs universitet (2)
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Medicin och hälsovetenskap (2)

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