| 1. |
- Belfrage, Per, et al.
(författare)
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Methods for the determination in the nanomole range of lipids in liver fine-needle aspiration biopsies
- 1970
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Ingår i: Scandinavian Journal of Clinical & Laboratory Investigation. - : Informa UK Limited. - 1502-7686 .- 0036-5513. ; 26:1, s. 53-60
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Tidskriftsartikel (refereegranskat)abstract
- Triglycerides and phospholipids 0.5-2mg (wet weight) of human liver material obtained by the fine-needle aspiration biopsy technique can be estimated after chloroform:methanol extraction by the determination of lipid phosphorus and glyceride glycerol. The methods are based on the colorimetric determination of inorganic phosphate after oxidation of the phospholipids and the enzymatic fluorometric determination of glycerol after complete hydrolysis of the triglycerides, catalyzed by purified pancreatic lipase. The accumulation of lipids is then expressed as the increase in the molar ratio of triglycerides over phospholipids.
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| 2. |
- Wiebe, Thomas, et al.
(författare)
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Human liver lipids: representativity of determinations on material obtained by fine-needle aspiration biopsy
- 1971
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Ingår i: Scandinavian Journal of Clinical & Laboratory Investigation. - : Informa UK Limited. - 1502-7686 .- 0036-5513. ; 28:4, s. 453-457
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Tidskriftsartikel (refereegranskat)abstract
- Triglycerides and phospholipids in human liver material obtained by fine-needle aspiration biopsy have been determined according to an improved version of a previously developed method. The molar ratios of these lipids have been compared with the same ratios determined on material representing whole liver. The average value of the ratios in the fine-needle specimens was 101% (83-113%) of the corresponding whole-liver value. The variation coefficient of determinations on several fine-needle specimens from the same liver was 10% (4-17%). The least difference, between the ratiosof two consecutive fine-needle specimens, to ensure a significant difference was 30%.
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| 3. |
- Wiebe, Thomas, et al.
(författare)
-
Time-course of liver fat accumulation in man after a single load of ethanol
- 1971
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Ingår i: Scandinavian Journal of Clinical & Laboratory Investigation. - : Informa UK Limited. - 1502-7686 .- 0036-5513. ; 27:1, s. 33-36
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Tidskriftsartikel (refereegranskat)abstract
- Three healthy young male subjects were given a single load of ethanol (approximately 3 g/kg body weight) over 5 hours. Liver aspiration biopsies were performed at the start of the experiment and 6, 14, 24, and 48 hours after the start. Blood alcohol and serum transaminase activity levels were measured simultaneously. Triglycerides and phospholipids of the liver biopsy material were determined by a previously described method. The increase of the molar ratio triglycerides over phospholipids (TG/PL) was used to express triglyceride accumulation in the livers. At 24 hours from the start of the experiment this ratio had increased 2-5 times, and then declined to almost the initial values at 48 hours. Serum transaminases showed a slight increase over the entire experimental period. Considerable individual variation in the parameters was found. The results show that it is possible to follow time-course of rapid metabolic changes in the human liver by a combination of the fine-needle aspiration biopsy technique and biochemical micro-methods.
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