SwePub
Sök i LIBRIS databas

  Extended search

L773:1559 1174
 

Search: L773:1559 1174 > Bergquist Jonas > Scavenger Receptor ...

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Scavenger Receptor A Mediates the Clearance and Immunological Screening of MDA-Modified Antigen by M2-Type Macrophages

Warnecke, Andreas (author)
Karolinska Inst, Appl Immunol & Immunotherapy, Dept Clin Neurosci, Ctr Mol Med,Karolinska Univ Hosp Solna, S-17176 Solna, Sweden.
Abele, Sonja (author)
Karolinska Inst, Appl Immunol & Immunotherapy, Dept Clin Neurosci, Ctr Mol Med,Karolinska Univ Hosp Solna, S-17176 Solna, Sweden.
Musunuri, Sravani (author)
Uppsala universitet,Analytisk kemi,Science for Life Laboratory, SciLifeLab
show more...
Bergquist, Jonas (author)
Uppsala universitet,Analytisk kemi,Science for Life Laboratory, SciLifeLab
Harris, Robert A. (author)
Karolinska Institutet
show less...
Karolinska Inst, Appl Immunol & Immunotherapy, Dept Clin Neurosci, Ctr Mol Med,Karolinska Univ Hosp Solna, S-17176 Solna, Sweden Analytisk kemi (creator_code:org_t)
2017-08-21
2017
English.
In: Neuromolecular medicine. - : Springer Science and Business Media LLC. - 1535-1084 .- 1559-1174. ; 19:4, s. 463-479
  • Journal article (peer-reviewed)
Abstract Subject headings
Close  
  • In this study, we investigated the uptake of malondialdehyde (MDA)-modified myelin oligodendrocyte glycoprotein (MOG) in the context of lipid peroxidation and its implications in CNS autoimmunity. The use of custom-produced fluorescently labeled versions of MOG or MDA-modified MOG enabled us to study and quantify the uptake by different macrophage populations and to identify the responsible receptor, namely SRA. The SRA-mediated uptake of MDA-modified MOG is roughly tenfold more efficient compared to that of the native form. Notably, this uptake is most strongly associated with anti-inflammatory M2-type macrophages. MDA-modified MOG was demonstrated to be resistant to degradation by lysine-dependent proteases in vitro, but the overall digestion fragments appeared to be similar in cell lysates, although their relative abundance appeared to be altered as a result of faster uptake. Accordingly, MDA-modified MOG is processed for presentation by APCs, allowing maximized recall proliferation of MOG(35-55)-specific 2D2 T cells in vitro due to higher uptake. However, MDA modification of MOG did not enhance immune priming or disease course in the in vivo MOG-EAE model, but did induce antibody responses to both MOG and MDA adducts. Taken together our results indicate that MDA adducts primarily constitute clearance signals for phagocytes and promote rapid removal of antigen, which is subjected to immunological screening by previously licensed T cells.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Neurologi (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Neurology (hsv//eng)

Keyword

Malondialdeyde
Macrophages
MOG-EAE
Autoimmunity
Scavenger receptors
Posttranslational modifications

Publication and Content Type

ref (subject category)
art (subject category)

Find in a library

To the university's database

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Search outside SwePub

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view