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Träfflista för sökning "L773:1613 6810 OR L773:1613 6829 ;pers:(Liedberg Bo)"

Sökning: L773:1613 6810 OR L773:1613 6829 > Liedberg Bo

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1.
  • Aili, Daniel, 1977-, et al. (författare)
  • Colorimetric Protein Sensing by Controlled Assembly of Gold Nanoparticles Functionalized with Synthetic Receptors
  • 2009
  • Ingår i: Small. - : Wiley. - 1613-6810 .- 1613-6829. ; 5:21, s. 2445-2452
  • Tidskriftsartikel (refereegranskat)abstract
    • A novel strategy is described for the colorimetric sensing of proteins, based on polypeptide-functionalized gold nanoparticles. Recognition is accomplished using a polypeptide sensor scaffold designed to specifically bind to the model analyte, human carbonic anhydrase II (HCAII). The extent of particle aggregation, induced by the Zn2+-triggered dimerization and folding of a second polypeptide also present on the surface of the gold nanoparticle, gives a readily detectable colorimetric shift that is dependent on the concentration of the target protein. In the absence of HCAII, particle aggregation results in a major redshift of the plasmon peak, whereas analyte binding prevented the formation of dense aggregates, significantly reducing the magnitude of the redshift. The versatility of the technique is demonstrated using a second model system based on the recognition of a peptide sequence from the tobacco mosaic virus coat protein (TMVP) by a recombinant antibody fragment (Fab57P). Concentrations down to approximate to 10 nM and approximate to 25 nM are detected for HCAII and Fab57P, respectively. This strategy is proposed as a generic platform for robust and specific protein analysis that can be further developed to monitor a wide range of target proteins.
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2.
  • Aili, Daniel, et al. (författare)
  • Colorimetric sensing: Small 21/2009
  • 2009
  • Ingår i: Small. - : John Wiley & Sons. - 1613-6810 .- 1613-6829. ; 5:21
  • Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
    • The cover picture illustrates a novel concept for colorimetric protein sensing based on the controllable assembly of polypeptide-functionalized gold nanoparticles. Recognition of the analyte is accomplished by polypeptide-based synthetic receptors immobilized on gold nanoparticles. Also present on the particle surface is a de novo-designed helix-loop-helix polypeptide that homodimerizes and folds into four-helix bundles in the presence of Zn2+, resulting in particle aggregation. Analyte binding interferes with the folding-induced aggregation, giving rise to a clearly detectable colorimetric response.
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