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Träfflista för sökning "L773:2000 8686 OR L773:2000 8686 ;pers:(González Acuña Daniel)"

Sökning: L773:2000 8686 OR L773:2000 8686 > González Acuña Daniel

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1.
  • Eriksson, Per, et al. (författare)
  • Evaluation and optimization of microbial DNA extraction from fecal samples of wild Antarctic bird species
  • 2017
  • Ingår i: Infection Ecology & Epidemiology. - : Informa UK Limited. - 2000-8686. ; 7:1
  • Tidskriftsartikel (refereegranskat)abstract
    • Introduction: Advances in the development of nucleic acid-based methods have dramatically facilitated studies of host-microbial interactions. Fecal DNA analysis can provide information about the host's microbiota and gastrointestinal pathogen burden. Numerous studies have been conducted in mammals, yet birds are less well studied. Avian fecal DNA extraction has proved challenging, partly due to the mixture of fecal and urinary excretions and the deficiency of optimized protocols. This study presents an evaluation of the performance in avian fecal DNA extraction of six commercial kits from different bird species, focusing on penguins.Material and methods: Six DNA extraction kits were first tested according to the manufacturers' instructions using mallard feces. The kit giving the highest DNA yield was selected for further optimization and evaluation using Antarctic bird feces.Results: Penguin feces constitute a challenging sample type: most of the DNA extraction kits failed to yield acceptable amounts of DNA. The QIAamp cador Pathogen kit (Qiagen) performed the best in the initial investigation. Further optimization of the protocol resulted in good yields of high-quality DNA from seven bird species of different avian orders.Conclusion: This study presents an optimized approach to DNA extraction from challenging avian fecal samples.
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2.
  • Wallensten, Anders, et al. (författare)
  • Extended spectrum beta-lactamases detected in Escherichia coli from gulls in Stockholm, Sweden
  • 2011
  • Ingår i: Infection Ecology & Epidemiology. - : Informa UK Limited. - 2000-8686. ; 1, s. 7030-
  • Tidskriftsartikel (refereegranskat)abstract
    • In order to investigate if bacterial antibiotic resistance was present in gull populations in urbanised areas, we conducted a study in which faecal samples from gulls were collected in central Stockholm, Sweden in April and May 2010 and screened for extended spectrum beta-lactamases (ESBL)-type antibiotic resistance. Eighteen of 194 randomly selected Escherichia coli isolates harboured ESBL of CTX-M phenotype. Since the bacteria are unlikely to have developed the resistance in gulls, it may indicate leakage of resistant bacteria to the environment. As many gulls find food and shelter in cities around the world and thereby share their habitat with dense human populations, the finding that as many as 9% of gulls carry ESBL-type antibiotic resistance may imply that zoonotic transmission between gulls, humans, and other animals is likely to occur in such places. This study illustrates how ecologically widespread the problem of antibiotic resistance has become and this has implications for future policy making to reduce the spread of bacteria with antibiotic resistance.
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  • Resultat 1-2 av 2
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refereegranskat (2)
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Olsen, Björn (2)
González-Acuña, ... (2)Ta bort avgränsningen
Ellström, Patrik (1)
Hernandez, Jorge (1)
Eriksson, Per (1)
Mourkas, Evangelos (1)
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Wallensten, Anders (1)
Drobni, Mirva (1)
Ardiles, Karen (1)
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