| 1. |
- Augustsson, P., et al.
(författare)
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Acoustop horesis pre-alignment of cells enables labelfree enrichment of prostate cancer cells in blood
- 2012
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Ingår i: Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. - 9780979806452 ; , s. 1696-1698
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Konferensbidrag (refereegranskat)abstract
- We have developed a microfluidic chip for isolation of circulating tumor cells (CTCs) in blood based on microchannel acoustophoresis. Ultrasound radiation forces are used to separate cancer cells from blood cells in a continuous flow format. Separation was dramatically improved after incorporation of a 2-dimensional acoustic pre-alignment of the cells before entering the acoustophoresis separation channel. Temperature regulation of the microfluidic chip (±0.5?C) enabled stable and reproducible separation. The chip constitutes a further development and refinement of methods previously presented by our group at μTAS [1-3].
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| 2. |
- Ohlander, A., et al.
(författare)
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DNA melting curve analysis on semi-transparent thin film microheater on flexible lab-on-foil substrate
- 2012
-
Ingår i: Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. - 9780979806452 ; , s. 797-799
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Konferensbidrag (refereegranskat)abstract
- This paper presents genotyping on a novel microheater concept using semi-transparent copper microheaters manufactured by roll-to-roll and lift-off on polyethylene napthalate (PEN) foil. Using a mesh structure, heater surfaces have been realized in one single metallization step with a manufacturing robustness higher than conventional meander structures. The thermal distribution of the meshes, evaluated using thermochromic-liquid-crystals (TLC), produced more homogenous heating characteristics compared to meanders. Parylene coated heaters were functionalized using copolymer poly(DMA-NAS-MAPS) to enable covalent DNA immobilization and successful melting curve analysis was performed differentiating between match and mismatch oligonucleotides.
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| 3. |
- Ghasemi, Masoomeh, et al.
(författare)
-
Separation of deformable hydrogel microparticles in deterministic lateral displacement devices
- 2012
-
Ingår i: Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. - 9780979806452 ; , s. 1672-1674
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Konferensbidrag (refereegranskat)abstract
- To better understand how deformable and non-spherical particles behave in sorting devices based on deterministic lateral displacement we generate models of biological particles with tunable size, shape and mechanical properties using stop-flow lithography and we explore how these parameters play a role in our separation devices. Hollow and solid cylinders are compared with respect to their deformability and their overall behavior in the device. Future work will expand the approach to a range of particle shapes and to particles with varied hydrogel composition to independently control the mechanical properties of the material.
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