1. |
- Ohlander, A., et al.
(författare)
-
DNA melting curve analysis on semi-transparent thin film microheater on flexible lab-on-foil substrate
- 2012
-
Ingår i: Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. - 9780979806452 ; , s. 797-799
-
Konferensbidrag (refereegranskat)abstract
- This paper presents genotyping on a novel microheater concept using semi-transparent copper microheaters manufactured by roll-to-roll and lift-off on polyethylene napthalate (PEN) foil. Using a mesh structure, heater surfaces have been realized in one single metallization step with a manufacturing robustness higher than conventional meander structures. The thermal distribution of the meshes, evaluated using thermochromic-liquid-crystals (TLC), produced more homogenous heating characteristics compared to meanders. Parylene coated heaters were functionalized using copolymer poly(DMA-NAS-MAPS) to enable covalent DNA immobilization and successful melting curve analysis was performed differentiating between match and mismatch oligonucleotides.
|
|