Development of a microfluidic concentration gradient generator on a microwell slide for high-throughput cell analysis

• The present study reports a newly developed, microfluidic concentration gradient generator that adds substantial utility to the high-throughput nature of the microwell slide that we have previously established [1, 2]. Using microfluidic dynamics, we have designed microchannels to deliver a reagent and its dilutant with precisely controlled flow volumes, generating 8 discrete steps of reagent concentration in designated microwells. As proof of concept FITC-labeled dextran and dextran-free water was used to visualize the concentration gradient. The next step will be to perform cancer drug concentration gradient experiments on an animal cell line.

Ämnesord

NATURVETENSKAP  -- Biologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences (hsv//eng)

Nyckelord

Cell analysis

High-throughput

Microfluidic concentration gradient

Microwells

Publikations- och innehållstyp

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