| 1. |
- Edlund, T, et al.
(författare)
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Isolation of cDNA sequences coding for a part of human tissue plasminogen activator.
- 1983
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Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - 0027-8424 .- 1091-6490. ; 80:2, s. 349-52
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Tidskriftsartikel (refereegranskat)abstract
- We have isolated a cDNA sequence coding for a part of human tissue plasminogen activator. mRNA coding for tissue plasminogen activator was partially purified, copied into double-stranded cDNA, and cloned into Escherichia coli. Two sets of partially overlapping oligodeoxynucleotide mixtures corresponding to all possible coding sequences for a known portion of the tissue plasminogen activator gene were prepared. One set was used as a probe to screen cDNA containing bacterial clones and both were used as probes in hybridization against purified plasmid DNA. Of 4,200 bacterial clones examined, 1 carried a plasmid that hybridized to both sets of oligonucleotides. This plasmid contained a 370-base-pair cDNA insert, which was shown by nucleotide sequence analysis to code for the cleavage site region in the one-chain form of the human tissue plasminogen activator.
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| 2. |
- Engquist, Björn, et al.
(författare)
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One-sided difference schemes and transonic flow
- 1980
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Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 0027-8424 .- 1091-6490. ; 77:6, s. 3071-3074
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Tidskriftsartikel (refereegranskat)abstract
- Two one-sided conservation form difference approximations to a scalar one-dimensional convex conservation law are introduced. These are respectively of first- and second-order accuracy and each has the minimum possible band-width. They are nonlinearly stable, they converge only to solutions satisfying the entropy condition, and they have sharp monotone profiles. No such stable approximation of order higher than two is possible. Dimensional splitting algorithms are constructed and used to approximate the small-disturbance equation of transonic flow. These approximations are also nonlinearly stable and without nonphysical limit solutions.
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| 3. |
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| 4. |
- Lund, B, et al.
(författare)
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Novel cluster of alpha-interferon gene sequences in a placental cosmid DNA library.
- 1984
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Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - 0027-8424 .- 1091-6490. ; 81:8, s. 2435-9
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Tidskriftsartikel (refereegranskat)abstract
- A human cosmid library was constructed and probed with a human alpha interferon (IFN-alpha) cDNA clone. One clone giving a strong hybridizing signal was isolated and characterized. The cosmid DNA insert represents a section of the human genome containing three regions of IFN-alpha-like sequences. The DNA was characterized with restriction endonuclease mapping, thereby allowing comparison to similar linkage groups reported recently and determination of homologous regions on the known physical map. The three IFN-alpha-like sequences were analyzed by a partial sequence analysis. Mapping and sequence data establish this section as a not-yet-described cluster of IFN-alpha sequences in the human genome; however, a part of the section matches to some degree to a previously described genomic region. The region described here could represent genetic polymorphism or a duplicated segment.
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| 5. |
- Ny, Tor, et al.
(författare)
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The structure of the human tissue-type plasminogen activator gene : correlation of intron and exon structures to functional and structural domains.
- 1984
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Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - 0027-8424 .- 1091-6490. ; 81:17, s. 5355-9
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Tidskriftsartikel (refereegranskat)abstract
- A genomic clone carrying the human tissue-type plasminogen activator (t-PA) gene was isolated from a cosmid library, and the gene structure was elucidated by restriction mapping, Southern blotting, and DNA sequencing. The cosmid contained all the coding parts of the mRNA, except for the first 58 bases in the 5' end of the mRNA, and had a total length of greater than 20 kilobases. It was separated into at least 14 exons by at least 13 introns, and the exons seemed to code for structural or functional domains. Thus, the signal peptide, the propeptide, and the domains of the heavy chain, including the regions homologous to growth factors, and to the "finger" structure of fibronectin, are all encoded by separate exons. In addition, the two kringle regions of t-PA were both coded for by two exons and were cleaved by introns at identical positions. The region coding for the light chain, comprising the serine protease part of the molecule was split by four introns, revealing a gene organization similar to other serine proteases.
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