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| 35. |
- Rydén, Lisa, et al.
(författare)
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Decreased angiogenic activity in breast cancer in ever-users of oral contraceptive therapy--preliminary report
- 2003
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Ingår i: Anticancer research. - 1791-7530. ; 23:3C, s. 2875-2878
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Angiogenic activity defined by microvessel density or measurement of vascular endothelial growth factor is a key process under physiological and malignant conditions in steroid hormone responding organs. The aim of this study was to relate microvessel density (MVD) in primary breast cancer to reproductive data and use of exogenous hormones. MATERIAL AND METHODS: MVD was calculated retrospectively in forty-two consecutive tumours and related to clinical, histopathological and gynecological data. RESULTS: Tumours in ever-users of oral contraceptive therapy (OC) had lower MVD (p = 0.002), a finding not explained by smaller tumour size or lower histological grade. There was no influence on MVD by other reproductive data. CONCLUSION: These preliminary data on a supposed interaction between the use of OC and angiogenesis in breast cancer indicate that biological properties in breast tumours may be altered by ever-use of OC, but have to be further explored in an extended number of patients.
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| 36. |
- Sandgren, Staffan, et al.
(författare)
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Suramin selectively inhibits carcinoma cell growth that is dependent on extracellular polyamines.
- 2003
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Ingår i: Anticancer research. - 1791-7530. ; 23:2B, s. 1223-1228
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Tidskriftsartikel (refereegranskat)abstract
- Polyamines are necessary for tumour cell growth. Inhibition of endogenous polyamine biosynthesis results in compensatory up-regulation of polyamine uptake. Here, the combined effect of suramin and the polyamine biosynthesis inhibitor alpha-difluoromethylornithine (DFMO) on human carcinoma cell proliferation was studied. Suramin selectively inhibited the growth of tumour cells made dependent on extracellular polyamines by DFMO-treatment. In an animal tumour model, low non-toxic doses of suramin resulted in a 2-fold increase in DFMO tumour growth reduction. Moreover, suramin bound strongly to polyamine-agarose and significantly inhibited polyamine uptake in DFMO-treated cells. Our results indicate that non-toxic doses of suramin augment tumour growth inhibition by DFMO, and that a combination of these well-studied anticancer drugs may represent an additional strategy for cancer treatment.
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| 37. |
- Seth, A, et al.
(författare)
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Gene expression profiling of ductal carcinomas in situ and invasive breast tumors
- 2003
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Ingår i: Anticancer research. - 1791-7530. ; 23:3A, s. 2043-2051
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Tidskriftsartikel (refereegranskat)abstract
- Comparative and functional genomics are powerful tools to advance the understanding of the molecular basis of cancer. It is believed that genes are epigenetically regulated and, thus, each tumor type and stage will be characterized by a gene expression fingerprint. In this study we identified genes that are differentially expressed in ductal carcinoma in situ and invasive ductal carcinoma of the breast. To isolate genes that are associated with progression of breast cancer we performed differential display and subtractive cloning procedures using matched RNA from normal and tumor tissue. cDNA microarray analysis generated gene expression profiles typical of the transition front in situ to invasive breast cancer when we used mRAA extracted from a case of low-to intermediate-grade DCIS and a case of high-grade DC1S/IDC. cDNAs from these samples were the probes in a cDNA microarray hybridization to 9183 unique cDAAs representing 8507 genes. Signals from both transcriptomes were obtained for 8083 genes, and the balanced differential expression values between pure DCIS and DCIS/invasive tumors revealed 303 distinct cDNAs with a ratio of > 2. Interferon inducible genes were found to be expressed at the highest level in the pure DCIS sample. Genes most abundantly expressed in the invasive tumor were immunoglobulin heavy constant gamma 3 and calgranulin B. Further analysis of RNA and protein expression in breast tumor cell lines and patient tissue samples revealed that: IGFBP-rP1 is down-regulated in invasive tumors whereas cyclin I protein is regulated by ubiquitination and is associated with ER-negative breast cancers. Conclusion: The known and novel genes discussed here represent targets for molecular characterization during breast cancer development as well as,for designing novel strategies for diagnosis and treatment.
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