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- Bodin, Aase Katarina, 1977, et al.
(författare)
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CELL 11-Cells like cellulose scaffolds
- 2008
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Ingår i: Abstracts of Papers of the American Chemical Society. - 0065-7727. ; 235:APR 6
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)
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| 3. |
- Bodin, Aase Katarina, 1977, et al.
(författare)
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Manufacturing and characterization of bacterial cellulose tubes using two different fermentation techniques
- 2006
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Ingår i: In: Modern multidisciplinary applied lmicrobiology; Exploiting microbes and their interactions/Ed A Medez-Vilas. - Weinheim, Germany : Wiley-VCH Verlag GmbH & Co. KGaA. - 9783527316113 ; , s. 619-622
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- A modified defined media with 0.6% lactate resulted in high cellulose yield and was therefore used in the tube formation. Two fermentation techniques were compared, A and B, using a modified disc reactor. Scanning Electron Microscopy (SEM) showed that tubes from method A were more homogeneous with denser parts in the network. In contrast, the tubes grown according to method B have denser inner surface and a porous outer surface. Mechanical measurements could not be done on the tubes from method A due to their fragility. However the tubes from method B were measured and showed to be stiffer, stronger, and less extensible when compared with a BC pellicle. This can probably be explained by a slightly higher solid content of the tubes and presumably a more interconnected fibril network as a consequence. The BC tubes manufactured according to fermentation method B results in desired asymmetric structure and mechanical properties and are expected to be used as a scaffold for tissue engineered blood vessels.
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| 7. |
- Concaro, Sebastian, et al.
(författare)
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Bioreactors for Tissue Engineering of Cartilage
- 2009
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Ingår i: Advances in Biochemical Engineering/Biotechnology. - 0724-6145 .- 1616-8542. - 9783540693574 ; 112, s. 125-143
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- The cartilage regenerative medicine field has evolved during the last decades. The first-generation technology, autologous chondrocyte transplantation (ACT) involved the transplantation of in vitro expanded chondrocytes to cartilage defects. The second generation involves the seeding of chondrocytes in a three-dimensional scaffold. The technique has several potential advantages such as the ability of arthroscopic implantation, in vitro pre-differentiation of cells and implant stability among others (Brittberg M, Lindahl A, Nilsson A, Ohlsson C, Isaksson O, Peterson L, N Engl J Med 331(14):889-895, 1994; Henderson I, Francisco R, Oakes B, Cameron J, Knee 12(3):209-216, 2005; Peterson L, Minas T, Brittberg M, Nilsson A, Sjogren-Jansson E, Lindahl A, Clin Orthop (374):212-234, 2000; Nagel-Heyer S, Goepfert C, Feyerabend F, Petersen JP, Adamietz P, Meenen NM, et al. Bioprocess Biosyst Eng 27(4):273-280, 2005; Portner R, Nagel-Heyer S, Goepfert C, Adamietz P, Meenen NM, J Biosci Bioeng 100(3):235-245, 2005; Nagel-Heyer S, Goepfert C, Adamietz P, Meenen NM, Portner R, J Biotechnol 121(4):486-497, 2006; Heyland J, Wiegandt K, Goepfert C, Nagel-Heyer S, Ilinich E, Schumacher U, et al. Biotechnol Lett 28(20):1641-1648, 2006). The nutritional requirements of cells that are synthesizing extra-cellular matrix increase along the differentiation process. The mass transfer must be increased according to the tissue properties. Bioreactors represent an attractive tool to accelerate the biochemical and mechanical properties of the engineered tissues providing adequate mass transfer and physical stimuli. Different reactor systems have been [5] developed during the last decades based on different physical stimulation concepts. Static and dynamic compression, confined and nonconfined compression-based reactors have been described in this review. Perfusion systems represent an attractive way of culturing constructs under dynamic conditions. Several groups showed increased matrix production using confined and unconfined systems. Development of automatic culture systems and noninvasive monitoring of matrix production will take place during the next few years in order to improve the cost affectivity of tissue-engineered products. © 2009 Springer.
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| 10. |
- Enejder, Annika, 1969, et al.
(författare)
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CARS and SHG microscopy for the characterization of bacterial cellulose
- 2009
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Ingår i: Progress in Biomedical Optics and Imaging - Proceedings of SPIE. - : SPIE. - 1605-7422. - 9780819474292 ; 7183
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- We have developed a protocol employing dual-mode non-linear microscopy for the monitoring of the biosynthesis of bacterial cellulose at a single-fiber level, with the fundamental aim to achieve a product with material properties similar to those of human blood vessels. Grown in a tubular geometry it could then be used as a natural and biocompatible source of replacement tissue in conjunction with cardiovascular surgery. The bacteria (Acetobacter xylinum) were selectively visualized based on the CH2 vibration of its organic macromolecular contents by the Coherent Anti-Stokes Raman Scattering (CARS) process and, simultaneously, the non-centrosymmetrically ordered, birefringent cellulose fibers were depicted by the Second Harmonic Generation (SHG) process. This dual-channel detection approach allows the monitoring of cellulose-fiber formation in vivo and to determine the influence of e. g. different growth conditions on fiber thickness and orientation, their assembling into higher-order structures and overall network density. The bacterial and fiber distributions were monitored in a simple microscope cultivation chamber, as well as in samples harvested during the actual fermentation process of tubular cellulose grafts. The CARS and SHG co-localization images reveal that highest bacterial population densities can be observed in the surface regions of the cellulose tissue, where the primary growth presumably takes place. The cellulose network morphology was also compared with that of human arteries and veins, from which we conclude that the cellulose matrix is comparatively homogeneous in contrast to the wavy band-like supra-formations of collagen in the native tissue. This prompts for sophisticated fermentation methods by which tunnels and pores of appropriate sizes and shapes can be introduced in the cellulose network in a controllable way. With this protocol we hope to contribute to the fundamental knowledge required for optimal production of bioengineered cellulose tissues, eventually being available for clinical use.
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