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Hypoxia increases L...
Hypoxia increases LDL oxidation and expression of 15-lipoxygenase-2 in human macrophages
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- Knutsen Rydberg, Ellen, 1969 (author)
- Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Wallenberg Laboratory,Wallenberg Lab. for Cardiovasc. Res., Sahlgrenska University Hospital, Göteborg, Sweden
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- Krettek, Alexandra, 1968 (author)
- Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Wallenberg Laboratory,Wallenberg Lab. for Cardiovasc. Res., Sahlgrenska University Hospital, Göteborg, Sweden
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- Ullström, Christina, 1950 (author)
- Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Wallenberg Laboratory,Wallenberg Lab. for Cardiovasc. Res., Sahlgrenska University Hospital, Göteborg, Sweden
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- Ekström, Karin, 1977 (author)
- Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Wallenberg Laboratory,Wallenberg Lab. for Cardiovasc. Res., Sahlgrenska University Hospital, Göteborg, Sweden
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- Svensson, Per-Arne, 1969 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för kroppssammansättning och metabolism,Institute of Internal Medicine, Dept of Body Composition and Metabolism,Res. Ctr. for Endocrinol./Metabolism, Sahlgrenska Univ. Hospital, Göteborg, Sweden
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- Carlsson, Lena M S, 1957 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för kroppssammansättning och metabolism,Institute of Internal Medicine, Dept of Body Composition and Metabolism,Res. Ctr. for Endocrinol./Metabolism, Sahlgrenska Univ. Hospital, Göteborg, Sweden
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Jönsson-Rylander, Ann-Christine (author)
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- Hansson, G.I (author)
- Bioanalytical Chemistry, AstraZeneca R and D, Mölndal, Sweden
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- McPheat, W. (author)
- Molecular Pharmacology and DMPK, AstraZeneca R and D, Mölndal, Sweden
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- Wiklund, Olov, 1943 (author)
- Gothenburg University,Göteborgs universitet,Hjärt-kärlinstitutionen,Wallenberglaboratoriet,Cardiovascular Institute,Wallenberg Laboratory,Wallenberg Lab. for Cardiovasc. Res., Sahlgrenska University Hospital, Göteborg, Sweden
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- Ohlsson, Bertil, 1954 (author)
- Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Wallenberg Laboratory,Wallenberg Lab. for Cardiovasc. Res., Sahlgrenska University Hospital, Göteborg, Sweden
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- Mattsson Hultén, Lillemor, 1951 (author)
- Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Wallenberg Laboratory,Wallenberg Lab. for Cardiovasc. Res., Sahlgrenska University Hospital, Göteborg, Sweden
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- Jönsson-Rylander, Ann-Cathrine (author)
- Molecular Pharmacology and DMPK, AstraZeneca R and D, Mölndal, Sweden
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(creator_code:org_t)
- 2004
- 2004
- English.
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In: Arterioscler Thromb Vasc Biol. ; 24:11, s. 2040-2045
- Related links:
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http://atvb.ahajourn...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Subject headings
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- OBJECTIVE: Macrophage-mediated oxidation of low-density lipoprotein (LDL) by enzymes, such as the lipoxygenases, is considered of major importance for the formation of oxidized LDL during atherogenesis. Macrophages have been identified in hypoxic areas in atherosclerotic plaques. METHODS AND RESULTS: To investigate the role of hypoxia in macrophage-mediated LDL oxidation, we incubated human monocyte-derived macrophages with LDL under normoxic (21% O2) or hypoxic (0% O2) conditions. The results showed that hypoxic macrophages oxidized LDL to a significantly higher extent than normoxic cells. Interestingly, the mRNA and protein expression of 15-lipoxygenase-2 (15-LOX-2) as well as the activity of this enzyme are elevated in macrophages incubated at hypoxia. Both the unspliced 15-LOX-2 and the spliced variant 15-LOX-2sv-a are found in macrophages. In addition, 15-LOX-2 was identified in carotid plaques in some macrophage-rich areas but was only expressed at low levels in nondiseased arteries. CONCLUSIONS: In summary, these observations show for the first time that 15-LOX-2 is expressed in hypoxic macrophages and in atherosclerotic plaques and suggest that 15-LOX-2 may be one of the factors involved in macrophage-mediated LDL oxidation at hypoxia.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Andra medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Other Basic Medicine (hsv//eng)
Keyword
- Alternative Splicing/genetics
- Anoxia/*enzymology/*pathology
- Arachidonate 15-Lipoxygenase/*biosynthesis/genetics/immunology
- Arteriosclerosis/enzymology/metabolism/pathology
- Carotid Arteries/enzymology/pathology
- Cells
- Cultured
- Enzyme Activation/genetics
- Exons/genetics
- Humans
- Immunohistochemistry/methods
- Lipoproteins
- LDL/*metabolism
- Macrophages/cytology/*enzymology
- Mammary Arteries/physiology
- Oxidation-Reduction
- Sequence Deletion/genetics
- Variation (Genetics)/genetics
- 15-lipoxygenase-2
- Medical sciences
Publication and Content Type
- ref (subject category)
- art (subject category)
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Knutsen Rydberg, ...
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Krettek, Alexand ...
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Ullström, Christ ...
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Ekström, Karin, ...
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Svensson, Per-Ar ...
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Carlsson, Lena M ...
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Jönsson-Rylander ...
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Hansson, G.I
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McPheat, W.
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Wiklund, Olov, 1 ...
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Ohlsson, Bertil, ...
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Mattsson Hultén, ...
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Jönsson-Rylander ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Other Basic Medi ...
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- Arterioscler Thr ...
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Arteriosclerosis ...
- By the university
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University of Gothenburg
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University of Skövde