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> Fager Gunnar >
Characterisation an...
Characterisation and application of antibodies specific for the long platelet-derived growth factor A and B chains.
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- Östergren Lundén, Gunnel, 1950 (author)
- Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Wallenberg Laboratory,Wallenberg Laboratory for Cardiovascular Research, The Sahlgrenska Academy at Göteborg University, Göteborg, Sweden
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- Olivas, Raquel G (author)
- Departamento de Biologı́a Celular, Universitat de Barcelona, Barcelona, Spain
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- Eftekhari, Pierre (author)
- UPR9021 C.N.R.S., Immunologie et Chimie Thérapeutiques, I.B.M.C., Strasbourg, France
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- Krettek, Alexandra, 1968 (author)
- Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Hjärt-kärlinstitutionen,Wallenberg Laboratory,Cardiovascular Institute,Wallenberg Laboratory for Cardiovascular Research, The Sahlgrenska Academy at Göteborg University, Göteborg, Sweden
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- Sanjuan, Xavier (author)
- Departamento de Biologı́a Celular, Universitat de Barcelona, Barcelona, Spain
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- Fager, Gunnar (author)
- Wallenberg Laboratory for Cardiovascular Research, The Sahlgrenska Academy at Göteborg University, Göteborg, Sweden
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- Vilaró, Senén (author)
- Departamento de Biologı́a Celular, Universitat de Barcelona, Barcelona, Spain
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- Lustig, Florentina, 1948 (author)
- Wallenberg Laboratory for Cardiovascular Research, The Sahlgrenska Academy at Göteborg University, Göteborg, Sweden
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- Hoebeke, Johan (author)
- UPR9021 C.N.R.S., Immunologie et Chimie Thérapeutiques, I.B.M.C., Strasbourg, France
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(creator_code:org_t)
- Elsevier BV, 2004
- 2004
- English.
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In: The international journal of biochemistry & cell biology. - : Elsevier BV. - 1357-2725 .- 1878-5875. ; 36:11, s. 2226-41
- Related links:
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Subject headings
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- The platelet-derived growth factor (PDGF) family comprises important mitogens for mesenchymal cells. The active dimeric form of PDGF consists of four structurally related A, B, C, and D chains. All PDGF-variants bind to PDGF-receptors. The A and B chains occur with and without basic C-terminal amino acid extensions as long (A(L) and B(L)) and short (A(S) and B(S)) isoforms. PDGF-A and -B form homo- or heterodimers. The biological relevance of short and long isoforms is unknown, although it may relate to different affinities for glycosaminoglycans of the cell glycocalix and intercellular matrix. Commercially available anti-PDGF-A and anti-PDGF-B antibodies cannot discriminate between the short and the long isoforms. Thus, to investigate the function of the long and short isoforms, we raised antibodies specific for the long A and B chain isoforms. The antibodies were affinity-purified and their properties analysed by surface plasmon resonance. Inhibition studies with different PDGF homodimers and dot-blot studies proved their high specificity for the respective isoforms. Both antibodies recognised the target PDGF homodimers complexed to the glycocalix of human arterial smooth muscle cells and human monocyte-derived macrophages. By using these specific antibodies, we were able to confirm at the protein level the synthesis of PDGF-A and -B during differentiation of human monocyte-derived macrophages and to demonstrate the presence of the PDGF-A(L) and PDGF-B(L) isoforms in human arterial tissue.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Andra medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Other Basic Medicine (hsv//eng)
Keyword
- Animals
- Antibodies
- immunology
- Carotid Arteries
- metabolism
- Cell Differentiation
- physiology
- Cells
- Cultured
- Glycocalyx
- metabolism
- Humans
- Monocytes
- cytology
- metabolism
- Myocytes
- Smooth Muscle
- cytology
- metabolism
- Platelet-Derived Growth Factor
- immunology
- metabolism
- Protein Binding
- Protein Isoforms
- immunology
- metabolism
- Rabbits
- Receptors
- Platelet-Derived Growth Factor
- metabolism
- Surface Plasmon Resonance
- GAG
- Medical sciences
Publication and Content Type
- ref (subject category)
- art (subject category)
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