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Sökning: LAR1:gu > (2010) > Tidskriftsartikel > Dohan Ehrenfest David

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1.
  • Bucci-Sabattini, Vincenzo, et al. (författare)
  • Effect of titanium implant surface nanoroughness and calcium phosphate low impregnation on bone cell activity in vitro
  • 2010
  • Ingår i: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology. - 1079-2104. ; 109:2, s. 217-224
  • Tidskriftsartikel (refereegranskat)abstract
    • Background and objective In the field of bone implant surfaces, the effects of nanoscale modifications have received significant attention. In the present study, bone cell activity on 2 implant surfaces with similar microtopography but distinct chemistry and nanotopography (sandblasted/acid-etched surface as control group, and calcium phosphate (CaP) low impregnated surface (Ossean) as test group, both from Intra-Lock, Boca Raton, FL) were evaluated.
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2.
  • Dohan Ehrenfest, David, et al. (författare)
  • Choukroun's platelet-rich fibrin (PRF) stimulates in vitro proliferation and differentiation of human oral bone mesenchymal stem cell in a dose-dependent way.
  • 2010
  • Ingår i: Archives of oral biology. - : Elsevier BV. - 1879-1506 .- 0003-9969. ; 55:3, s. 185-94
  • Tidskriftsartikel (refereegranskat)abstract
    • BACKGROUND: Choukroun's platelet-rich fibrin (PRF) is an autologous leukocyte- and platelet-rich fibrin biomaterial. The purpose of this study was to analyse the in vitro effects of PRF on human bone mesenchymal stem cells (BMSC), harvested in the oral cavity after preimplant endosteal stimulation. MATERIALS AND METHODS: BMSCs from primary cultures were cultivated with or without a PRF membrane originating from the same donor as for the cells, in proliferation or osteoblastic differentiation conditions. After 7 days, the PRF membranes were removed. A series of cultures were performed using 2 PRF membranes, in order to measure the dose-dependent effect. Cell counts, cytotoxicity tests, alkaline phosphatase (ALP) activity quantification, Von Kossa staining and mineralisation nodules counts were performed at 3, 7, 14, 21 and 28 days. A last independent series was carried on up to 14 days, for a morphological scanning electron microscope (SEM) observation. RESULTS: PRF generated a significant stimulation of the BMSC proliferation and differentiation throughout the experimental period. This effect was dose-dependent during the first weeks in normal conditions, and during the whole experimentation in differentiation conditions. The cultures without PRF in differentiation conditions did not rise above the degree of differentiation of the cultures in normal conditions with 1 or 2 PRF up to the 14th and 28th day, respectively. The SEM culture analysis at day 14 allowed to show the mineralisation nodules which were more numerous and more structured in the groups with PRF compared to the control groups. DISCUSSION AND CONCLUSIONS: This double contradictory proliferation/differentiation result may be due to the numerous components of PRF, particularly the presence of leukocytes: any culture with PRF is in fact a coculture with leukocytes. It could be the source of differential geographic regulation processes within the culture. The combination of oral BMSC and PRF might offer many potential clinical and biotechnological applications, and deserves new studies.
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  • Lemo, Niksa, et al. (författare)
  • Cutaneous reepithelialization and wound contraction after skin biopsies in rabbits: a mathematical model for healing and remodelling index
  • 2010
  • Ingår i: VETERINARSKI ARHIV. - 0372-5480. ; 80:5, s. 637-652
  • Tidskriftsartikel (refereegranskat)abstract
    • The objective of this study was to develop a standard operating procedure for the analysis of skin wound healing using histomorphometrical measurements and mathematical data analyses. The mathematical model is derived from observations of normal cutaneous healing in the rabbit. It is designed to allow a simple scoring of the major steps of healing and remodelling. Full-thickness punch biopsies were performed on the skin of the back of New Zealand-white rabbits and healing was analyzed by histopathological examination after 2, 5, 9 and 14 days, using different staining techniques. Histomorphological measurements were also made. The thickness of the epidermis and neo-epidermis were compared. Several indices relative to wound severity and contraction were computed in an attempt to define a global healing index. A remodelling index was calculated based on a colorimetric analysis with Mallory Trichrome staining and hair migration. The changes in index values seemed to correlate with the histopathological analysis. No material flaws appeared when this model was applied to the natural healing process. This model was developed for scoring and accurate comparative evaluation of the effects of various treatments, biomaterials or pharmacological preparations on soft tissue healing and remodelling in rabbits. Although the healing of cutaneous wounds in rabbits differs from that in humans, this model may still be relevant for screening new wound healing preparations.
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9.
  • Mouhyi, Jaafar, et al. (författare)
  • Mouthwash solutions containing microencapsulated natural extracts: Clinical results on dental plaque and gingivitis
  • 2010
  • Ingår i: Revue de Stomatologie et de Chirurgie Maxillo-faciale. - 0035-1768. ; 111:3, s. 144-147
  • Tidskriftsartikel (refereegranskat)abstract
    • ntroduction New mouthwash solutions containing microencapsulated natural extracts have been developed. Besides antiseptic activity, these solutions have antioxidant and immunoregulatory properties on oral tissues. The objective of this preliminary clinical study was to assess the efficiency of a mouthwash containing microencapsulated natural extracts (GingiNat, LoB5 Foundation, Paris, France) on gingivitis. Patients and methods Twenty volunteers (37 ± 2 years) with significant gingivitis (bleeding when tooth-brushing) used a 6% GingiNat® mouthwash solution, two to three times per day (according to oral hygiene habits) for 21 days in addition to their usual oral care. Each volunteer was examined at day 0, 4, 7 and 21. The Löe and Silness Plaque Index, the Russel Periodontal Index, a breath index (halitosis), and oral tissue tolerance were assessed. Finally, each volunteer filled in a daily follow-up form and answered a questionnaire on tolerance and acceptability. Results A significant decrease of the dental plaque index was observed on day 4, 7, and 21, in respectively 29, 48 and 71% of volunteers. This decrease reached 18% on day 4 (p = 0.014), 32% on day 7 (p = 0.002), and 47% on day 21 (p < 0.001). A significant decrease of the periodental index was observed on day 4, 7 and 21, in respectively 52, 81 and 95% of volunteers. This decrease reached 30% (p = 0.001) on day 4, 49% (p < 0.001) on day 7, and 78% (p < 0.001) on day 21. A significant improvement of the breath index was noted on day 4, 7, and 21, in respectively 43, 52 and 48% of volunteers. This improvement reached 29% (p = 0.004) on day 4, 35% (p = 0.001) on day 7, and 32% (p = 0.002) on day 21. The mouthwash was well tolerated. The patients liked its effectiveness and organoleptic properties. All patients expressed the wish to continue using this solution. Discussion The GingiNat® mouthwash solution at 6% had a significant efficiency on plaque, gingivitis, and halitosis after 21 days of use. This makes it a good complementary treatment for gingivitis. Tolerance and acceptability were good despite the long and repeated use. Further studies are needed to have a detailed analysis of its efficiency in the long run and on patients presenting with various forms of periodontitis.
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10.
  • Paranque, Armand-Régis, et al. (författare)
  • Esthetic and functional reconstruction of the post-tumoral interrupted mandible with double-barrel fibular free flap graft: rationale for a prosthodontic approach.
  • 2010
  • Ingår i: The Journal of oral implantology. - 0160-6972.
  • Tidskriftsartikel (refereegranskat)abstract
    • Abstract Background. Reconstruction after substantial osseous, cutaneous and muscular tissue loss following a mandibular resection is a challenge. The use of a fibular free flap is an outstanding - but delicate - treatment option. These grafts, using the double-barrel technique, can achieve an almost complete reconstruction of the mandibular defect. The challenge posed by these treatments is to achieve an end result that is both functional and esthetically pleasing; an endeavour that requires a defined prosthetic plan prior to complete microsurgical reconstruction.Materials and Methods. Using a detailed clinical case, this article discusses the importance of planning the mandible reconstruction with double-barrel fibular graft in view of an implant-supported fixed partial denture. Immediate implant loading was even possible in this case.Results. This approach allows to improve the final esthetic and functional result of such a complex rehabilitation.Conclusions. Maxillofacial reconstructive surgery should seek to establish a near-as-normal anatomical situation that will allow a permanent implant rehabilitation that is both esthetic and durable.
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