| 1. |
- Hagström, Åke, et al.
(författare)
-
Composition and Seasonality of Membrane Transporters in Marine Picoplankton
- 2021
-
Ingår i: Frontiers in Microbiology. - : Frontiers Media SA. - 1664-302X. ; 12
-
Tidskriftsartikel (refereegranskat)abstract
- In this study, we examined transporter genes in metagenomic and metatranscriptomic data from a time-series survey in the temperate marine environment of the Baltic Sea. We analyzed the abundance and taxonomic distribution of transporters in the 3 mu m-0.2 mu m size fraction comprising prokaryotes and some picoeukaryotes. The presence of specific transporter traits was shown to be guiding the succession of these microorganisms. A limited number of taxa were associated with the dominant transporter proteins that were identified for the nine key substrate categories for microbial growth. Throughout the year, the microbial taxa at the level of order showed highly similar patterns in terms of transporter traits. The distribution of transporters stayed the same, irrespective of the abundance of each taxon. This would suggest that the distribution pattern of transporters depends on the bacterial groups being dominant at a given time of the year. Also, we find notable numbers of secretion proteins that may allow marine bacteria to infect and kill prey organisms thus releasing nutrients. Finally, we demonstrate that transporter proteins may provide clues to the relative importance of biogeochemical processes, and we suggest that virtual transporter functionalities may become important components in future population dynamics models.
|
|
| 2. |
- Fridolfsson, Emil, et al.
(författare)
-
Multiyear analysis uncovers coordinated seasonality in stocks and composition of the planktonic food web in the Baltic Sea proper
- 2023
-
Ingår i: Scientific Reports. - London : Springer Nature. - 2045-2322. ; 13:1
-
Tidskriftsartikel (refereegranskat)abstract
- The planktonic realm from bacteria to zooplankton provides the baseline for pelagic aquatic food webs. However, multiple trophic levels are seldomly included in time series studies, hampering a holistic understanding of the influence of seasonal dynamics and species interactions on food web structure and biogeochemical cycles. Here, we investigated plankton community composition, focusing on bacterio-, phyto- and large mesozooplankton, and how biotic and abiotic factors correlate at the Linnaeus Microbial Observatory (LMO) station in the Baltic Sea from 2011 to 2018. Plankton communities structures showed pronounced dynamic shifts with recurring patterns. Summarizing the parts of the planktonic microbial food web studied here to total carbon, a picture emerges with phytoplankton consistently contributing > 39% while bacterio- and large mesozooplankton contributed ~ 30% and ~ 7%, respectively, during summer. Cyanophyceae, Actinobacteria, Bacteroidetes, and Proteobacteria were important groups among the prokaryotes. Importantly, Dinophyceae, and not Bacillariophyceae, dominated the autotrophic spring bloom whereas Litostomatea (ciliates) and Appendicularia contributed significantly to the consumer entities together with the more traditionally observed mesozooplankton, Copepoda and Cladocera. Our findings of seasonality in both plankton composition and carbon stocks emphasize the importance of time series analyses of food web structure for characterizing the regulation of biogeochemical cycles and appropriately constraining ecosystem models.
|
|
| 3. |
- Aguilera, Anabella, et al.
(författare)
-
Ecophysiological analysis reveals distinct environmental preferences in closely related Baltic Sea picocyanobacteria
- 2023
-
Ingår i: Environmental Microbiology. - Chichester : John Wiley & Sons. - 1462-2912 .- 1462-2920. ; 25:9, s. 1674-1695
-
Tidskriftsartikel (refereegranskat)abstract
- Cluster 5 picocyanobacteria significantly contribute to primary productivity in aquatic ecosystems. Estuarine populations are highly diverse and consist of many co-occurring strains, but their physiology remains largely understudied. In this study, we characterized 17 novel estuarine picocyanobacterial strains. Phylogenetic analysis of the 16S rRNA and pigment genes (cpcBandcpeBA) uncovered multiple estuarine and freshwater-related clusters and pigment types. Assays with five representative strains (three phycocyanin rich and two phycoerythrin rich) under temperature (10–30°C), light(10–190 μmol photons m-2s-1), and salinity (2–14 PSU) gradients revealed distinct growth optima and tolerance, indicating that genetic variability was accompanied by physiological diversity. Adaptability to environmental conditions was associated with differential pigment content and photosynthetic performance. Amplicon sequence variants at a coastal and an offshore station linked population dynamics with phylogenetic clusters, supporting that strains isolated in this study represent key ecotypes within the Baltic Sea picocyanobacterial community. The functional diversity found within strains with the same pigment type suggests that understanding estuarine picocyanobacterial ecology requires analysis beyond the phycocyanin and phycoerythrin divide. This new knowledge of the environmental preferences in estuarine picocyanobacteria is important for understanding and evaluating productivity in current and future ecosystems.
|
|
| 4. |
- Laber, Christien P., et al.
(författare)
-
Seasonal and Spatial Variations in Synechococcus Abundance and Diversity Throughout the Gullmar Fjord, Swedish Skagerrak
- 2022
-
Ingår i: Frontiers in Microbiology. - Lausanne : Frontiers Media S.A.. - 1664-302X. ; 13
-
Tidskriftsartikel (refereegranskat)abstract
- The picophytoplankton Synechococcus is a globally abundant autotroph that contributes significantly to primary production in the oceans and coastal areas. These cyanobacteria constitute a diverse genus of organisms that have developed independent niche spaces throughout aquatic environments. Here, we use the 16S V3-V4 rRNA gene region and flow cytometry to explore the diversity of Synechococcus within the picophytoplankton community in the Gullmar Fjord, on the west coast of Sweden. We conducted a station-based 1-year time series and two transect studies of the fjord. Our analysis revealed that within the large number of Synechococcus amplicon sequence variants (ASVs; 239 in total), prevalent ASVs phylogenetically clustered with clade representatives in both marine subcluster 5.1 and 5.2. The near-surface composition of ASVs shifted from spring to summer, when a 5.1 subcluster dominated community developed along with elevated Synechococcus abundances up to 9.3 x 10(4) cells ml(-1). This seasonal dominance by subcluster 5.1 was observed over the length of the fjord (25 km), where shifts in community composition were associated with increasing depth. Unexpectedly, the community shift was not associated with changes in salinity. Synechococcus abundance dynamics also differed from that of the photosynthetic picoeukaryote community. These results highlight how seasonal variations in environmental conditions influence the dynamics of Synechococcus clades in a high latitude threshold fjord.
|
|
| 5. |
- Lindh, Markus V., et al.
(författare)
-
Metapopulation theory identifies biogeographical patterns among core and satellite marine bacteria scaling from tens to thousands of kilometers
- 2017
-
Ingår i: Environmental Microbiology. - : Wiley-Blackwell. - 1462-2912 .- 1462-2920. ; 19:3, s. 1222-1236
-
Tidskriftsartikel (refereegranskat)abstract
- Metapopulation theory developed in terrestrial ecology provides applicable frameworks for interpreting the role of local and regional processes in shaping species distribution patterns. Yet, empirical testing of metapopulation models on microbial communities is essentially lacking. We determined regional bacterioplankton dynamics from monthly transect sampling in the Baltic Sea Proper using 16S rRNA gene sequencing. A strong positive trend was found between local relative abundance and occupancy of populations. Notably, the occupancy-frequency distributions were significantly bimodal with a satellite mode of rare endemic populations and a core mode of abundant cosmopolitan populations (e.g. Synechococcus, SAR11 and SAR86 clade members). Temporal changes in population distributions supported several theoretical frameworks. Still, bimodality was found among bacterioplankton communities across the entire Baltic Sea, and was also frequent in globally distributed datasets. Datasets spanning waters with widely different physicochemical characteristics or environmental gradients typically lacked significant bimodal patterns. When such datasets were divided into subsets with coherent environmental conditions, bimodal patterns emerged, highlighting the importance of positive feedbacks between local abundance and occupancy within specific biomes. Thus, metapopulation theory applied to microbial biogeography can provide novel insights into the mechanisms governing shifts in biodiversity resulting from natural or anthropogenically induced changes in the environment.
|
|
| 6. |
- Alegria Zufia, Javier, Ph.D. 1992-, et al.
(författare)
-
Growth and mortality rates of picophytoplankton in the Baltic Sea Proper
- 2024
-
Ingår i: Marine Ecology Progress Series. - Oldendorf : Inter-Research. - 0171-8630 .- 1616-1599. ; 735, s. 63-76
-
Tidskriftsartikel (refereegranskat)abstract
- Picophytoplankton (<2 µm diameter), a diverse group of picocyanobacteria and photosynthetic picoeukaryotes, are significant contributors to primary production. Predatory mortality controls picophytoplankton biomass and thereby energy transfer in the marine food web. The 2 major pathways of picophytoplankton mortality are grazing and viral lysis. Grazing passes carbon directly to higher trophic levels, while lysis products are passed into the viral loop. Picophytoplankton are abundant in the Baltic Sea but little is known about their predatory mortality. Using a modification of the dilution approach, we calculated growth and mortality rates of picophytoplankton and studied the effect of predation on community structure during late August and September. The experiments were conducted coinciding with the peak in picophytoplankton abundance (similar to 10(5) cells ml(-1)) at the Linnaeus Microbial Observatory in the Baltic Sea Proper. The results showed that grazing is an important controller of picocyanobacteria and photosynthetic picoeukaryote populations, while no significant viral lysis effect was detected. Grazing on picocyanobacteria was proportional to growth rates, while grazing on photosynthetic picoeukaryotes exceeded growth. Selective grazing of phylogenetically distinct picocyanobacterial clades had a significant effect on community structure, suggesting that grazing has an impact on the seasonal dynamics of co-occurring clades. Picocyanobacteria had a higher carbon transfer contribution to higher trophic levels than photosynthetic picoeukaryotes at the time of the experiments. The study shows that picophytoplankton are important contributors to carbon cycling in the Baltic Sea microbial food web and should be considered for future ecological models.
|
|
| 7. |
- Borschel, Christian, et al.
(författare)
-
A New Route toward Semiconductor Nanospintronics : Highly Mn-Doped GaAs Nanowires Realized by Ion-Implantation under Dynamic Annealing Conditions
- 2011
-
Ingår i: Nano letters (Print). - Washington : American Chemical Society (ACS). - 1530-6984 .- 1530-6992. ; 11:9, s. 3935-3940
-
Tidskriftsartikel (refereegranskat)abstract
- We report on highly Mn-doped GaAs nanowires (NWs) of high crystalline quality fabricated by ion beam implantation, a technique that allows doping concentrations beyond the equilibrium solubility limit. We studied two approaches for the preparation of Mn-doped GaAs NWs: First, ion implantation at room temperature with subsequent annealing resulted in polycrystalline NWs and phase segregation of MnAs and GaAs. The second approach was ion implantation at elevated temperatures. In this case, the single-crystallinity of the GaAs NWs was maintained, and crystalline, highly Mn-doped GaAs NWs were obtained. The electrical resistance of such NWs dropped with increasing temperature (activation energy about 70 meV). Corresponding magnetoresistance measurements showed a decrease at low temperatures, indicating paramagnetism. Our findings suggest possibilities for future applications where dense arrays of GaMnAs nanowires may be used as a new kind of magnetic material system.
|
|
| 8. |
- Khakpour, Narges, et al.
(författare)
-
Formal modeling of evolving self-adaptive systems
- 2012
-
Ingår i: Science of Computer Programming. - Amsterdam : Elsevier. - 0167-6423 .- 1872-7964. ; 78:1, s. 3-26
-
Tidskriftsartikel (refereegranskat)abstract
- In this paper, we present a formal model, named PobSAM (Policy-based Self-Adaptive Model), for developing and modeling self-adaptive evolving systems. In this model, policies are used as a mechanism to direct and adapt the behavior of self-adaptive systems. A PobSAM model is a collection of autonomous managers and managed actors. The managed actors are dedicated to the functional behavior while the autonomous managers govern the behavior of managed actors by enforcing suitable policies. A manager has a set of configurations including two types of policies: governing policies and adaptation policies. To adapt the system behavior in response to the changes, the managers switch among different configurations. We employ the combination of an algebraic formalism and an actor-based model to specify this model formally. Managed actors are expressed by an actor model. Managers are modeled as meta-actors whose configurations are described using a multi-sorted algebra called CA. We provide an operational semantics for PobSAM using labeled transition systems. Furthermore, we provide behavioral equivalence of different sorts of CA in terms of splitting bisimulation and prioritized splitting bisimulation. Equivalent managers send the same set of messages to the actors. Using our behavioral equivalence theory, we can prove that the overall behavior of the system is preserved by substituting a manager by an equivalent one.
|
|
| 9. |
- Khakpour, Narges, et al.
(författare)
-
PobSAM : policy-based managing of actors in self-adaptive systems
- 2010
-
Ingår i: Proceedings of the 6th International Workshop on Formal Aspects of Component Software (FACS’09, Eindhoven, The Netherlands, November 2-3, 2009). - Amsterdam : Elsevier. ; 263, s. 129-143
-
Konferensbidrag (refereegranskat)
|
|
| 10. |
- Liu, Ruisheng S., et al.
(författare)
-
Magnetoresistance studies on CoAl OX Au and CoAl OX NiAu tunnel structures
- 2008
-
Ingår i: Applied Physics Letters. - New York : American Institute of Physics (AIP). - 0003-6951 .- 1077-3118. ; 93:20, s. 203107-203107-3
-
Tidskriftsartikel (refereegranskat)abstract
- We report on magnetoresistance (MR) studies on CoAl OX Au and CoAl OX NiAu magnetic tunnel junctions. In spite of the fact that the difference between the two samples is merely a 3 nm thick Ni layer, there is a sharp contrast in MR behavior indicating that the electronic structure at the interface between the ferromagnetic electrodes and the insulating barrier dominates the MR signal. The former sample exhibits a clear tunneling anisotropic MR (TAMR), with the characteristic correlation between resistance and current direction, in contrast to the latter sample which displays a conventional tunneling MR (TMR) dominated by the relative orientation between the magnetization directions of the two electrodes. In addition, the TAMR has a much stronger temperature dependence than the TMR, indicating a much faster drop-off of the tunneling density of states anisotropy than the tunneling electron spin polarization with increasing temperature. Finally, we propose a possible simple way to distinguish TAMR from normal TMR by measuring the resistance of the device at different angles of the external magnetic field. 2008 American Institute of Physics.
|
|
