↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Tyck till om SwePub Sök här!

Träfflista för sökning "LAR1:lnu ;pers:(Ohlson Sten)"

Sökning: LAR1:lnu > Ohlson Sten

Resultat 1-10 av 103

Sortera/gruppera träfflistan

Sortering: Träffar per sida:

Numrering	Referens	Omslagsbild	Hitta
1.	Aldén, Anna, et al. (författare) HPLC analysis of carbohydrate deficient transferrin isoforms isolated by the Axis-Shield %CDT method 2005 Ingår i: Clinica chimica acta. ; 356 (1-2), s. 143-146 Tidskriftsartikel (refereegranskat)
2.	Aldén, Anna, et al. (författare) Porcine platelet lysate as a supplement for animal cell culture 2007 Ingår i: Cytotechnology (Dordrecht). - : Springer Science and Business Media LLC. - 0920-9069 .- 1573-0778. ; 55, s. 3-8 Tidskriftsartikel (refereegranskat)
3.	Andersson, Håkan S., et al. (författare) Study of the Nature of Recognition in Molecularly Imprinted Polymers 1996 Ingår i: J. Mol. Recogn.. ; 9, p 675-682 Tidskriftsartikel (refereegranskat)
4.	Andersson, Håkan S., 1967-, et al. (författare) Study of Weak Affinity Interactions in Molecularly Imprinted Polymers 1995 Ingår i: Journal of Molecular Recognition. - : Wiley. - 0952-3499 .- 1099-1352. ; 8:3, s. 231- Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
5.	Bergström, Maria, et al. (författare) Cholera toxin inhibitors studied with High-performance liquid affinity chromatography: arobust method to evaluate receptor-ligand interactions 2009 Ingår i: Chemical Biology and Drug Design. - : Wiley. - 1747-0277 .- 1747-0285. ; 73:1, s. 132-141 Tidskriftsartikel (refereegranskat)abstract Anti-adhesion drugs may be an alternative to antibiotics to control infection of micro-organisms. The well-characterized interaction between cholera toxin and the cellular glycolipid GM1 makes it an attractive model for inhibition studies in general. In this report, we demonstrate a high-performance liquid affinity chromatography approach called weak affinity chromatography to evaluate cholera toxin inhibitors. The cholera toxin B-subunit was covalently coupled to porous silica and a (weak) affinity column was produced. The K(D) values of galactose and meta-nitrophenyl alpha-d-galactoside were determined with weak affinity chromatography to be 52 and 1 mm, respectively, which agree well with IC(50) values previously reported. To increase inhibition potency multivalent inhibitors have been developed and the interaction with multivalent glycopolypeptides was also evaluated. The affinity of these compounds was found to correlate with the galactoside content but K(D) values were not obtained because of the inhomogeneous response and slow off-rate from multivalent interactions. Despite the limitations in obtaining direct K(D) values of the multivalent galactopolypeptides, weak affinity chromatography represents an additional and valuable tool in the evaluation of monovalent as well as multivalent cholera toxin inhibitors. It offers multiple advantages, such as a low sample consumption, high reproducibility and short analysis time, which are often not observed in other methods of analysis.
6.	Bergström, Maria, et al. (författare) Elucidating the selectivity of recombinant forms of Aleuria aurantia lectin using weak affinity chromatography 2012 Ingår i: Journal of chromatography. B. - : Elsevier. - 1570-0232 .- 1873-376X. ; 885, s. 66-72 Tidskriftsartikel (refereegranskat)abstract Aberrant glycosylation is connected to several pathological conditions and lectins are useful tools to characterize glycosylated biomarkers. The Aleuria aurantia lectin (AAL) is of special interest since it interacts with all types of fucosylated saccharides. AAL has been expressed in Escherichia coil as a fully functional recombinant protein. Engineered variants of AAL have been developed with the aim of creating monovalent lectins with more homogenous binding characteristics. Four different forms of AAL were studied in the present work: native AAL purified from A. aurantia mushrooms, recombinant AAL dimer, recombinant AAL monomer and recombinant AAL site 2 (S2-AAL). The affinities of these AAL forms toward a number of saccharides were determined with weak affinity chromatography (WAC). Disaccharides with fucose linked alpha 1-3 to GIcNAc interacted with higher affinity compared to fucose linked alpha 1-6 or alpha 1-4 and the obtained dissociation constants (K-d) were in the range of 10 mu M for all AAL forms. Tetra- and pentasaccharides with fucose in alpha 1-2, alpha 1-3 or alpha 1-4 had K-d values ranging from 0.1 to 7 mM while a large alpha 1-6 fucosylated oligosaccharide had a K-d of about 20 mu M. The recombinant multivalent AAL forms and native AAL exhibited similar affinities toward all saccharides, but S2-AAL had a lower affinity especially regarding a sialic acid containing fucosylated saccharide. It was demonstrated that WAC is a valuable technique in determining the detailed binding profile of the lectins. Specific advantages with WAC include a low consumption of non-labeled saccharides, possibility to analyze mixtures and a simple procedure using standard HPLC equipment.
7.	Bergström, Maria, et al. (författare) Lectin Affinity Capillary Electrophoresis in Glycoform Analysis Applying the Partial Filling Technique 2004 Ingår i: Journal of Chromatography B. ; 809, s. 323-329 Tidskriftsartikel (refereegranskat)
8.	Bergström, Maria, et al. (författare) Use of perfusive supports in weak affinity chromatography (WAC) 2001 Ingår i: Bio-chromatography. ; 6, s. 163-172 Tidskriftsartikel (refereegranskat)
9.	Bergström, Maria, et al. (författare) Use of weak monoclonal antibodies for affinity chromatography 1998 Ingår i: Journal of Molecular Recognition. ; 11, s. 110-113 Tidskriftsartikel (refereegranskat)
10.	Bratt, T, et al. (författare) Interactions between neutrophil gelatinase-associated lipocalin and natural hydrophobic ligands 1999 Ingår i: Biochimica et biophysica acta. ; 1472, s. 262-266 Tidskriftsartikel (refereegranskat)

Skapa referenser, mejla, bekava och länka

Länka till träfflistan

Resultat 1-10 av 103

Avgränsa träffmängd

Typ av publikation: tidskriftsartikel (79); patent (11); bokkapitel (5); doktorsavhandling (4); bok (2); konferensbidrag (2); visa fler...; visa färre...

Typ av innehåll: refereegranskat (80); övrigt vetenskapligt/konstnärligt (12); populärvet., debatt m.m. (11)

Författare/redaktör: Ohlson, Sten (101)Ta bort avgränsningen; Bergström, Maria (17); Mosbach, K (15); Larsson, P. O. (15); Isaksson, Roland (10); Duong-Thi, Minh-Dao (8); visa fler...; Påhlsson, Peter (7); Lundblad, Arne (7); Fex, Tomas (7); Zopf, David (7); Glad, M (6); Strandh, Magnus (6); Leickt, Lisa (6); Hansson, L (5); Engström, Henrik (5); Meiby, Elinor (5); Andersson, Per Ola (4); Aldén, Anna (3); Rydén, I (3); Grubb, A (3); Johansson, Reine (3); Ohlin, M. (3); Holmqvist, O. (3); Månsson, M O (3); Shoravi, Siamak (3); Niss, U (3); Bergström, Gunnar (2); Edwards, Katarina (2); Nilsson, I. (2); Andersson, Håkan S. (2); Agmo Hernández, Víct ... (2); Nilsson, Mikael (2); Persson, A. (2); Lindberg, A Michael (2); Mandenius, Carl-Fred ... (2); Koch-Schmidt, Ann-Ch ... (2); Christensson, K (2); Åström, Eva (2); Freiburghaus, C. (2); Holmberg, A (2); Bratt, T (2); Ohlson, Sten, Profes ... (2); Gregorius, Klaus (2); Liljeblad, M. (2); Jungar, C (2); Mandenius, C-F (2); Niss, Ulf (2); Wang, W. T. (2); Fex, T (2); Alfredsson, N (2); visa färre...

Lärosäte: Linnéuniversitetet (103); Linköpings universitet (2); Göteborgs universitet (1); Uppsala universitet (1); Stockholms universitet (1); Mälardalens universitet (1); visa fler...; Chalmers tekniska högskola (1); Karolinska Institutet (1); visa färre...

Språk: Engelska (102); Svenska (1)

Forskningsämne (UKÄ/SCB): Naturvetenskap (22); Teknik (8); Medicin och hälsovetenskap (6)

År

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

Copyright © LIBRIS - Nationella bibliotekssystem
LIBRIS.kb.se

pil uppåt

Stäng

Kopiera och spara länken för att återkomma till aktuell vy