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- Axelsson Olsson, Diana, et al.
(författare)
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Acanthamoeba-Campylobacter coculture as a novel method for enrichment of Campylobacter species
- 2007
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Ingår i: Applied and Environmental Microbiology. - 0099-2240 .- 1098-5336. ; 73:21, s. 6864-6869
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Tidskriftsartikel (refereegranskat)abstract
- In this study, we present a novel method to isolate and enrich low concentrations of Campylobacter pathogens. This method, Acanthamoeba-Campylobacter coculture (ACC), is based on the intracellular survival and multiplication of Campylobacter species in the free-living protozoan Acanthamoeba polyphaga. Four of the Campylobacter species relevant to humans and livestock, Campylobacter jejuni, C. coli, C. lari, and C. hyointestinalis, were effectively enriched by the coculture method, with growth rates comparable to those observed in other Campylobacter enrichment media. Studying six strains of C. jejuni isolated from different sources, we found that all of the strains could be enriched from an inoculum of fewer than 10 bacteria. The sensitivity of the ACC method was not negatively affected by the use of Campylobacter-selective antibiotics in the culture medium, but these were effective in suppressing the growth of seven different bacterial species added at a concentration of 10(4) CFU/ml of each species as deliberate contamination. The ACC method has advantages over other enrichment methods as it is not dependent on a microaerobic milieu and does not require the use of blood or other oxygen-quenching agents. Our study found the ACC method to be a promising tool for the enrichment of Campylobacter species, particularly from water samples with low bacterial concentrations.
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| 2. |
- Lutic, D, et al.
(författare)
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Catalytic properties of oxide nanoparticles applied in gas sensors
- 2007
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Ingår i: Topics in Catalysis. - : Springer Science and Business Media LLC. - 1572-9028 .- 1022-5528. ; 45:1-4, s. 105-109
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Tidskriftsartikel (refereegranskat)abstract
- A series of gas sensing layers based on indium oxide doped with gold were prepared by using the aerosol technology for deposition as the active contact layer in a metal oxide semiconductor capacitive device. The interaction between the measured species and the insulator surface was quantified as the voltage changes at a constant capacitance of the device. The sensor properties were investigated in the presence of H2, CO, NH3, NO, NO2 and C3H6 at temperatures between 100–400 °C. Significant differences in the morphology of the layer and its sensitivity were noted for different preparation methods and different gas environments.
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| 3. |
- Svedin, Carl Göran, 1947-, et al.
(författare)
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Selling sex in a population-based study of high school seniors in Sweden : Demographic and psychosocial correlates
- 2007
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Ingår i: Archives of Sexual Behavior. - : Springer Science and Business Media LLC. - 0004-0002 .- 1573-2800. ; 36:1, s. 21-32
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Tidskriftsartikel (refereegranskat)abstract
- Adolescents reporting selling sex for payment were studied with respect to socioeconomic background, perceived mental health and health behavior, antisocial behavior, sexual experiences, including sexual abuse and abusive behaviors, and the use of pornography. The sample consisted of a representative sample of 4,339 students (response rate, 77.2%) from the third year of upper secondary schools in five Swedish cities. Of the participating adolescents, 1.8% of the boys and 1.0% of the girls indicated that they had sold sex for money or other reimbursements. Selling sex was associated with having an immigrant background, higher level of unemployment in the family, and studying a practical/vocational program. Adolescents with the experience of selling sex had an increased risk for different psychosocial problems, such as poorer mental health, weekly use of alcohol, and antisocial behaviors. The adolescents selling sex were also characterized by having had a greater number of sexual experiences, a greater preoccupation with sex, relatively early sexual debut, and experience with sexual abuse as victim and/or perpetrator. The index group students watched pornography more frequently than the majority and they also watched more deviant forms of pornography. The significance of the higher frequency among boys and the gray zone between normal sexual exploration and prostitution are discussed.
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