| 1. |
- Bucardo, Filemon, et al.
(författare)
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Genetic susceptibility to symptomatic norovirus infection in Nicaragua. : norovirus susceptibility in Nicaragua
- 2009
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Ingår i: Journal of medical virology. - : Wiley. - 1096-9071 .- 0146-6615. ; 81:4, s. 728-35
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Tidskriftsartikel (refereegranskat)abstract
- Host genetic resistance to Norovirus (NoV) has been observed in challenge and outbreak studies in populations from Europe, Asia, and USA. In this study, we have investigated if histo-blood group antigens can predict susceptibility to diarrhea caused by NoV in Nicaragua, Central America, and if this can be reflected in antibody-prevalence and titer to NoV among individuals with different histo-blood group antigen phenotypes. Investigation of 28 individuals infected with NoV and 131 population controls revealed 6% of non-secretors in the population and nil non-secretors among patients infected with NoV, suggesting that non-secretors may be protected against NoV disease in Nicaragua. Surprisingly, 25% of the population was Lewis negative (Le(a-b-)). NoV infections with genogroup I (GI) and GII occurred irrespective of Lewis genotype, but none of the Lewis a positive (Le(a + b-)) were infected. The globally dominating GII.4 virus infected individuals of all blood groups except AB (n = 5), while the GI viruses (n = 4) infected only blood type O individuals. Furthermore, O blood types were susceptible to infections with GI.4, GII.4, GII.7, GII.17, and GII.18-Nica viruses, suggesting that secretors with blood type O are susceptible (OR = 1.52) and non-secretors resistant. The overall antibody-prevalence to NoV GII.3 VLP was 62% with the highest prevalence among blood type B carriers (70%) followed by A (68%) and O (62%). All four investigated individuals carrying blood type AB were antibody-negative. Among secretors, 63% were antibody-positive compared to 33% among non-secretors (P = 0.151). This study extends previous knowledge about the histo-blood group antigens role in NoV disease in a population with different genetic background than North American and European.
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| 2. |
- Mendoza, J. A., et al.
(författare)
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Assessment of groundwater vulnerability in the Rio Artiguas basin, Nicaragua
- 2006
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Ingår i: Environmental Geology. - : Springer Science and Business Media LLC. - 0943-0105 .- 1432-0495. ; 50:4, s. 569-580
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Tidskriftsartikel (refereegranskat)abstract
- The Rio Artiguas basin in central Nicaragua shows a distinctive case of environmental deterioration due to anthropogenic activities. Heavy metals used in gold mining and other wastes are continuously released into the rivers, representing a threat to the water quality. This article aims to evaluate the groundwater intrinsic vulnerability in the Rio Artiguas basin and to provide information for sustainable use of water resources. The DRASTIC and GOD methods were used to analyse the relative pollution potential within the basin. DRASTIC was modified to include the degree of influence that geological structures have on the vulnerability. Moderate vulnerability areas cover most of the basin along stream valleys and lowlands, increasing downstream in the basin. The resulting vulnerability maps show that the limited groundwater resources are susceptible to surface water pollution as high vulnerability areas converge along the river valleys.
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| 3. |
- Espinoza, F., et al.
(författare)
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Shifts of rotavirus G and P types in Nicaragua - 2001-2003
- 2006
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Ingår i: The Pediatric Infectious Disease Journal. - : Ovid Technologies (Wolters Kluwer Health). - 0891-3668 .- 1532-0987. ; 25:11, s. 1078-1080
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Tidskriftsartikel (refereegranskat)abstract
- The present study reports the diversity of rotavirus strains circulating in León, Nicaragua during three years. There was a shift of G and P genotypes with increment of one specific genotype during the second most important peak of diarrhea occurring in the beginning of every year. © 2006 Lippincott Williams & Wilkins, Inc.
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| 4. |
- Lebbad, Marianne, et al.
(författare)
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Dominance of Giardia assemblage B in León, Nicaragua.
- 2008
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Ingår i: Acta Tropica. - : Elsevier BV. - 0001-706X .- 1873-6254. ; 106:1, s. 44-53
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Tidskriftsartikel (refereegranskat)abstract
- Giardiasis is a major problem in León, Nicaragua, yet despite this no data are available regarding the prevalence of different Giardia genotypes in this area. To address this question, a molecular analysis of Giardia isolates from humans and dogs living in the same area in León, Nicaragua was performed. Giardia isolates from 119 Nicaraguan patients and 8 dogs were successfully genotyped using single and/or nested beta-giardin PCR with subsequent restriction length fragment polymorphism (RFLP) analysis. The analyses of human samples yielded 94 (79%) assemblage B isolates and 25 (21%) assemblage A isolates. Only the non-human-associated assemblages C and D were found in the dog samples. Sixteen isolates with assemblage A pattern, 26 isolates with assemblage B pattern and all dog isolates were further characterized by sequencing the nested beta-giardin PCR product and by molecular analyses of the glutamate dehydrogenase (gdh) gene. Within the study area the assemblage A isolates were highly genetically homogenous, showing only sub-genotypes A2 (n=3) or A3 (n=13) at the beta-giardin locus and AII only at the gdh locus while assemblage B showed a high genetic polymorphism at both loci. Seven different sub-genotypes were identified within 13 of the sequenced assemblage B beta-giardin isolates. The remaining 13 sequenced assemblage B-isolates appeared to contain several different variants of the beta-giardin gene since the chromatograms displayed one to seven double peaks. The gdh sequences showed an even higher polymorphism since only 2 of 26 assemblage B isolates were without double peaks. Two mixed infections between assemblage A and B were found when the gdh gene was analyzed. Polymorphisms were also observed in the dog-associated assemblages C and D, but to a lesser extent than in assemblage B.
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| 5. |
- Corriols, Marvin, et al.
(författare)
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Geophysical characterization of the Leon-Chinandega aquifer, Nicaragua
- 2008
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Ingår i: Hydrogeology Journal. - : Springer Science and Business Media LLC. - 1431-2174 .- 1435-0157. ; 16:2, s. 349-362
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Tidskriftsartikel (refereegranskat)abstract
- Electrical geophysical surveys in the mode of vertical electrical soundings (VES) and continuous vertical electrical soundings (CVES) were conducted in the Leon-Chinandega plains, northwestern Nicaragua, in order to obtain detailed information about the geometry of the different hydrogeological layers in the aquifer and depth to the basement. A total of 51 VES were carried out within the plains. The results show a complex structure towards the north east of the area, and the southwestern part of the plains presents a smoother stratification. The geoelectrical measurements and borehole information indicate that the basement topography is characterized by hills and deep valleys with highly variable basement depths. Fifty CVES where done in a smaller area in the center of the plain. The resistivity data yielded considerable information revealing the existence of two main geo-electrical units. The combined interpretation of geological and geophysical data shows an environment typical of sedimentary volcanic coastal plains. The information collected during this investigation provides valuable data for estimating the fresh-water resources of the Leon-Chinandega aquifer system and for development of a groundwater management plan.
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| 6. |
- Mendoza, J. A., et al.
(författare)
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Hydrogeological and hydrochemical features of an area polluted by heavy metals in central Nicaragua
- 2006
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Ingår i: Hydrogeology Journal. - : Springer Science and Business Media LLC. - 1431-2174 .- 1435-0157. ; 14:5, s. 777-784
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Tidskriftsartikel (refereegranskat)abstract
- Geophysical and hydrochemical surveys were used to investigate the hydrogeological conditions in one of the Rio Sucio microbasins, in central Nicaragua. Zones of vertical structures (i.e. fractures and quartz veins) and weathering were mapped using Continuous Vertical Electrical Soundings (CVES), as such zones are of major importance for groundwater transport. Water from the springs was analysed to determine concentrations of major ions and heavy metals. Low ion concentrations and 180 analyses indicate that the springs occur close to their recharge areas and there is a relatively rapid groundwater circulation. Mercury (Hg) content in the springs was low, while comparatively high amounts of lead (Pb) were found. The results presented here demonstrate the important function of weathering and tectonics in the occurrence of groundwater systems in the basin. Hg and Pb found in the springs' water reveal the existence of an increase in pollution sources disseminating in the area. More than 100 years of using mercury in the gold-mining industry and releasing wastes into rivers has affected water quality and ecosystems. Further investigations are needed in this area to determine the groundwater vulnerability to this pollution as this resource may be needed in the future.
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| 7. |
- Mendoza, Jose Alfredo, et al.
(författare)
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Aquifer interactions with a polluted mountain river of Nicaragua
- 2008
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Ingår i: Hydrological Processes. - : Wiley. - 1099-1085 .- 0885-6087. ; 22:13, s. 2264-2273
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Tidskriftsartikel (refereegranskat)abstract
- The interactions between a stream and nearby shallow aquifers were investigated in a mountain basin being polluted by mercury released during mining in central Nicaragua. Hourly data series of water levels and temperatures were analysed using cross-correlation. Resistivity imaging was used to map the subsurface and to complement the hydrological data interpretation. The results show the complex hydrogeological conditions that characterize the region, with weathering and fractured rock as main contributors to groundwater transport. The resistivity images suggest the presence of two vertical dykes perpendicular to the stream, and zones rich in clay. The data series indicate a rapid response from the aquifers to recharge events, followed by immediate discharge on a yearly basis. Furthermore, alternating periods of stream infiltration and aquifer discharge were identified. This work demonstrates that surface water pollution is a threat to groundwater quality in the area.
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| 8. |
- Herrera Rodríguez, Andrés, et al.
(författare)
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Pathways to suicidal behaviour among adolescent girls in Nicaragua
- 2006
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Ingår i: Social Science and Medicine. - : Elsevier BV. - 0277-9536 .- 1873-5347. ; 62:4, s. 805-814
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Tidskriftsartikel (refereegranskat)abstract
- Adolescent girls are the most frequent suicide attempters worldwide. However, there is little knowledge about pathways leading to suicidal behaviour among young people, in particular in low-income countries. This study explores the motives and processes related to suicidal behaviour among young girls in Nicaragua. Individual in-depth interviews were conducted with eight girls aged between 12 and 19 admitted to hospital after attempting suicide. The audio-taped interviews lasted 2–4 h and were transcribed, translated into English and coded for content. Grounded theory and content analysis were used to construct a theory of the mechanisms behind their suicidal behaviour. A tentative model exploring pathways to suicidal behaviour is described with four main categories: structuring conditions, triggering events, emotions and actions taken. The model illustrates the dialectic interplay between structure and actions taken. Actions taken were categorized as problem solving or various forms of escape where failure with either of these strategies resulted in a suicide attempt. Dysfunctional families, absent fathers and lack of integration into society were some of the structuring conditions that lead to emotional distress. Abuse, deaths in the family, break-up with boyfriends or suicide among friends acted as triggering events. A striking finding was the obvious narrative competence of the girls. Our findings indicate that suicide prevention programmes for young people must offer support from professionals, independent of their family and social networks. Institutions in the community in contact with young people with suicidal behaviour must develop communicative skills to offer a trusting environment mobilising the resources that young people have.
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| 9. |
- Athanasiadou, Maria, et al.
(författare)
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Polybrominated diphenyl ethers (PBDEs) and bioaccumulative hydroxylated PBDE metabolites in young humans from Managua, Nicaragua.
- 2008
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Ingår i: Environ Health Perspect. - : Environmental Health Perspectives. - 0091-6765 .- 1552-9924. ; 116:3, s. 400-8
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Tidskriftsartikel (refereegranskat)abstract
- OBJECTIVE: Our aim was to investigate exposure to polybrominated diphenyl ethers (PBDEs) in a young urban population in a developing country, with focus on potentially highly exposed children working informally as scrap scavengers at a large municipal waste disposal site. We also set out to investigate whether hydroxylated metabolites, which not hitherto have been found retained in humans, could be detected. METHODS: We assessed PBDEs in pooled serum samples obtained in 2002 from children 11-15 years of age, working and sometimes also living at the municipal waste disposal site in Managua, and in nonworking urban children. The influence of fish consumption was evaluated in the children and in groups of women 15-44 years of age who differed markedly in their fish consumption. Hydroxylated PBDEs were assessed as their methoxylated derivates. The chemical analyses were performed by gas chromatography/mass spectrometry, using authentic reference substances. RESULTS: The children living and working at the waste disposal site showed very high levels of medium brominated diphenyl ethers. The levels observed in the referent children were comparable to contemporary observations in the United States. The exposure pattern was consistent with dust being the dominating source. The children with the highest PBDE levels also had the highest levels of hydroxylated metabolites. CONCLUSIONS: Unexpectedly, very high levels of PBDEs were found in children from an urban area in a developing country. Also, for the first time, hydroxylated PBDE metabolites were found to bioaccumulate in human serum.
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| 10. |
- Bucardo, Filemon, et al.
(författare)
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Pediatric norovirus diarrhea in Nicaragua
- 2008
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Ingår i: Journal of Clinical Microbiology. - 0095-1137 .- 1098-660X. ; 46:8, s. 2573-2580
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Tidskriftsartikel (refereegranskat)abstract
- Information about norovirus (NoV) infections in Central America is limited. Through a passive community and hospital pediatric diarrhea surveillance program, a total of 542 stool samples were collected between March 2005 and February 2006 in León, Nicaragua. NoV was detected in 12% (65/542) of the children; of these, 11% (45/409) were in the community and 15% (20/133) were in the hospital, with most strains (88%) belonging to genogroup II. NoV infections were age and gender associated, with children of <2 years of age (P < 0.05) and girls (P < 0.05) being most affected. Breast-feeding did not reduce the number of NoV infections. An important proportion (57%) of NoV-infected children were coinfected with diarrheagenic Escherichia coli. A significant proportion (18/31) of NoV-positive children with dehydration required intravenous rehydration. Nucleotide sequence analysis (38/65) of the N-terminal and shell region in the capsid gene revealed that at least six genotypes (GI.4, GII.2, GII.4, GII.7, GII.17, and a potentially novel cluster termed "GII.18-Nica") circulated during the study period, with GII.4 virus being predominant (26/38). The majority (20/26) of those GII.4 strains shared high nucleotide homology (99%) with the globally emerging Hunter strain. The mean viral load was approximately 15-fold higher in children infected with GII.4 virus than in those infected with other G.II viruses, with the highest viral load observed for the group of children infected with GII.4 and requiring intravenous rehydration. This study, the first of its type from a Central American country, suggests that NoV is an important etiological agent of acute diarrhea among children of <2 years of age in Nicaragua.
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