| 1. |
- Abdel Ghani, Rania Mahmoud, et al.
(författare)
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A structured evidence-based approach to decrease cardiovascular complications among pregnant women with reumatic disease : a pilot randomized controlled trial
- 2010
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Ingår i: Journal of Medicine and Biomedical Science. - 2078-0273. ; :1, s. 8-15
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Tidskriftsartikel (refereegranskat)abstract
- Background: Recent reports from the developing world have documented rheumatic fever (RE) incidence rates as high as 206/100 000 and Rheumatic Heart Disease (RHD) prevalence rates as high as 18.6/1000. The high frequency of RHD in the developing world necessitates aggressive prevention and control measures. Aim: The aim of the study was to examine the effect of intensive antenatal follow up program on decreasing cardiovascular complications among rheumatic pregnant woman. Design: Randomized controlled trial. Setting: This study was conducted at El Kasr-EL Aini, maternity hospital- Cairo- University Hospitals. Sample: A total of 100 rheumatic pregnant women that attended the antenatal clinic at El Kasr-EL Aini, maternity hospital who could read and write, had functional class I and II cardiac disease, primigravida, nullipara with single fetus were recruited to this study and assigned randomly into two groups. Procedure: Women who met the inclusion criteria were interviewed and baseline assessment was done. The intervention was an intensive antenatal follow up program for decreasing cardiovascular complications among rheumatic pregnant woman. Patients in the study group (SG) were offered an intervention at three levels, basic, intermediate and comprehensive. Evaluation of the program was carried out in term of assessment of maternal and neonatal outcome. Results: There were several significant differences between the study group (SG) and control group (CG). In the SG, antepartum complications was less common, gestational age at birth was higher, postnatal hospital stay shorter, birth weight higher, more had vaginal deliveries with episiotomy, fewer had caesarean section, forceps deliveries and termination of pregnancy, and cardiac class was better at follow up as compared to the CG. Conclusion: The difficult issues in pregnancy complications by cardiac disease are best managed through a team approach. Establishing a pre-pregnancy plan will foster a better therapeutic relationship between all parties and allow for better understanding of the patient’s beliefs regarding pregnancy, her disease and attitude towards medications.
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| 2. |
- Abdel Ghani, Rania Mahmoud, et al.
(författare)
-
A structured evidence-based approach to decrease cardiovascular complications among pregnant women with reumatic disease : a pilot randomized controlled trial
- 2010
-
Ingår i: Journal of Medicine and Biomedical Science. - 2078-0273. ; :1, s. 8-15
-
Tidskriftsartikel (refereegranskat)abstract
- Background: Recent reports from the developing world have documented rheumatic fever (RE) incidence rates as high as 206/100 000 and Rheumatic Heart Disease (RHD) prevalence rates as high as 18.6/1000. The high frequency of RHD in the developing world necessitates aggressive prevention and control measures. Aim: The aim of the study was to examine the effect of intensive antenatal follow up program on decreasing cardiovascular complications among rheumatic pregnant woman. Design: Randomized controlled trial. Setting: This study was conducted at El Kasr-EL Aini, maternity hospital- Cairo- University Hospitals. Sample: A total of 100 rheumatic pregnant women that attended the antenatal clinic at El Kasr-EL Aini, maternity hospital who could read and write, had functional class I and II cardiac disease, primigravida, nullipara with single fetus were recruited to this study and assigned randomly into two groups. Procedure: Women who met the inclusion criteria were interviewed and baseline assessment was done. Theintervention was an intensive antenatal follow up program for decreasing cardiovascular complications among rheumatic pregnant woman. Patients in the study group (SG) were offered an intervention at three levels, basic, intermediate and comprehensive. Evaluation of the program was carried out in term of assessment of maternal and neonatal outcome. Results: There were several significant differences between the study group (SG) and control group (CG). In the SG, antepartum complications was less common, gestational age at birth was higher, postnatal hospital stay shorter, birth weight higher, more had vaginal deliveries with episiotomy, fewer had caesarean section, forceps deliveries and termination of pregnancy, and cardiac class was better at follow up as compared to the CG. Conclusion: The difficult issues in pregnancy complications by cardiac disease are best managed through a team approach. Establishing a pre-pregnancy plan will foster a better therapeutic relationship between all parties and allow for better understanding of the patient’s beliefs regarding pregnancy, her disease and attitude towards medications.
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| 3. |
- Abdel Ghani, Rania Mahmoud, et al.
(författare)
-
Parturient needs during labor : Egyptian women’s perspective toward childbirth experience, a step toward an excellence in clinical practice
- 2011
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Ingår i: Journal of Basic and Applied Scientific Research. - 2090-4304. ; 1:12, s. 2935-2943
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Tidskriftsartikel (refereegranskat)abstract
- Background: Every woman giving birth has expectations. Identifying women’s expectations, wishes, needs and fears enable the health care provider(s) to work toward a common goal of safe and positive childbirth experience. Aim: The aim was to determine women’s preferences and needs during labor. Design: Analytic- cross sectional study. Setting: El Kasr- Aini, - Cairo- University Maternity Hospitals. Sample: A random sample of 400 women were recruited in the present study with the following criteria; age ranged between 20-30 years old, can read and write, primigravida, nulliparous woman with singleton low risk pregnancies, in the third trimester, up to 37 weeks of gestational age, no previous abortion and free from any medical complains. Procedure: Data collection took place in the antenatal clinic, each interview administrated questionnaire took a time between 10-15 minutes. Mothers completed the questionnaire in the antenatal clinics during their waiting for medical examination. Results: Twenty two need requirements emerged from the women's perspective. The highest ranked needs for parturient women during labor are; maintaining privacy through all procedures of 86.5%, accessibility of nurses demonstrate empathy of 67.5%, availability to ventilate and expressing fear and anxiety of 57.5%, quick response to request of 67.5%, frequent monitoring of 52.8%, accessibility of caring medical staff of 47.2% and short delivery of 52.8%. Conclusion: Despite good general coverage of labor care among women, there were clear variations in the type of management given to them or needed by them. This study confirms that different factors predict the multidimensionality of childbirth satisfaction.
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| 4. |
- Abdel Ghani, Rania Mahmoud, et al.
(författare)
-
Parturient needs during labor : Egyptian women’s perspective toward childbirth experience, a step toward an excellence in clinical practice
- 2011
-
Ingår i: Journal of Basic and Applied Scientific Research. - 2090-4304. ; 1:12, s. 2935-2943
-
Tidskriftsartikel (refereegranskat)abstract
- Background: Every woman giving birth has expectations. Identifying women’s expectations, wishes, needs and fears enable the health care provider(s) to work toward a common goal of safe and positive childbirth experience. Aim: The aim was to determine women’s preferences and needs during labor. Design: Analytic- cross sectional study. Setting: El Kasr- Aini, - Cairo- University Maternity Hospitals. Sample: A random sample of 400 women were recruited in the present study with the following criteria; age ranged between 20-30 years old, can read and write, primigravida, nulliparous woman with singleton low risk pregnancies, in the third trimester, up to 37 weeks of gestational age, no previous abortion and free from any medical complains. Procedure: Data collection took place in the antenatal clinic, each interview administrated questionnaire took a time between 10-15 minutes. Mothers completed the questionnaire in the antenatal clinics during their waiting for medical examination. Results: Twenty two need requirements emerged from the women's perspective. The highest ranked needs for parturient women during labor are; maintaining privacy through all procedures of 86.5%, accessibility of nurses demonstrate empathy of 67.5%, availability to ventilate and expressing fear and anxiety of 57.5%, quick response to request of 67.5%, frequent monitoring of 52.8%, accessibility of caring medical staff of 47.2% and short delivery of 52.8%. Conclusion: Despite good general coverage of labor care among women, there were clear variations in the type of management given to them or needed by them. This study confirms that different factors predict the multidimensionality of childbirth satisfaction.
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| 5. |
- Abdel-Khalik, Jonas, et al.
(författare)
-
Incorporation of (14)C-cholesterol in human adrenal corticocarcinoma H295R cell line and online-radiodetection of produced (14)C-steroid hormone metabolites
- 2017
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Ingår i: Journal of Pharmaceutical and Biomedical Analysis. - 0731-7085 .- 1873-264X. ; 145, s. 569-575
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Tidskriftsartikel (refereegranskat)abstract
- This study demonstrates the addition of (14)C-cholesterol to the human cell line H295R will in-situ form radiolabeled steroid hormones allowing for new mechanistic and metabolic insights. The aim of the present study was to in-situ radiolabel steroid hormones from cell line-incorporated (14)C-cholesterol using the OECD guideline 456, H295R steroidogenesis in-vitro assay. Radiodetection of the steroid metabolites of the steroidogenic pathway allows for an improved understanding of the various enzymatic mechanisms involved without necessarily being dependent on quantification. Generated radiolabeled steroids were analyzed using HPLC hyphenated with a Flow Scintillation Analyzer (FSA). H295R cells were incubated with radiolabeled cholesterol and cell media were collected and prepared by solid phase extraction and analyzed with HPLC-FSA. For successful radiolabeling of the steroids in the steroidogenesis of H295R cells, radioactive cholesterol may potentially only need to be added just before the cells are incubated for 72h in well plates. Based on the obtained HPLC-FSA chromatograms, and confirmation of the observations by studies in the literature, a qualitative time profile for the production of steroid hormones was estimated. Multiple radiolabeled steroid hormones were identified by means of analytical standards and UV (ultraviolet) co-chromatography, though the elucidation of multiple metabolites remains unresolved. Although online radiodetection proved to suffer from suboptimal sensitivity, the concept of radiolabeling the steroidogenesis in H295R cells with (14)C-cholesterol and detecting the radiolabeled steroid hormones online was proved and may assist in further toxicological studies.
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| 6. |
- Abdel-Khalik, Jonas, et al.
(författare)
-
Incorporation of (14)C-cholesterol in human adrenal corticocarcinoma H295R cell line and online-radiodetection of produced (14)C-steroid hormone metabolites
- 2017
-
Ingår i: Journal of Pharmaceutical and Biomedical Analysis. - : Elsevier. - 0731-7085 .- 1873-264X. ; 145, s. 569-575
-
Tidskriftsartikel (refereegranskat)abstract
- This study demonstrates the addition of (14)C-cholesterol to the human cell line H295R will in-situ form radiolabeled steroid hormones allowing for new mechanistic and metabolic insights. The aim of the present study was to in-situ radiolabel steroid hormones from cell line-incorporated (14)C-cholesterol using the OECD guideline 456, H295R steroidogenesis in-vitro assay. Radiodetection of the steroid metabolites of the steroidogenic pathway allows for an improved understanding of the various enzymatic mechanisms involved without necessarily being dependent on quantification. Generated radiolabeled steroids were analyzed using HPLC hyphenated with a Flow Scintillation Analyzer (FSA). H295R cells were incubated with radiolabeled cholesterol and cell media were collected and prepared by solid phase extraction and analyzed with HPLC-FSA. For successful radiolabeling of the steroids in the steroidogenesis of H295R cells, radioactive cholesterol may potentially only need to be added just before the cells are incubated for 72h in well plates. Based on the obtained HPLC-FSA chromatograms, and confirmation of the observations by studies in the literature, a qualitative time profile for the production of steroid hormones was estimated. Multiple radiolabeled steroid hormones were identified by means of analytical standards and UV (ultraviolet) co-chromatography, though the elucidation of multiple metabolites remains unresolved. Although online radiodetection proved to suffer from suboptimal sensitivity, the concept of radiolabeling the steroidogenesis in H295R cells with (14)C-cholesterol and detecting the radiolabeled steroid hormones online was proved and may assist in further toxicological studies.
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| 7. |
- Abdel–Khalik, Jonas, et al.
(författare)
-
Development of a solid phase extraction method for the simultaneous determination of steroid hormones in H295R cell line using liquid chromatography–tandem mass spectrometry
- 2013
-
Ingår i: Journal of chromatography. B. - 1570-0232 .- 1873-376X. ; 935:September, s. 61-69
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Tidskriftsartikel (refereegranskat)abstract
- The H295R in vitro cell line produces the majority of the steroidogenesis, for which reason it is commonly used as a screening tool for endocrine disrupting chemicals. Simultaneous determination of the precursor cholesterol and key steroid hormones could give a broad insight into the mechanistic disruption of the steroidogenesis. Steroid hormones have primarily been extracted from H295R incubation medium by means of liquid-liquid extraction (LLE) and the obtained recoveries and matrix effects have typically not been stated or assessed. In the present study a solid-phase extraction (SPE) method was developed and validated for the simultaneous extraction of cholesterol and five key steroid hormones pregnenolone, 17-hydroxyprogesterone, testosterone, cortisol and aldosterone from H295R incubation medium, and finally detected by LC-MS/MS. Cholesterol was recovered at a level of 55.7%, while steroid hormone recoveries ranged from 98.2 to 109.4%. Matrix effects varied between -0.6% and 62.8%. Intra-day precision was deemed acceptable, but the inter-day precision for pregnenolone and aldosterone exceeded the precision limit of 15% RSD. Although LLE has been the most frequently used extraction method in H295R studies, however, our investigation has shown that SPE may relatively easily extract and recover steroid hormones, potentially replacing LLE.
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| 8. |
- Abdel–Khalik, Jonas, et al.
(författare)
-
Development of a solid phase extraction method for the simultaneous determination of steroid hormones in H295R cell line using liquid chromatography–tandem mass spectrometry
- 2013
-
Ingår i: Journal of chromatography. B. - : Elsevier BV. - 1570-0232. ; 935:September, s. 61-69
-
Tidskriftsartikel (refereegranskat)abstract
- The H295R in vitro cell line produces the majority of the steroidogenesis, for which reason it is commonly used as a screening tool for endocrine disrupting chemicals. Simultaneous determination of the precursor cholesterol and key steroid hormones could give a broad insight into the mechanistic disruption of the steroidogenesis. Steroid hormones have primarily been extracted from H295R incubation medium by means of liquid-liquid extraction (LLE) and the obtained recoveries and matrix effects have typically not been stated or assessed. In the present study a solid-phase extraction (SPE) method was developed and validated for the simultaneous extraction of cholesterol and five key steroid hormones pregnenolone, 17-hydroxyprogesterone, testosterone, cortisol and aldosterone from H295R incubation medium, and finally detected by LC-MS/MS. Cholesterol was recovered at a level of 55.7%, while steroid hormone recoveries ranged from 98.2 to 109.4%. Matrix effects varied between -0.6% and 62.8%. Intra-day precision was deemed acceptable, but the inter-day precision for pregnenolone and aldosterone exceeded the precision limit of 15% RSD. Although LLE has been the most frequently used extraction method in H295R studies, however, our investigation has shown that SPE may relatively easily extract and recover steroid hormones, potentially replacing LLE.
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| 9. |
- Abdel–Khalik, Jonas, et al.
(författare)
-
Simultaneous determination of endogenous steroid hormones in human and animal plasma and serum by liquid or gas chromatography coupled to tandem mass spectrometry
- 2013
-
Ingår i: Journal of chromatography. B. - 1570-0232 .- 1873-376X. ; 928:June, s. 59-77
-
Tidskriftsartikel (refereegranskat)abstract
- Analytical methodologies based on liquid or gas chromatography coupled to tandem mass spectrometry for the simultaneous determination of two or more endogenous steroid hormones in human and animal plasma and serum has received increased attention the last few years. Especially in the clinical setting steroid profiling is of major importance in disease diagnostics. This paper discusses recent findings in such multi-steroid hormone procedures published from 2001 to 2012. The aim was to elucidate possible relationships between chosen analytical technique and the obtained analyte sensitivity for endogenous steroid hormones. By evaluating the success, at which the currently applied techniques have been utilized, more general knowledge on the field is provided. Furthermore the evaluation provides directions in which future studies may be interesting to conduct.
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| 10. |
- Abdel–Khalik, Jonas, et al.
(författare)
-
Simultaneous determination of endogenous steroid hormones in human and animal plasma and serum by liquid or gas chromatography coupled to tandem mass spectrometry
- 2013
-
Ingår i: Journal of chromatography. B. - : Elsevier BV. - 1570-0232. ; 928:June, s. 59-77
-
Tidskriftsartikel (refereegranskat)abstract
- Analytical methodologies based on liquid or gas chromatography coupled to tandem mass spectrometry for the simultaneous determination of two or more endogenous steroid hormones in human and animal plasma and serum has received increased attention the last few years. Especially in the clinical setting steroid profiling is of major importance in disease diagnostics. This paper discusses recent findings in such multi-steroid hormone procedures published from 2001 to 2012. The aim was to elucidate possible relationships between chosen analytical technique and the obtained analyte sensitivity for endogenous steroid hormones. By evaluating the success, at which the currently applied techniques have been utilized, more general knowledge on the field is provided. Furthermore the evaluation provides directions in which future studies may be interesting to conduct.
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