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Sökning: WAKA:ovr > Nilsson Mats

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  • Beckman, Claes, Professor, 1962-, et al. (författare)
  • Betänkande av Utredningen om radiospektrumanvändning i framtiden – Frekvenser i samhällets tjänst (SOU 2018:92)
  • 2019
  • Annan publikation (populärvet., debatt m.m.)abstract
    • KTH, har ombetts att ge sin syn på Betänkandet enligt ovan, ert diarienummer N2019/00192/D.Frågeställningarna faller innanför den utbildning och forskning som bedrivs inom KTH. Skolan har under lång tid varit ett nav inom utbildning, utveckling och forskning inom radiokommunikation och även hela system för trådlösa tjänster. Ett exempel är ”Wireless@kth”, som i samarbete med industri och myndigheter bedrivit forskning inom området trådlösa system sedan 2001. Forskare inom denna konstellation har berett frågan och lämnar nedanstående yttrande.
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  • Ciftci, Sibel, 1987-, et al. (författare)
  • Multiplexed rolling circle amplification detection of Ebola, Zika and Dengue towards point-of-care diagnostics
  • Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
    • Emerging tropical viruses have caused serious outbreaks during the recent years, such as Ebola virus (EBOV) in 2014 and the most recent 2018-19 outbreak in Congo. Immediate diagnostic attention is demanded, and most importantly at the point-of-care in resource-limited settings. The performance and the operational parameters of conventional EBOV testing are limited by either their sensitivity, specificity, or both, and often do not cover other tropical disease viruses. We present a padlock probe (PLP)-based rolling circle amplification (RCA) method for the detection of EBOV from cell culture isolates as well as clinical samples obtained from patients of West Africa outbreak. For this, a set of PLPs, separately targeting the vRNA and cRNA of all the seven genes of EBOV, were used in the RCA and validated on virus isolates from cell culture. The assay was then translated for testing clinical samples, and simultaneous duplex detection of both EBOV vRNA and cRNA was demonstrated. For increased sensitivity, the RCA products were enriched on a simple and pump-free microfluidic chip. As PLPs and RCA are inherently mulitplexable, we demonstrate the extension of the probe panel to the simultaneous detection of the tropical viruses Ebola, Zika and Dengue. The simple, rapid, specific and multiplexable isothermal assay developed for tropical virus detection suits the point-of-care needs, bringing RCA a step closer to bedside diagnostics.
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  • Ciftci, Sibel, 1987-, et al. (författare)
  • The sweet detection of rolling circle amplification : Glucose-based electrochemical detection of virus nucleic acid
  • Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
    • Infectious diseases remain a constant threat on a global scale by recurring pandemics. Rapid and portable diagnostics hold the promise to tackle the spreading of diseases and decentralizing healthcare to point-of-care needs. Ebola, a hypervariable RNA virus causing fatalities of up to 90% for recent outbreaks in Africa, demands immediate attention for bedside diagnostics. Nucleic acid amplification technology (NAAT) has proven to be a powerful tool for the control of outbreak with high sensitivity and specificity. However, NAAT is mostly based on amplification methods that require specialized instrumentation and trained personnel, such as PCR with sophisticated detectors. Here, we present an isothermal padlock probe-based assay for the detection of pathogens coupled with a glucose oxidase (GOx)-based electrochemical approach as the read-out. The assay design is based on rolling circle amplification (RCA) upon magnetic beads, connecting the RCA products (RCPs) via streptavidin-biotin bridges to GOx needed for the electrochemical measurement with externally provided glucose. The RCPs forming on the surface of beads are imaged using scanning electron microscopy, and the presence of the GOx to the RCP complex is confirmed using atomic force microscopy. Parameters such as the choice of buffers, concentrations of glucose and GOx and measurement time were optimized, as well as the mode of addition of glucose was tested. 125 μg/mL of GOx with 5 mM glucose using PBS as washing buffer, monitored for 15 min were chosen as the optimized conditions. The effect of temperature was tested and found to be critical at 37 °C for enhanced performance of the sensor. Finally, we evaluate the analytical performance of our sensor system by using cell culture isolate and clinical samples of Ebola virus. The study provides a proof-of-concept of simple and portable molecular diagnostics for emerging pathogens, beneficial especially for resource-limited settings. 
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  • Cornell Kärnekull, Stina, 1985-, et al. (författare)
  • The reminiscence bump is blind to blindness : Evidence from sound- and odor-evoked autobiographical memory
  • Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
    • Autobiographical memories (AMs) evoked by sensory cues, such as words, pictures, and sounds, typically form reminiscence bumps in adolescence and young adulthood. However, odors constitute an exception by shifting the bump to early childhood. Olfaction may be a “sense of first impressions”, as indicated by a unique hippocampal representation in the brain for first odor-to-object associations. However, the influence of the individual’s sensory function on AMs has never been examined. We examined the reminiscence bumps of sound- and odor-evoked memories of early-blind and sighted individuals, since blindness implies considerable changes in sensory experience. Despite such changes, the groups displayed similar age distributions of both sound- and odor-evoked memories. The auditory bump seemed to span the first two decades of life, whereas the olfactory bump was once again found in early childhood. Hence, the reminiscence bumps were robust to differences in sensory function and experience.
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