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- Fan, Yanmiao, et al.
(författare)
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Detection of gemcitabine metabolism using 19F-NMR and its impacts on E. coli morphology
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- 19F-NMR spectroscopy is a sensitive analytical method to detect the metabolism of fluorine-containing drugs by bacteria. In this study, 19F-NMR was used to achieve the real time detection of metabolic process of gemcitabine (2′, 2′-difluorodeoxycytidine) by Escherichia Coli (E. coli) in the nutrient broth. Both E. coli and Staphylococcus aureus (S. aureus) were used in the metabolism study. E. coli can metabolize gemcitabine, while gemcitabine cannot be metabolized by S. aureus. Our results showed that gemcitabine can be totally metabolized to its inactive form 2′, 2′-difluorodeoxyuridine (dFdU) by E. coli both in Mueller-Hinton broth and M9 minimal salt. The metabolic rate of gemcitabine has a positive correlation with the bacterial concentrations. The metabolism is due to the presence of bacterial cytidine deaminase, and the enzyme inhibitor tetrahydrouridine (THU) can inhibit the gemcitabine metabolism. Scanning electron microscope (SEM) was used to study the effects of gemcitabine metabolism on E. coli morphological changes, and the treated E. coli was 2-3 times longer than the normal bacteria. 19F–NMR was capable to achieve real time detection of gemcitabine metabolism process considering there was no need to separate the bacterial cells from the nutrient medium, this study provided a fast and facile way to detect fluorine-containing drug metabolism by bacteria.
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- Fan, Yanmiao, et al.
(författare)
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Fluorescent silicon nanoparticles as a potential capaturing and imaging agent for Staphylococcus aureus
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- Nanomaterials, with various shapes and specific physicochemical properties, have attracted a lot of interest in the biomedical fields. Fluorescent silicon nanoparticles (SiNPs) have shown promise as immunofluorescent cellular imaging agents. In this study, SiNPs were synthesized to explore their potential as bacterial imaging agents. Silicon nanoparticles with amino groups on the surface were prepared using microwave-assisted synthesis method using trisodium citrate as the reducing agent. The obtained SiNPs were characterized using dynamic light scattering (DLS), UV−vis absorption spectroscopy and fluorescence spectrophotometer. And SiNPs were used to study their interactions with both Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus). The interactions between the prepared SiNPs and bacteria were monitored by the fluorescence microscope, and SiNPs showed a strong interaction with S. aureus. SEM was used to study the morphological changes of S. aureus, and we found that the cell membranes of S. aureus became damaged after the interaction with SiNPs. The amino groups on the surfaces of SiNPs could also be functionalized with other functional groups for other applications.
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- Karalius, Antanas, et al.
(författare)
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Control Over Emergent π-π-Interactions in Double-Dynamic Coordination Complexes Through a Nature-Inspired Coordination-Triggered System
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- A complex self-assembly system based on coordination of dynamic imine/hemiacetal ligands isdemonstrated. Natural dipicolinic acid complexes are considered intercalants of certain bacterialDNA and implicated in thermal resistance of the dormant cell, and 6-formyl-2-picolinate was usedas building block. The dipicolinate-mimicking system showed an emergent and preferentialdynamic, drawbridge-style, π-π interaction effect to the opposing meridional ligand in octahedralcomplexes. The interactions proved to be influenced by the ligand net charge, the bridging arylgroup substitution, and the coordinating metal charge. The application scope of the effect isdiscussed.
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