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Sökning: WAKA:ref > Inganäs Olle > Hammarström Per > Refereegranskat > Linköpings universitet > (2005) > Engelska

  • Resultat 1-3 av 3
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  • Herland, Anna, et al. (författare)
  • Synthesis of a regioregular zwitterionic conjugated oligoelectrolyte, usable as an optical probe for detection of amyloid fibril formation at acidic pH
  • 2005
  • Ingår i: Journal of the American Chemical Society. - 0002-7863. ; 127:7, s. 2317-2323
  • Tidskriftsartikel (refereegranskat)abstract
    • Changes of the optical properties of conjugated polyelectrolytes have been utilized to monitor noncovalent interactions between biomolecules and the conjugated polyelectrolytes in sensor applications. A regioregular, zwitterionic conjugated oligoelectrolyte was synthesized in order to create a probe with a defined set of optical properties and hereby facilitate interpretation of biomolecule−oligoelectrolyte interactions. The synthesized oligoelectrolyte was used at acidic pH as a novel optical probe to detect amyloid fibril formation of bovine insulin and chicken lysozyme. Interaction of the probe with formed amyloid fibrils results in changes of the geometry and the electronic structure of the oligoelectrolyte chains, which were monitored with absorption and emission spectroscopy.
  • Nilsson, Peter, et al. (författare)
  • Conjugated polyelectrolytes : conformation-sensitive optical probes for detection of amyloid fibril formation
  • 2005
  • Ingår i: Biochemistry. - 0006-2960. ; 44:10, s. 3718-3724
  • Tidskriftsartikel (refereegranskat)abstract
    • The in vivo deposition of amyloid fibrils is a hallmark of many devastating diseases known as the amyloidoses. Amyloid formation in vitro may also complicate production of proteins in the biotechnology industry. Simple, sensitive, and versatile tools that detect the fibrillar conformation of amyloidogenic proteins are thus of great importance. We have developed a negatively charged conjugated polyelectrolyte that displays different characteristic optical changes, detected visually or by absorption and emission, depending on whether the protein with which it forms a complex is in its native state or amyloid fibril conformation. This simple, rapid, and novel methodology was applied here to two amyloidogenic proteins, insulin and lysozyme, and its validity for detection of their fibrillar conformation was verified by currently used methods such as circular dichroism, transmission electron microscopy, and Congo red absorption.
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  • Resultat 1-3 av 3
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tidskriftsartikel (3)
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Herland, Anna, (3)
Konradsson, Peter, (2)
Nilsson, K. Peter R. ... (2)
Nilsson, Peter, (1)
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Björk, Per (1)
Olsson, Johan D. M., (1)
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