| 1. |
- Hill, Daniel, et al.
(författare)
-
Microfluidic and Transducer Technologies for Lab on a Chip Applications
- 2010
-
Ingår i: 2010 ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY (EMBC). - : IEEE conference proceedings. - 9781424441242 ; , s. 305-307
-
Konferensbidrag (refereegranskat)abstract
- Point-of-care diagnostic devices typically require six distinct qualities: they must deliver at least the same sensitivity and selectivity, and for a cost per assay no greater than that of today's central lab technologies, deliver results in a short period of time (<15 min at GP; <2h in hospital), be portable or at least small in scale, and require no or extremely little sample preparation. State-of-the-art devices deliver information of several markers in the same measurement.
|
|
| 2. |
|
|
| 3. |
|
|
| 4. |
|
|
| 5. |
- Banerjee, Indradumna, 1986-, et al.
(författare)
-
LDH based neonatal diagnostics on a low-cost slipdisc based sample preparation platform.
- 2016
-
Konferensbidrag (refereegranskat)abstract
- INTRODUCTIONSlipdisc is developed as a sample preparation platform based on slipchip technology [1], using a handwinded clockwork mechanism allowing sample processing from one spot to another with defined precision without the need for sophisticated tools or alignment (Fig.1). An ordinary smartphone or camera can be used to image and analyse the results making it an ideal tool for resource limited settings. Here, we demonstrate a bioassay for detecting LDH (Fig.2), a crucial enzyme found in all living cells which leaks out when the cellular membrane is damaged. This makes LDH a biomarker for several medical conditions in newborns, such as Ozkiraz-13, necrotizing enterocolitis (NEC), and Asphyxia.EXPERIMENTALFor assembling the slipdisc optically transparent, robust and disposable CD like polycarbonate discs were used with superhydrophobic coating on all except the embedded microfluidic channels. For the LDH assay, heparinized plasma samples were spiked with 7 different concentrations of the LDH enzyme (Lee Biosolutions, USA). These concentrations ranged from clinically normal to abnormal concentrations and used to construct a standard curve for LDH enzyme.RESULTS AND DISCUSSIONThe ability of the SlipDisc to quantify LDH enzyme levels from plasma samples was evaluated (Fig.3). Using 7 different concentrations, a standard curve with clinically relevant LDH concentrations was obtained (Fig4). Image and data analyses, including linear regression and Pearson’s correlation, were completed using Image processing tool in Matlab.CONCLUSIONWe demonstrate a low-cost neonatal diagnostics platform for the detection of LDH from plasma using a novel SlipDisc platform. The SlipDisc can further be modified to separate plasma from whole blood samples in order to fully integrate the assay. Its simple operation and smartphone based detection capabilities make it an ideal device for point-of-care neonatal diagnostics.
|
|
| 6. |
|
|
| 7. |
- Hansson, Jonas, et al.
(författare)
-
Inertial Particle Focusing In Parallel Microfluidic Channels For High-Throughput Filtration
- 2011
-
Ingår i: 16th International Solid-State Sensors, Actuators and Microsystems Conference (TRANSDUCERS), 2011. - : IEEE conference proceedings. - 9781457701573 ; , s. 1777-1780
-
Konferensbidrag (refereegranskat)abstract
- In this study, we introduce inertial microfluidics in straight, parallel channels for high-throughput particle filtration. We show that particles flowing through low aspect ratio rectangular microchannels can be focused into four particle streams, distributed at the centers of each wall face, or into two particle streams, at the centers of the longest channel walls, depending on the particles' size. For high-throughput filtration, we fabricated scalable, single inlet and two outlet, parallel channel microdevices, using a high-density 3D microfluidic PDMS channel manufacturing technology, in a design that allows for easy integration with other downstream on-chip functions we recently described. We demonstrate filtration of 24 μm particles from a suspension mixture in a microdevice with four parallel channels. The filtration efficiency at a non-optimized flow rate of 0.8 ml/min was 82%.
|
|
| 8. |
- Jayne, David R W, et al.
(författare)
-
Glomerulonephritides.
- 2014
-
Ingår i: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association. - : Oxford University Press (OUP). - 1460-2385. ; 29 Suppl 3, s. 27-29
-
Konferensbidrag (refereegranskat)
|
|
| 9. |
- Karlsson, J. Mikael, et al.
(författare)
-
On-Chip Liquid Degassing With Low Water Loss
- 2010
-
Ingår i: Proceedings Micro Total Analysis Systems (μTAS) 2010. - Groningen : CBMS. - 9780979806438 ; , s. 1790-1792
-
Konferensbidrag (refereegranskat)abstract
- We present a novel approach for actively degassing liquid and removing trapped air bubbles in microfluidic devices.In our approach, an integrated gas permeable membrane, consisting of a structurally supporting PDMS layer that is covered with a thin Teflon® AF 1600 film, separates the on-chip liquid from an on-chip low-vacuum chamber. Since the Teflon AF permeability is near zero for liquid water and low for vapour, air bubbles and dissolved air are removed through the membrane whilst the loss of water, ions and biomolecules in the system remains low. The system has been demonstrated at elevated temperatures and could be suitable for e.g. degassing during on-chip PCR.
|
|
| 10. |
- Lundgren, Stina, et al.
(författare)
-
Micro reactors for the optimisation of reaction conditions in asymmetric metal catalysis
- 2005
-
Ingår i: Micro Total Analysis Systems 2004. - 0854046437 ; , s. 445-447
-
Konferensbidrag (refereegranskat)abstract
- Two types of micro reactors were employed for enantioselective metal catalysed reactions. In the first type of reactor, an electroosmotic flow was used, whereas the second type of reactor used a pressure driven flow. The purpose of the study is to develop tools for rapid and efficient optimization of reactions, utilising minimum amounts of reagents.
|
|
