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Träfflista för sökning "WFRF:(Al Haidari Amr A.) "

Sökning: WFRF:(Al Haidari Amr A.)

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1.
  • Al-Haidari, Amr A., et al. (författare)
  • MiR-155-5p positively regulates CCL17-induced colon cancer cell migration by targeting RhoA
  • 2017
  • Ingår i: Oncotarget. - Impact Journals, LLC. - 1949-2553. ; 8:9, s. 14887-14896
  • Tidskriftsartikel (refereegranskat)abstract
    • Colorectal cancer is the second most common cause of cancer-related death, which is due to migration of tumor cells to distant sites of metastasis. Accumulating data indicate that mciroRNAs play an important role in several aspects of colon cancer cell biology. Herein, we examined the role of miR-155-5p in colon cancer cell migration induced by the CCL17-CCR4 axis in HT-29 colon cancer cells. We found that miR-155-5p knockdown in serum starved colon cancer cells decreased CCL17-induced cell chemotaxis. Moreover, knocking down miR-155-5p markedly decreased CCL17-provoked activation of RhoA in colon cancer cells. Bioinformatics analysis predicted two putative binding sites in the AU-rich element at the 3'-UTR of RhoA mRNA. MiR-155-5p binding to RhoA mRNA was verified using a target site blocker and functionally validated by RNA immunoprecipitation assays, showing that miR-155-5p-dependent regulation of RhoA mRNA is mediated by AU-rich elements present in the 3'-UTR region. Taken together, these results show that miR-155-5p positively regulates RhoA mRNA levels and translation as well as cell migration in serum starved colon cancer cells and indicate that targeting miR-155-5p might be a useful strategy to antagonize colon cancer metastasis.
2.
  • Al-Haidari, Amr A., et al. (författare)
  • Neutrophil extracellular traps promote peritoneal metastasis of colon cancer cells
  • 2019
  • Ingår i: Oncotarget. - Impact Journals, LLC. - 1949-2553. ; 10:12, s. 1238-1249
  • Tidskriftsartikel (refereegranskat)abstract
    • Cytoreductive surgery is the only curative option for patients with peritoneal carcinomatosis, however, intraperitoneal recurrence rate is high making new ways to prevent cancer recurrence an urgent need. Recent evidence suggests that neutrophils are involved in cancer progression. The purpose of our study was to examine the role of neutrophils in the spread of colon cancer cells in the peritoneal cavity. The number of metastatic noduli in the peritoneal cavity was quantified in mice injected with murine colon cancer cells (CT-26) intraperitoneally after surgical laparotomy and treated with a neutrophil depleting antibody or DNase I. In addition, peritoneal metastases were harvested from patients with peritoneal carcinomatosis. Scanning and transmission electron microscopy showed extensive neutrophil extracellular trap (NET) formation in peritoneal colon cancer metastases in mice and patients. Neutrophil depletion markedly reduced the number of metastases in laparotomised animals. Administration of DNase I decreased the number of metastatic nodules by 88% in laparotomised animals as well as NET-induced chemokinedependent colon cancer cell migration and adhesion in vitro. Finally, CT-26 cancer cells were found to express the avβ3 integrin and inhibition of av integrin abolished NET-induced adhesion of colon cancer cells to vitronectin. Taken together, our data show that NETs play an important role in colon cancer cell metastasis in the peritoneal cavity and regulate colon cancer cell migration and adhesion to extracellular matrix proteins. These novel findings suggest that targeting NETs might be an effective strategy to antagonize intrabdominal recurrences of colon cancer after cytoreductive surgery in patients with peritoneal carcinomatosis.
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  • Resultat 1-2 av 2
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fritt online (2)
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tidskriftsartikel (2)
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refereegranskat (2)
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Thorlacius, Henrik, (2)
Syk, Ingvar, (2)
Al-Haidari, Amr A., (2)
Rahman, Milladur, (1)
Algethami, Nader, (1)
Lepsenyi, Mattias, (1)
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Lunds universitet (2)
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Engelska (2)
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Medicin och hälsovetenskap (2)

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