| 1. |
- Micah, Angela E., et al.
(författare)
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Tracking development assistance for health and for COVID-19 : a review of development assistance, government, out-of-pocket, and other private spending on health for 204 countries and territories, 1990-2050
- 2021
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Ingår i: The Lancet. - : Elsevier. - 0140-6736 .- 1474-547X. ; 398:10308, s. 1317-1343
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Forskningsöversikt (refereegranskat)abstract
- Background The rapid spread of COVID-19 renewed the focus on how health systems across the globe are financed, especially during public health emergencies. Development assistance is an important source of health financing in many low-income countries, yet little is known about how much of this funding was disbursed for COVID-19. We aimed to put development assistance for health for COVID-19 in the context of broader trends in global health financing, and to estimate total health spending from 1995 to 2050 and development assistance for COVID-19 in 2020. Methods We estimated domestic health spending and development assistance for health to generate total health-sector spending estimates for 204 countries and territories. We leveraged data from the WHO Global Health Expenditure Database to produce estimates of domestic health spending. To generate estimates for development assistance for health, we relied on project-level disbursement data from the major international development agencies' online databases and annual financial statements and reports for information on income sources. To adjust our estimates for 2020 to include disbursements related to COVID-19, we extracted project data on commitments and disbursements from a broader set of databases (because not all of the data sources used to estimate the historical series extend to 2020), including the UN Office of Humanitarian Assistance Financial Tracking Service and the International Aid Transparency Initiative. We reported all the historic and future spending estimates in inflation-adjusted 2020 US$, 2020 US$ per capita, purchasing-power parity-adjusted US$ per capita, and as a proportion of gross domestic product. We used various models to generate future health spending to 2050. Findings In 2019, health spending globally reached $8. 8 trillion (95% uncertainty interval [UI] 8.7-8.8) or $1132 (1119-1143) per person. Spending on health varied within and across income groups and geographical regions. Of this total, $40.4 billion (0.5%, 95% UI 0.5-0.5) was development assistance for health provided to low-income and middle-income countries, which made up 24.6% (UI 24.0-25.1) of total spending in low-income countries. We estimate that $54.8 billion in development assistance for health was disbursed in 2020. Of this, $13.7 billion was targeted toward the COVID-19 health response. $12.3 billion was newly committed and $1.4 billion was repurposed from existing health projects. $3.1 billion (22.4%) of the funds focused on country-level coordination and $2.4 billion (17.9%) was for supply chain and logistics. Only $714.4 million (7.7%) of COVID-19 development assistance for health went to Latin America, despite this region reporting 34.3% of total recorded COVID-19 deaths in low-income or middle-income countries in 2020. Spending on health is expected to rise to $1519 (1448-1591) per person in 2050, although spending across countries is expected to remain varied. Interpretation Global health spending is expected to continue to grow, but remain unequally distributed between countries. We estimate that development organisations substantially increased the amount of development assistance for health provided in 2020. Continued efforts are needed to raise sufficient resources to mitigate the pandemic for the most vulnerable, and to help curtail the pandemic for all. Copyright (C) 2021 The Author(s). Published by Elsevier Ltd.
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| 2. |
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- 2019
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Tidskriftsartikel (refereegranskat)
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| 3. |
- Kanai, M, et al.
(författare)
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- 2023
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swepub:Mat__t
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| 4. |
- Kazmi, Bilal, et al.
(författare)
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Thermodynamic and economic assessment of cyano functionalized anion based ionic liquid for CO2 removal from natural gas integrated with, single mixed refrigerant liquefaction process for clean energy
- 2022
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Ingår i: Energy. - : Pergamon Press. - 0360-5442 .- 1873-6785. ; 239
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Tidskriftsartikel (refereegranskat)abstract
- The study proposes a novel integrated process in which ionic liquid is utilized to control carbon dioxide (CO2) emissions from the natural gas combined with a single mixed refrigerant-based liquefaction process to assist safe transportation over long distances providing a sustainable and cleaner energy. Commercially amines are utilized for CO2 sequestration, but amines entail energy-intensive regeneration with elevated process costs. The present study offers a solvent screening mechanism based on important parameters such as heat of dissolution, viscosity, selectivity, working capacity, vapor pressure, corrosivity, and toxicity. The selected solvents' performance is computed by sensitivity analysis suggesting imidazolium-based cation 1-hexyl-3-methylimidazolium[Hmim] functionalized with tricyanomethanide(tcm) as anion a potential natural gas sweetening solvent in comparison with commercially used solvent monoethanoloamine(MEA), conventional ILs 1-butyl-3-methylimidazolium hexa-fluorophosphate [Bmim][Pf(6)] and 1-butyl-3-methylimidazolium methyl sulfate [Bmim][MeSO4]. The obtained sweet gas is liquefied using a single mixed refrigerant-based process providing 0.99 mol fraction of liquefied CH4 with less overall specific compression power requirement of 0.41 kW/kg of natural gas. Moreover, an exergy analysis demonstrates that the [Hmim][tcm] based process has lower total exergy destruction of 7.49 x 10(3) kW and is found to utilize less overall specific energy consumption 0.49 kWh/kg of NG in contrast to other studied solvents. Furthermore, a detailed economic analysis establishes [Hmim][tcm]-based CO2 integrated with liquefaction technology offers 50.7%, 74.4%, and 85.8% of total annualized cost (TAC) savings compared with the MEA-amim][Pf(6)]-, and [Bmim][MeSO4], respectively. Hence, [Hmim][tcm] for CO2 removal and integration with liquefaction process will incur unit cost based on the total annualized cost to be $2.2 x 10(4)/kmol of purified NG.
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| 5. |
- Ali, N., et al.
(författare)
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Antibacterial and antifungal activity of solvent extracts from Plumeria obtusa Linn
- 2014
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Ingår i: Tropical Biomedicine. - 0127-5720. ; 31:4, s. 607-615
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Tidskriftsartikel (refereegranskat)abstract
- Extracts of Plumeria obtusa are widely used in ethnomedicine and have been investigated for a variety of biological activities; however, the antimicrobial activity of P. obtusa flowers is poorly characterized. In this study, the antimicrobial activities of different solvents (petroleum ether, ethyl acetate, chloroform, isobutanol and ethanol) extracts from flowers of P. obtusa were investigated by a disc diffusion method against Gram-positive bacteria, Gram-negative bacteria and a fungus. All extracts exhibited growth inhibition of all microorganisms at variable degrees as measured by relative zones of inhibition, however, the petroleum ether extract was ineffective against Klebsiella pneumonia and ethyl acetate and isobutanol extracts were ineffective against Pseudomonas aeruginosa. The most susceptible Gram-positive bacterium was Bacillus subtilis while the most resistant Gram-positive bacterium was Staphylococcus aureus. Erwinia carotovora was the most susceptible Gram-negative bacterium while P. aeruginosa was highly resistant among the Gram-negative bacteria. In this study, for the first time, we investigated the antimicrobial activity of several different solvent extracts from flowers of P. obtusa against a broad spectrum of human-pathogenic microorganisms. These compounds warrant further investigation by isolation and structural elucidation with the aim to find novel and affordable bioactive compounds for the treatment of infectious diseases.
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| 6. |
- Junaid, M., et al.
(författare)
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A straightforward experimental approach to expression, purification, refolding, and enzymatic analysis of recombinant dengue virus NS2B(H)-NS3pro protease
- 2013
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Ingår i: Biochemistry (Moscow). - 0006-2979 .- 1608-3040. ; 78:8, s. 920-924
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Tidskriftsartikel (refereegranskat)abstract
- Dengue virus threatens around 2.5 billion people worldwide; about 50 million become infected every year, and yet no vaccine or drug is available for prevention and/or treatment. The flaviviral NS2B-NS3pro complex is indispensable for flaviviral replication and is considered to be an important drug target. The aim of this study was to develop a simple and generally applicable experimental strategy to construct, purify, and assay a highly active recombinant NS2B(H)-NS3pro complex that would be useful for high-throughput screening of potential inhibitors. The sequence of NS2B(H)-NS3pro was generated by overlap extension PCR (SOE-PCR) and cloned into the pTrcHisA vector. Hexahistidine-tagged NS2B(H)-NS3pro complex was expressed in E. coli predominantly as insoluble protein and purified to > 95% purity by single-step immobilized metal affinity chromatography. SDS-PAGE followed by immunoblotting of the purified enzyme demonstrated the presence of the NS2B(H)-NS3pro precursor and its autocleavage products, NS3pro and NS2B(H), as 37, 21, and 10 kDa bands, respectively. Kinetic parameters, K (m), k (cat), and k (cat)/K (m) for the fluorophore-linked protease model substrate Ac-nKRR-amc were obtained using inner-filter effect correction. The kinetic parameters K (m), k (cat), and k (cat)/K (m) for Ac-nKRR-amc substrate were 100 mu M, 0.112 s(-1), and 1120 M-1 center dot s(-1), respectively. A simplified procedure for the cloning, overexpression, and purification of the NS2B(H)-NS3pro complex was applied, and a highly active recombinant NS2B(H)-NS3pro complex was obtained that could be useful for the design of high-throughput assays aimed at flaviviral inhibitor discovery.
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| 7. |
- Junaid, M., et al.
(författare)
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Modulation of enzymatic activity of dengue virus nonstructural protein NS3 nucleoside triphosphatase/helicase by poly(U)
- 2013
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Ingår i: Biochemistry (Moscow). - 0006-2979 .- 1608-3040. ; 78:8, s. 925-932
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Tidskriftsartikel (refereegranskat)abstract
- The nonstructural protein 3 (NS3) appears to be the most promising target for anti-flavivirus therapy because of its multiple enzymatic activities that are indispensable for virus replication. NS3 of dengue virus type 2 (DEN2) is composed of two domains, a serine protease in the N-terminal domain (NS3pro) and RNA-stimulated nucleoside triphosphatase (NTPase)/RNA helicase at the C-terminus (NS3h). NS3 plays an important role in viral replication and the coordinated regulation of all the catalytic activities in the full-length NS3 protein. In this study, a plasmid harboring the NS3 helicase domain (NS3h) was constructed by PCR. The 56.5 kDa NS3h protein was purified by metal-chelate affinity chromatography followed by renaturation, mediated by artificial chaperone-assisted refolding, which yielded the active helicase. NTPase activity was assayed with Malachite Green. The NTPase activity in the presence of poly(U) showed a higher turnover number (k (cat)) and a lower K (m) value than without poly(U). The activity increased approximately fourfold in the presence of polynucleotides. This indicates that NTPase activity of dengue NS3 can be stimulated by polynucleotides. A helicase assay based on internal fluorescence quenching was conducted using short internally quenched DNA oligonucleotides as substrates. Significant fluorescence signaling increase was observed in the absence of polynucleotides such as poly(U). No unwinding activity was observed with addition of poly(U). The approach we describe here is useful for the further characterization of substrate specificity and for the design of high-throughput assays aimed at discovery of inhibitors against NS3 NTPase/helicase activities.
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| 8. |
- Khan, Noman, et al.
(författare)
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Dysregulation of metalloproteins in ischemic heart disease patients with systolic dysfunction
- 2023
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Ingår i: International Journal of Biological Macromolecules. - : Elsevier. - 0141-8130 .- 1879-0003. ; 232
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Tidskriftsartikel (refereegranskat)abstract
- Ischemic heart disease (IHD) is the leading cause of mortality worldwide. Metalloproteins have been linked to human health and diseases. The molecular functions of metalloproteins in IHD is not well understood and require further exploration. The objective of this study was to find out the role of metalloproteins in the pericardial fluid of IHD patients having normal (EF > 45) and impaired (EF < 45) left ventricular ejection fraction (LVEF). IHD patients were grouped into two categories: LVEF<45 (n = 12) and LVEF >45 (n = 33). Pooled samples of pericardial fluid were fractionated by using ZOOM-isoelectric focusing (IEF) followed by further processing using one-dimensional gel electrophoresis (1D SDS-PAGE) and filter-aided sample preparation (FASP). Tryptic peptides of each fraction and differential bands were then analyzed by nano-LC-ESI-MS/MS. Protein identification was performed through a Mascot search engine using NCBI-Prot and SwissProt databases. A total of 1082 proteins including 154 metalloproteins were identified. In the differential bands, 60 metalloproteins were identified, while 115 metalloproteins were identified in all ZOOM-IEF fractions. Twelve differentially expressed metalloproteins were selected in the intense bands according to their molecular weight (MW) and isoelectric point (pI). The 12 differentially expressed metalloprotein includes ceruloplasmin, Prothrombin, Vitamin K-dependent protein, Fibulin-1, Ribosomal protein S6 kinase alpha-6, nidogen, partial, Serum albumin, Hemopexin, C-reactive protein, Serum amyloid P-component, and Intelectin-1 protein which were all up-regulated while serotransferrin is the only metalloprotein that was down-regulated in impaired (LVEF<45) group. Among the metalloproteins, Zn-binding proteins are 36.5 % followed by Ca-binging 32.2 %, and Fe-binging 12.2 %. KEGG, pathway analysis revealed the association of ceruloplasmin and serotransferrin with the ferroptosis pathway. In conclusion, 154 metalloproteins were identified of them the Zn-binding protein followed by Ca-binding and Fe-binding proteins were the most abundant metalloproteins. The two metalloproteins, the Cu-binding protein ceruloplasmin, and Fe-binding protein serotransferrin are involved in the ferroptosis pathway, an iron-dependent form of regulated cell death that has been linked to cardiac pathology, especially in IHD patients having impaired systolic (LVEF<45) dysfunction. However, further research is required to validate these findings.
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| 9. |
- Mehboob, Usama, et al.
(författare)
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Genetic algorithms in wireless networking : techniques, applications, and issues
- 2016
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Ingår i: Soft Computing - A Fusion of Foundations, Methodologies and Applications. - : Springer Science and Business Media LLC. - 1432-7643 .- 1433-7479. ; 20:6, s. 2467-2501
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Tidskriftsartikel (refereegranskat)abstract
- In recent times, wireless access technology is becoming increasingly commonplace due to the ease of operation and installation of untethered wireless media. The design of wireless networking is challenging due to the highly dynamic environmental condition that makes parameter optimization a complex task. Due to the dynamic, and often unknown, operating conditions, modern wireless networking standards increasingly rely on machine learning and artificial intelligence algorithms. Genetic algorithms (GAs) provide a well-established framework for implementing artificial intelligence tasks such as classification, learning, and optimization. GAs are well known for their remarkable generality and versatility and have been applied in a wide variety of settings in wireless networks. In this paper, we provide a comprehensive survey of the applications of GAs in wireless networks. We provide both an exposition of common GA models and configuration and provide a broad-ranging survey of GA techniques in wireless networks. We also point out open research issues and define potential future work. While various surveys on GAs exist in the literature, our paper is the first paper, to the best of our knowledge, which focuses on their application in wireless networks.
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| 10. |
- Mohamed, Ali, et al.
(författare)
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Enhancing Cyber Security of LoRaWAN Gateways under Adversarial Attacks
- 2022
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Ingår i: Sensors. - : MDPI AG. - 1424-8220. ; 22:9
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Tidskriftsartikel (refereegranskat)abstract
- The Internet of Things (IoT) has disrupted the IT landscape drastically, and Long Range Wide Area Network (LoRaWAN) is one specification that enables these IoT devices to have access to the Internet. Former security analyses have suggested that the gateways in LoRaWAN in their current state are susceptible to a wide variety of malicious attacks, which can be notoriously difficult to mitigate since gateways are seen as obedient relays by design. These attacks, if not addressed, can cause malfunctions and loss of efficiency in the network traffic. As a solution to this unique problem, this paper presents a novel certificate authentication technique that enhances the cyber security of gateways in the LoRaWAN network. The proposed technique considers a public key infrastructure (PKI) solution that considers a two-tier certificate authority (CA) setup, such as a root-CA and intermediate-CA. This solution is promising, as the simulation results validate that about 66.67% of the packets that are arriving from an illegitimate gateway (GW) are discarded in our implemented secure and reliable solution.
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