| 1. |
- Andersen, Kristina, 1970-, et al.
(författare)
-
The Dial: Exploring Computational Strangeness
- 2016
-
Ingår i: Proceedings of the 2016 CHI Conference Extended Abstracts on Human Factors in Computing Systems (CHI EA '16). - New York, USA : ACM Press.
-
Konferensbidrag (refereegranskat)abstract
- This paper describes the process of a computational ideaemerging from a process of user engagement: algorithmicrecommendations as artistic obstructions in creativework. Through a collaboration between HCI and Music InformationRetrieval, we conducted open-ended interviewswith professional makers of Electronic Dance Music. Wedescribe how the idea emerged from this process, and considerhow algorithmic recommendation systems could bere-considered as tools for artistic inspiration. We proposethe concept of a “Strangeness Dial,” which allows the gradualadjustment of the degree of desired otherness, which istested through the use of a non-functional prop and a seriesof interviews.
|
|
| 2. |
- Bergemalm, Daniel, et al.
(författare)
-
Superoxide dismutase-1 and other proteins in inclusions from transgenic amyotrophic lateral sclerosis model mice
- 2010
-
Ingår i: Journal of Neurochemistry. - : Wiley. - 0022-3042 .- 1471-4159. ; 114:2, s. 408-418
-
Tidskriftsartikel (refereegranskat)abstract
- Mutant superoxide dismutase-1 (SOD1) causes amyotrophic lateral sclerosis (ALS) through a cytotoxic mechanism of unknown nature. A hallmark in ALS patients and transgenic mouse models carrying human SOD1 (hSOD1) mutations are hSOD1-immunoreactive inclusions in spinal cord ventral horns. The hSOD1 inclusions may block essential cellular functions or cause toxicity through sequestering of other proteins. Inclusions from four different transgenic mouse models were examined after density gradient ultracentrifugation. The inclusions are complex structures with heterogeneous densities and are disrupted by detergents. The aggregated hSOD1 was mainly composed of subunits that lacked the native stabilizing intra-subunit disulfide bond. A proportion of subunits formed hSOD1 oligomers or was bound to other proteins through disulfide bonds. Dense inclusions could be isolated and the protein composition was analyzed using proteomic techniques. Mutant hSOD1 accounted for half of the protein. Ten other proteins were identified. Two were cytoplasmic chaperones, four were cytoskeletal proteins, and 4 were proteins that normally reside in the endoplasmic reticulum (ER). The presence of ER proteins in inclusions containing the primarily cytosolic hSOD1 further supports the notion that ER stress is involved in ALS.
|
|
| 3. |
- Björsell, Niclas, et al.
(författare)
-
High dynamic range test-bed for characterization of analog-to-digital converters up to 500 MSPS
- 2005
-
Ingår i: 14th Symposium on New Technologies in Measurement and Instrumentation and 10th Workshop on ADC Modelling and Testing. - : IMEKO-International Measurement Federation Secretariat. - 9788389786371 ; , s. 601-604, s. 601-604
-
Konferensbidrag (refereegranskat)abstract
- A measurement set-up of for the characterization of analog-to-digital converters (ADCs) is described. The measurement set-up characterizes ADCs up to 16 bits at 350 MHz (option for >500 MHz). Testing dynamic performance of high-speed ADCs is regarded as difficult and expensive. By using existing state-of-the-art instruments in combination with specially designed amplifiers and filters, a high performance, cost efficient test-bed has been built-up. Practical performance corresponds to ADC datasheet and exceeds the performance obtained if using commercial instruments only. Consequently, the measurement results represent the true performance of the ADC without impact from the test-bed
|
|
| 4. |
- Blythe, Mark, et al.
(författare)
-
Anti-Solutionist Strategies: Seriously Silly Design Fiction
- 2016
-
Ingår i: Proceedings of the 2016 CHI Conference on Human Factors in Computing Systems (CHI '16). - New York, USA : ACM Press.
-
Konferensbidrag (refereegranskat)abstract
- Much of the academic and commercial work that seeks toinnovate around technology has been dismissed as“solutionist” because it solves problems that don’t exist orignores the complexity of personal, political andenvironmental issues. This paper traces the “solutionism”critique to its origins in city planning and highlights theoriginal concern with imaging and representation in thedesign process. It is increasingly cheap and easy to createcompelling and seductive images of concept designs, whichsell solutions and presume problems. We consider a rangeof strategies, which explicitly reject the search for“solutions”. These include design fiction and critical designbut also less well-known techniques, which aim forunuseless, questionable and silly designs. We present twoexamples of “magic machine” workshops whereparticipants are encouraged to reject realistic premises forpossible technological interventions and create absurdpropositions from lo-fi materials. We argue that suchpractices may help researchers resist the impulse towardssolutionism and suggest that attention to representationduring the ideation process is a key strategy for this.
|
|
| 5. |
- Danielsson, Frida, et al.
(författare)
-
Spatial Characterization of the Human Centrosome Proteome Opens Up New Horizons for a Small but Versatile Organelle
- 2020
-
Ingår i: Proteomics. - : Wiley-VCH Verlag. - 1615-9853 .- 1615-9861.
-
Tidskriftsartikel (refereegranskat)abstract
- After a century of research, the human centrosome continues to fascinate. Based on immunofluorescence and confocal microscopy, an extensive inventory of the protein components of the human centrosome, and the centriolar satellites, with the important contribution of over 300 novel proteins localizing to these compartments is presented. A network of candidate centrosome proteins involved in ubiquitination, including six interaction partners of the Kelch-like protein 21, and an additional network of protein phosphatases, together supporting the suggested role of the centrosome as an interactive hub for cell signaling, is identified. Analysis of multi-localization across cellular organelles analyzed within the Human Protein Atlas (HPA) project shows how multi-localizing proteins are particularly overrepresented in centriolar satellites, supporting the dynamic nature and wide range of functions for this compartment. In summary, the spatial dissection of the human centrosome and centriolar satellites described here provides a comprehensive knowledgebase for further exploration of their proteomes.
|
|
| 6. |
- Ghovanloo, Mohammad-Reza, et al.
(författare)
-
Cannabidiol inhibits the skeletal muscle Nav1.4 by blocking its pore and by altering membrane elasticity
- 2021
-
Ingår i: The Journal of General Physiology. - : Rockefeller University Press. - 0022-1295 .- 1540-7748. ; 153:5
-
Tidskriftsartikel (refereegranskat)abstract
- Cannabidiol (CBD) is the primary nonpsychotropic phytocannabinoid found in Cannabis sativa, which has been proposed to be therapeutic against many conditions, including muscle spasms. Among its putative targets are voltage-gated sodium channels (Navs), which have been implicated in many conditions. We investigated the effects of CBD on Nav1.4, the skeletal muscle Nav subtype. We explored direct effects, involving physical block of the Nav pore, as well as indirect effects, involving modulation of membrane elasticity that contributes to Nav inhibition. MD simulations revealed CBD's localization inside the membrane and effects on bilayer properties. Nuclear magnetic resonance (NMR) confirmed these results, showing CBD localizing below membrane headgroups. To determine the functional implications of these findings, we used a gramicidinbased fluorescence assay to show that CBD alters membrane elasticity or thickness, which could alter Nav function through bilayer-mediated regulation. Site-directed mutagenesis in the vicinity of the Nav1.4 pore revealed that removing the local anesthetic binding site with F1586A reduces the block of INa by CBD. Altering the fenestrations in the bilayer-spanning domain with Nav1.4-WWWW blocked CBD access from the membrane into the Nav1.4 pore (as judged by MD). The stabilization of inactivation, however, persisted in WWWW, which we ascribe to CBD-induced changes in membrane elasticity. To investigate the potential therapeutic value of CBD against Nav1.4 channelopathies, we used a pathogenic Nav1.4 variant, P1158S, which causes myotonia and periodic paralysis. CBD reduces excitability in both wild-type and the P1158S variant. Our in vitro and in silico results suggest that CBD may have therapeutic value against Nav1.4 hyperexcitability.
|
|
| 7. |
- Gudmundsdottir, Valborg, et al.
(författare)
-
Whole blood co-expression modules associate with metabolic traits and type 2 diabetes : an IMI-DIRECT study
- 2020
-
Ingår i: Genome Medicine. - : BioMed Central. - 1756-994X. ; 12:1
-
Tidskriftsartikel (refereegranskat)abstract
- Background: The rising prevalence of type 2 diabetes (T2D) poses a major global challenge. It remains unresolved to what extent transcriptomic signatures of metabolic dysregulation and T2D can be observed in easily accessible tissues such as blood. Additionally, large-scale human studies are required to further our understanding of the putative inflammatory component of insulin resistance and T2D. Here we used transcriptomics data from individuals with (n = 789) and without (n = 2127) T2D from the IMI-DIRECT cohorts to describe the co-expression structure of whole blood that mainly reflects processes and cell types of the immune system, and how it relates to metabolically relevant clinical traits and T2D.Methods: Clusters of co-expressed genes were identified in the non-diabetic IMI-DIRECT cohort and evaluated with regard to stability, as well as preservation and rewiring in the cohort of individuals with T2D. We performed functional and immune cell signature enrichment analyses, and a genome-wide association study to describe the genetic regulation of the modules. Phenotypic and trans-omics associations of the transcriptomic modules were investigated across both IMI-DIRECT cohorts.Results: We identified 55 whole blood co-expression modules, some of which clustered in larger super-modules. We identified a large number of associations between these transcriptomic modules and measures of insulin action and glucose tolerance. Some of the metabolically linked modules reflect neutrophil-lymphocyte ratio in blood while others are independent of white blood cell estimates, including a module of genes encoding neutrophil granule proteins with antibacterial properties for which the strongest associations with clinical traits and T2D status were observed. Through the integration of genetic and multi-omics data, we provide a holistic view of the regulation and molecular context of whole blood transcriptomic modules. We furthermore identified an overlap between genetic signals for T2D and co-expression modules involved in type II interferon signaling.Conclusions: Our results offer a large-scale map of whole blood transcriptomic modules in the context of metabolic disease and point to novel biological candidates for future studies related to T2D.
|
|
| 8. |
- Kathirgamanathar, S, et al.
(författare)
-
Chemistry and bioactivity of physciaceae lichens Pyxine consocians and Heterodermia leucomelos
- 2006
-
Ingår i: Pharmaceutical Biology. - : Informa UK Limited. - 1388-0209 .- 1744-5116. ; 44:3, s. 217-220
-
Tidskriftsartikel (refereegranskat)abstract
- Cabraleadiol monoacetate, atranorin, methyl-beta-orcinolcarboxylate, zeorin, 4- O-methylcryptochlorophaeic acid, and lichexanthone were isolated from the lichen Pyxine consocians Vainio . Heterodermia leucomelos (L). Poelt contained a new triterpenoid, 6 alpha-hydroxyhop-21 beta H-22(29)-en, along with atranorin, zeorin, glyceryl trilinolate, and 3,6-dimethyl-2-hydroxy-4-methoxybenzoic acid. Cabraleadiol monoacetate, 4-O-methylcryptochlorophaeic acid, lichexanthone, and 3,6-dimethyl-2-hydroxy-4-methoxybenzoic acid showed mosquito larvicidal activity against the second instar larvae of Aedes aegypti . Lichexanthone exhibited human sperm motility enhancing activity.
|
|
| 9. |
- Landin, Per Niklas, et al.
(författare)
-
Wideband Characterization of Power Amplifiers Using Undersampling
- 2009
-
Ingår i: 2009 IEEE MTT-S International Microwave Symposium Digest. - Boston : IEEE MTT. - 9781424428038 ; , s. 1365-1368
-
Konferensbidrag (refereegranskat)abstract
- In this paper a radio frequency power amplifier is measured and characterized by the use of undersampling based on the generalized Zhu-Frank sampling theorem. A test system has been designed allowing the bandwidth of the stimuli signal to be 100 MHz in the characterization process. That would not be possible with any vector signal analyzer on the market. One of the more challenging problem within the proposed concept is the model validation process. Here, two different techniques for model validation are proposed, the multitone and the spectrum scan validation methods.
|
|
| 10. |
|
|
