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- Rafati, Nima, et al.
(författare)
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Large Deletions at the SHOX Locus in the Pseudoautosomal Region Are Associated with Skeletal Atavism in Shetland Ponies
- 2016
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Ingår i: G3. - : Oxford University Press (OUP). - 2160-1836. ; 6:7, s. 2213-2223
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Tidskriftsartikel (refereegranskat)abstract
- Skeletal atavism in Shetland ponies is a heritable disorder characterized by abnormal growth of the ulna and fibula that extend the carpal and tarsal joints, respectively. This causes abnormal skeletal structure and impaired movements, and affected foals are usually killed. In order to identify the causal mutation we subjected six confirmed Swedish cases and a DNA pool consisting of 21 control individuals to whole genome resequencing. We screened for polymorphisms where the cases and the control pool were fixed for opposite alleles and observed this signature for only 25 SNPs, most of which were scattered on genome assembly unassigned scaffolds. Read depth analysis at these loci revealed homozygosity or compound heterozygosity for two partially overlapping large deletions in the pseudoautosomal region (PAR) of chromosome X/Y in cases but not in the control pool. One of these deletions removes the entire coding region of the SHOX gene and both deletions remove parts of the CRLF2 gene located downstream of SHOX. The horse reference assembly of the PAR is highly fragmented, and in order to characterize this region we sequenced bacterial artificial chromosome (BAC) clones by single-molecule real-time (SMRT) sequencing technology. This considerably improved the assembly and enabled size estimations of the two deletions to 1602180 kb and 60280 kb, respectively. Complete association between the presence of these deletions and disease status was verified in eight other affected horses. The result of the present study is consistent with previous studies in humans showing crucial importance of SHOX for normal skeletal development.
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| 4. |
- Bertholdsson, Nils-Ove, et al.
(författare)
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Allelopathic potential of Triticum spp., Secale spp. and Triticosecale spp. and use of chromosome substitutions and translocations to improve weed suppression ability in winter wheat
- 2012
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Ingår i: Plant Breeding. - : Wiley. - 0179-9541 .- 1439-0523. ; 131, s. 75-80
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Tidskriftsartikel (refereegranskat)abstract
- A search was carried out for allelopathic sources among accessions of Triticum, Secale, Triticosecale and wheat-rye substitution and translocation lines to be used in breeding programmes to improve weed suppression ability of wheat. A bioassay with mustard as target plants was used for the screening. Mustard was chosen among seven tested target plants because it showed a particular high root growth inhibition when grown together with rye compared with wheat. None of the Triticum accessions studied showed potential allelopathic activity of interest for breeding, but most Triticosecale did. Several of the wheat-rye substitution and translocation lines also showed high allelopathic activity. The highest activity was found in lines with a substitution of 1R or 2R. Some multiple substitution lines and lines with only rye chromatin also showed high allelopathic activity. It is suggested that in vitro selection of wheat-rye substitution lines with high allelopathic potential with a bioassay with mustard as target plants could be used to improve weed suppression ability of wheat.
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- Bubolz, Jéssica, et al.
(författare)
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Genetically modified (GM) late blight-resistant potato and consumer attitudes before and after a field visit
- 2022
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Ingår i: GM Crops and Food Biotechnology in Agriculture and the Food Chain. - : Informa UK Limited. - 2164-5698 .- 2164-5701. ; 13, s. 290-298
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Tidskriftsartikel (refereegranskat)abstract
- Late blight, caused by Phytophthora infestans, is the most devastating disease in potato production. Here, we show full late blight resistance in a location with a genetically diverse pathogen population with the use of GM potato stacked with three resistance (R) genes over three seasons. In addition, using this field trials, we demonstrate that in-the-field intervention among consumers led to change for more favorable attitude generally toward GM crops.
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| 6. |
- Eriksson, Lars-Ove, et al.
(författare)
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Myter och sanningar om svensk fiskodling
- 2003
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Ingår i: Torskar torsken? Forskare och fiskare om fisk och fiske. - Stockholm : Formas. ; , s. 103-111
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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