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Träfflista för sökning "WFRF:(Asp Julia 1973 ) ;pers:(Betsholtz Christer 1959)"

Sökning: WFRF:(Asp Julia 1973 ) > Betsholtz Christer 1959

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1.
  • Asp, Julia, 1973, et al. (författare)
  • Nonradioactive in situ hybridization on frozen sections and whole mounts.
  • 2006
  • Ingår i: Methods in molecular biology (Clifton, N.J.). - 1064-3745. ; 326, s. 89-102
  • Tidskriftsartikel (refereegranskat)abstract
    • Nonradioactive in situ hybridization offers a unique opportunity to study gene expression on samples with preserved histological information. This method makes it possible to locate not only where in a tissue a particular gene is expressed, but in many cases also in which specific cell type it is active. Here, we describe our current protocols for in situ hybridization on frozen sections or whole mounts of mouse embryos. The protocols included describe synthesis of a digoxigenin-labeled probe, tissue handling, hybridization of the probe to the mRNA expressed in the sample and signal detection.
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2.
  • Takemoto, Minoru, et al. (författare)
  • Large-scale identification of genes implicated in kidney glomerulus development and function.
  • 2006
  • Ingår i: The EMBO journal. - : Wiley. - 0261-4189 .- 1460-2075. ; 25:5, s. 1160-74
  • Tidskriftsartikel (refereegranskat)abstract
    • To advance our understanding of development, function and diseases in the kidney glomerulus, we have established and large-scale sequenced cDNA libraries from mouse glomeruli at different stages of development, resulting in a catalogue of 6053 different genes. The glomerular cDNA clones were arrayed and hybridized against a series of labeled targets from isolated glomeruli, non-glomerular kidney tissue, FACS-sorted podocytes and brain capillaries, which identified over 300 glomerular cell-enriched transcripts, some of which were further sublocalized to podocytes, mesangial cells and juxtaglomerular cells by in situ hybridization. For the earliest podocyte marker identified, Foxc2, knockout mice were used to analyze the role of this protein during glomerular development. We show that Foxc2 controls the expression of a distinct set of podocyte genes involved in podocyte differentiation and glomerular basement membrane maturation. The primary podocyte defects also cause abnormal differentiation and organization of the glomerular vascular cells. We surmise that studies on the other novel glomerulus-enriched transcripts identified in this study will provide new insight into glomerular development and pathomechanisms of disease.
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