| 1. |
- Beech, Jason P., et al.
(författare)
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Morphology-based sorting-blood cells and parasites
- 2010
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Ingår i: 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. - 9781618390622 ; 2, s. 1343-1345
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Konferensbidrag (refereegranskat)abstract
- Morphology represents a hitherto unexploited source of specificity in microfluidic particle separation and may serve as the basis for label-free particle fractionation. There is a wealth of morphological changes in blood cells due to a wide range of clinical conditions, diseases, medication and other factors. Also, blood-borne parasites differ in morphology from blood cells. We present the use of Deterministic Lateral Displacement to create a chip-based, label-free diagnostic tool, capable of harvesting some of the wealth of information locked away in red blood cell morphology. We also use the device to separate the parasites that cause sleeping sickness from blood.
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| 2. |
- Holm, Stefan H., et al.
(författare)
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A high-throughput deterministic lateral displacement device for rapid and sensitive field-diagnosis of sleeping sickness
- 2012
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Ingår i: Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. - 9780979806452 ; , s. 530-532
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Konferensbidrag (refereegranskat)abstract
- We present a simple and rapid microfluidic device capable of extracting and concentrating the parasite causing the fatal disease sleeping sickness (SS) from blood. The device is based on deterministic lateral displacement (DLD) and constructed with a single inlet with flow induced by an ordinary syringe. The simplicity is crucial as the device is intended for use in the resource depraved areas where the disease is endemic. With only one inlet an intricate design with multiple depths has been utilized to create a cell free stream from the blood plasma into which the parasites are forced and subsequently collected in a detection region. In order to maximize the sample volume up to 10 device layers were stacked on top of each other which resulted in a throughput of ∼10 μL/min. This allowed for an approximate time per test of below 15 min.
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| 3. |
- Holm, Stefan H., et al.
(författare)
-
Multiple depths in a deterministic lateral displacement device for field-diagnosis of sleeping-sickness
- 2011
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Ingår i: 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. - 9781618395955 ; 1, s. 527-529
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Konferensbidrag (refereegranskat)abstract
- We present a simple and inexpensive device capable of extracting and concentrating the parasite causing sleeping sickness from blood. The device is aimed at being used in rural resource depraved areas where the disease is endemic; therefore simplicity is of paramount importance. The device is based on deterministic lateral displacement with a single inlet and flow induced by a syringe. Through an intricate design with multiple depths, a cell free stream is created from the blood plasma into which the parasites are guided and subsequently collected in a dedicated reservoir for observation.
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| 4. |
- Regnault, C., et al.
(författare)
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Microfluidic separation of parasites and parasite-infected cells from blood for the diagnosis of leishmaniasis
- 2016
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Ingår i: 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. - 9780979806490 ; , s. 244-245
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Konferensbidrag (refereegranskat)abstract
- Microfluidic techniques were applied to the separation of parasite and parasite-infected cells from blood to facilitate the detection of leishmaniasis, a disease representing a high burden in the developing world and for which new diagnostic tools are urgently needed. Leishmania mexicana promastigotes were successfully separated from red blood cells in a deterministic lateral displacement device. The mechanical properties of macrophages infected with L. mexicana were investigated using real-time deformability cytometry. In the early stage, we find that macrophages deform less than the control. The trend is reversed four days post infection while we see a continuous increase in cell size after parasitization.
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