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Animal-Free Human Whole Blood Sepsis Model to Study Changes in Innate Immunity
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- Messerer, David Alexander Christian (författare)
- Univ Hosp Ulm, Inst Clin & Expt Trauma Immunol, Ulm, Germany.;Univ Hosp Ulm, Dept Anesthesiol & Intens Care Med, Ulm, Germany.
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- Vidoni, Laura (författare)
- Univ Hosp Ulm, Inst Clin & Expt Trauma Immunol, Ulm, Germany.
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- Erber, Maike (författare)
- Univ Hosp Ulm, Inst Clin & Expt Trauma Immunol, Ulm, Germany.
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- Stratmann, Alexander Elias Paul (författare)
- Univ Hosp Ulm, Inst Clin & Expt Trauma Immunol, Ulm, Germany.
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- Bauer, Jonas Martin (författare)
- Univ Hosp Ulm, Inst Clin & Expt Trauma Immunol, Ulm, Germany.
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- Braun, Christian Karl (författare)
- Univ Hosp Ulm, Inst Clin & Expt Trauma Immunol, Ulm, Germany.
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- Hug, Stefan (författare)
- Univ Hosp Ulm, Inst Clin & Expt Trauma Immunol, Ulm, Germany.
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- Adler, Anna (författare)
- Uppsala universitet,Klinisk immunologi
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- Nilsson Ekdahl, Kristina (författare)
- Uppsala universitet,Linnéuniversitetet,Institutionen för kemi och biomedicin (KOB),Avancerade material,Uppsala university, Sweden,Linnaeus Ctr Biomat Chem, BMC,Klinisk immunologi,Linnaeus Univ, Ctr Biomat Chem, Kalmar, Sweden.
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- Nilsson, Bo (författare)
- Uppsala universitet,Klinisk immunologi
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- Barth, Eberhard (författare)
- Univ Hosp Ulm, Dept Anesthesiol & Intens Care Med, Ulm, Germany.
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- Radermacher, Peter (författare)
- Ulm Univ, Inst Anesthesiol Pathophysiol & Proc Engn, Ulm, Germany.
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- Huber-Lang, Markus (författare)
- Univ Hosp Ulm, Inst Clin & Expt Trauma Immunol, Ulm, Germany.
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Univ Hosp Ulm, Inst Clin & Expt Trauma Immunol, Ulm, Germany;Univ Hosp Ulm, Dept Anesthesiol & Intens Care Med, Ulm, Germany. Univ Hosp Ulm, Inst Clin & Expt Trauma Immunol, Ulm, Germany. (creator_code:org_t)
- 2020-10-14
- 2020
- Engelska.
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Ingår i: Frontiers in Immunology. - : Frontiers Media S.A.. - 1664-3224. ; 11, s. 1-12
- Relaterad länk:
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https://doi.org/10.3...
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https://www.frontier...
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https://uu.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.3...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Studying innate immunity in humans is crucial for understanding its role in the pathophysiology of systemic inflammation, particularly in the complex setting of sepsis. Therefore, we standardized a step-by-step process from the venipuncture to the transfer in a human model system, while closely monitoring the inflammatory response for up to three hours. We designed an animal-free, human whole blood sepsis model using a commercially available, simple to use, tubing system. First, we analyzed routine clinical parameters, including cell count and blood gas analysis. Second, we demonstrated that extracellular activation markers (e.g., CD11b and CD62l) as well as intracellular metabolic (intracellular pH) and functional (generation of radical oxygen species) features remained stable after incubation in the whole blood model. Third, we mimicked systemic inflammation during early sepsis by exposure of whole blood to pathogen-associated molecular patterns. Stimulation with lipopolysaccharide revealed the capability of the model system to evoke a sepsis-like inflammatory phenotype of innate immunity. In summary, the presented model serves as a convenient, economic, and reliable platform to study innate immunity in human whole blood, which may yield clinically important insights.
Ämnesord
- NATURVETENSKAP -- Biologi -- Immunologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Immunology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Nyckelord
- inflammation
- neutrophil granulocytes
- lipopolysaccharide (LPS)
- blood physiology
- sepsis
- ex vivo whole blood model
- principles of the 3Rs
- Immunologi
- Immunology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Messerer, David ...
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Vidoni, Laura
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Erber, Maike
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Stratmann, Alexa ...
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Bauer, Jonas Mar ...
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Braun, Christian ...
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Hug, Stefan
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Adler, Anna
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Nilsson Ekdahl, ...
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Nilsson, Bo
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Barth, Eberhard
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Radermacher, Pet ...
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Huber-Lang, Mark ...
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