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Träfflista för sökning "WFRF:(Beech Jason P.) "

Sökning: WFRF:(Beech Jason P.)

  • Resultat 1-10 av 57
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  • Ohlsson, Gabriel, 1982, et al. (författare)
  • Solute transport on the sub 100 ms scale across the lipid bilayer membrane of individual proteoliposomes
  • 2012
  • Ingår i: Lab on a Chip. - : Royal Society of Chemistry. - 1473-0197 .- 1473-0189. ; 12:22, s. 4635-4643
  • Tidskriftsartikel (refereegranskat)abstract
    • Screening assays designed to probe ligand and drug-candidate regulation of membrane proteins responsible for ion-translocation across the cell membrane are wide spread, while efficient means to screen membrane-protein facilitated transport of uncharged solutes are sparse. We report on a microfluidic-based system to monitor transport of uncharged solutes across the membrane of multiple (>100) individually resolved surface-immobilized liposomes. This was accomplished by rapidly switching (<10 ms) the solution above dye-containing liposomes immobilized on the floor of a microfluidic channel. With liposomes encapsulating the pH-sensitive dye carboxyfluorescein (CF), internal changes in pH induced by transport of a weak acid (acetic acid) could be measured at time scales down to 25 ms. The applicability of the set up to study biological transport reactions was demonstrated by examining the osmotic water permeability of human aquaporin (AQP5) reconstituted in proteoliposomes. In this case, the rate of osmotic-induced volume changes of individual proteoliposomes was time resolved by imaging the self quenching of encapsulated calcein in response to an osmotic gradient. Single-liposome analysis of both pure and AQP5-containing liposomes revealed a relatively large heterogeneity in osmotic permeability. Still, in the case of AQP5-containing liposomes, the single liposome data suggest that the membrane-protein incorporation efficiency depends on liposome size, with higher incorporation efficiency for larger liposomes. The benefit of low sample consumption and automated liquid handling is discussed in terms of pharmaceutical screening applications.
  • Beech, J P, et al. (författare)
  • Stretching the limits of separation
  • 2006
  • Ingår i: Book of abstracts: Intl Conf on Nanosci and Technol, Basel, Switzerland (2006).
  • Konferensbidrag (refereegranskat)
  • Beech, Jason, et al. (författare)
  • Sorting cells by size, shape and deformability.
  • 2012
  • Ingår i: Lab on a Chip. - : Royal Society of Chemistry. - 1473-0189 .- 1473-0197. ; 12:6, s. 1048-1051
  • Tidskriftsartikel (refereegranskat)abstract
    • While size has been widely used as a parameter in cellular separations, in this communication we show how shape and deformability, a mainly untapped source of specificity in preparative and analytical microfluidic devices can be measured and used to separate cells.
  • Bogas, Diana, et al. (författare)
  • Applications of optical DNA mapping in microbiology
  • 2017
  • Ingår i: BioTechniques. - : Informa Healthcare. - 0736-6205. ; 62:6, s. 255-267
  • Forskningsöversikt (refereegranskat)abstract
    • Optical mapping (OM) has been used in microbiology for the past 20 years, initially as a technique to facilitate DNA sequence-based studies; however, with decreases in DNA sequencing costs and increases in sequence output from automated sequencing platforms, OM has grown into an important auxiliary tool for genome assembly and comparison. Currently, there are a number of new and exciting applications for OM in the field of microbiology, including investigation of disease outbreaks, identification of specific genes of clinical and/or epidemiological relevance, and the possibility of single-cell analysis when combined with cell-sorting approaches. In addition, designing lab-on-a-chip systems based on OM is now feasible and will allow the integrated and automated microbiological analysis of biological fluids. Here, we review the basic technology of OM, detail the current state of the art of the field, and look ahead to possible future developments in OM technology for microbiological applications.
  • Persson, Henrik, et al. (författare)
  • Vertical oxide nanotubes connected by subsurface microchannels
  • 2012
  • Ingår i: Nano Reseach. - : Springer. - 1998-0124 .- 1998-0000. ; 5:3, s. 190-198
  • Tidskriftsartikel (refereegranskat)abstract
    • We describe the fabrication of arrays of oxide nanotubes using a combination of bottom up and top down nanofabrication. The nanotubes are made from epitaxially grown semiconductor nanowires that are covered with an oxide layer using atomic layer deposition. The tips of the oxide-covered nanowires are removed by argon sputtering and the exposed semiconductor core is then selectively etched, leaving a hollow oxide tube. We show that it is possible to create fluidic connections to the nanotubes by a combination of electron beam lithography to precisely define the nanotube positions and controlled wet under-etching. DNA transport is demonstrated in the microchannel. Cells were successfully cultured on the nanotube arrays, demonstrating compatibility with cell-biological applications. Our device opens up the possibility of injecting molecules into cells with both spatial and temporal control.
  • Westerlund, Fredrik, et al. (författare)
  • Fluorescence microscopy of nanochannel-confined DNA
  • 2018
  • Ingår i: Methods in Molecular Biology. - : Humana Press. - 1064-3745. ; 1665, s. 173-198
  • Bokkapitel (refereegranskat)abstract
    • Stretching of DNA in nanoscale confinement allows for several important studies. The genetic contents of the DNA can be visualized on the single DNA molecule level and both the polymer physics of confined DNA and also DNA/protein and other DNA/DNA-binding molecule interactions can be explored. This chapter describes the basic steps to fabricate the nanostructures, perform the experiments and analyze the data.
  • Barrett, Michael P., et al. (författare)
  • Microfluidics-based approaches to the isolation of African trypanosomes
  • 2017
  • Ingår i: Pathogens. - : MDPI AG. - 2076-0817. ; 6:4
  • Forskningsöversikt (refereegranskat)abstract
    • African trypanosomes are responsible for significant levels of disease in both humans and animals. The protozoan parasites are free-living flagellates, usually transmitted by arthropod vectors, including the tsetse fly. In the mammalian host they live in the bloodstream and, in the case of human-infectious species, later invade the central nervous system. Diagnosis of the disease requires the positive identification of parasites in the bloodstream. This can be particularly challenging where parasite numbers are low, as is often the case in peripheral blood. Enriching parasites from body fluids is an important part of the diagnostic pathway. As more is learned about the physicochemical properties of trypanosomes, this information can be exploited through use of different microfluidic-based approaches to isolate the parasites from blood or other fluids. Here, we discuss recent advances in the use of microfluidics to separate trypanosomes from blood and to isolate single trypanosomes for analyses including drug screening.
  • Beech, Jason P., et al. (författare)
  • Morphology-based sorting-blood cells and parasites
  • 2010
  • Ingår i: 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. - 9781618390622 ; 2, s. 1343-1345
  • Konferensbidrag (refereegranskat)abstract
    • Morphology represents a hitherto unexploited source of specificity in microfluidic particle separation and may serve as the basis for label-free particle fractionation. There is a wealth of morphological changes in blood cells due to a wide range of clinical conditions, diseases, medication and other factors. Also, blood-borne parasites differ in morphology from blood cells. We present the use of Deterministic Lateral Displacement to create a chip-based, label-free diagnostic tool, capable of harvesting some of the wealth of information locked away in red blood cell morphology. We also use the device to separate the parasites that cause sleeping sickness from blood.
  • Beech, Jason P., et al. (författare)
  • Separation of pathogenic bacteria by chain length
  • 2018
  • Ingår i: Analytica Chimica Acta. - : Elsevier. - 0003-2670 .- 1873-4324. ; 1000, s. 223-231
  • Tidskriftsartikel (refereegranskat)abstract
    • Using Deterministic Lateral Displacement devices optimized for sensitivity to particle length, we separate subpopulations of bacteria depending on known properties that affect their capability to cause disease (virulence). For the human bacterial pathogen Streptococcus pneumoniae, bacterial chain length and the presence of a capsule are known virulence factors contributing to its ability to cause severe disease. Separation of cultured pneumococci into subpopulations based on morphological type (single cocci, diplococci and chains) will enable more detailed studies of the role they play in virulence. Moreover, we present separation of mixed populations of almost genetically identical encapsulated and non-encapsulated pneumococcal strains in our device.
  • Holm, Stefan H., et al. (författare)
  • A high-throughput deterministic lateral displacement device for rapid and sensitive field-diagnosis of sleeping sickness
  • 2012
  • Ingår i: Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. - : Chemical and Biological Microsystems Society. - 9780979806452 ; , s. 530-532
  • Konferensbidrag (refereegranskat)abstract
    • We present a simple and rapid microfluidic device capable of extracting and concentrating the parasite causing the fatal disease sleeping sickness (SS) from blood. The device is based on deterministic lateral displacement (DLD) and constructed with a single inlet with flow induced by an ordinary syringe. The simplicity is crucial as the device is intended for use in the resource depraved areas where the disease is endemic. With only one inlet an intricate design with multiple depths has been utilized to create a cell free stream from the blood plasma into which the parasites are forced and subsequently collected in a detection region. In order to maximize the sample volume up to 10 device layers were stacked on top of each other which resulted in a throughput of ∼10 μL/min. This allowed for an approximate time per test of below 15 min.
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  • [1]23456Nästa
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