| 1. |
- Lard, Mercy, et al.
(författare)
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Use of dielectrophoresis for directing T cells to microwells before nanostraw transfection : modelling and experiments
- 2022
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Ingår i: RSC Advances. - : Royal Society of Chemistry (RSC). - 2046-2069. ; 12:47, s. 30295-30303
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Tidskriftsartikel (refereegranskat)abstract
- Nanostraw substrates have great potential for achieving minimally invasive cell transfection. Cells located on the nanostraw substrate are subjected to mild DC electric pulses applied across the nanostraw substrate, which open pores in the cell membrane on top of the nanostraws and drives charged cargo through these pores via electrophoresis. However, with this method, the current may leak through uncovered nanostraws, thereby decreasing the desired effect in the cell-covered nanostraws. A minimization of the number of uncovered nanostraws could be achieved by high cell coverage, but this is challenging when working with small cell populations. Nanostraw substrates of smaller area could be covered by smaller cell populations but are hard to integrate into fluidics systems. Here, we use simulations and experiments to show that this issue can be addressed by covering the nanostraw substrate with an insulating layer containing pores of similar size to cells. The pores act as traps into which cells can be guided using dielectrophoresis, ensuring a high degree of occupancy while maintaining a high cell viability, even if the total number of cells is low.
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| 2. |
- Yadegari, Farnaz, et al.
(författare)
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Microfluidic connections to hollow nanowires
- 2011
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Ingår i: 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. - 9781618395955 ; 1, s. 386-388
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Konferensbidrag (refereegranskat)abstract
- We have fabricated a device in which hollow nanowires (HNWs) lying on a glass surface are connected to microfluidic channels for the purpose of guiding DNA and to characterize the fluidic properties of our HNWs. The dimensions of our HNWs (inner diameter 10-80 nm, outer diameter 50-200 nm and length several μm) can be made on a scale much smaller than the size of a cell and on the same order of magnitude as the Debye length, making them interesting both for applications involving direct chemical access to the cytosol of living cells and for investigations of basic transport phenomena.
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