| 1. |
- Berglund, Johan C, 1978, et al.
(författare)
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Measuring strategies for smooth tool steel surfaces
- 2008
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Ingår i: Proceedings. - Aachen : Shaker Verlag. - 1610-4773. - 9783832269128 - 3832269126 ; , s. 110-119
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- Comparisons between different measuring strategies were made on three types of smooth tool steel surfaces. Three replica materials were tested to study possibilities within replication techniques. An optical interferometer as well as a mechanical stylus was used to evaluate the surfaces. The results showed that the tested replica materials generated good representations of both the form and the surface roughness (Sq > 300 nm). The evaluated surfaces were quite homogeneous, thus, few measurements are needed to get representative results. However, it was found that caution must be taken regarding manually polished surfaces which can be less homogenous and therefore require more measurements to get representative results.
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| 2. |
- Milenkovski, Susann, et al.
(författare)
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Composition of denitrifying bacterial enzyme genes nirS, nirK and nosZ in constructed wetlands
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- In this study the composition of the denitrifying bacterial community among constructed wetlands in agricultural areas was investigated. Thirty-two constructed wetlands located in Southern Sweden were surveyed, and biofilm samples from each were analyzed by applying denaturing gradient gel electrophoresis, to investigate the community composition of the three denitrifying bacterial enzyme genes nirK, nirS and nosZ. The DNA sequences of the enzyme genes were compared to known DNA sequences in GeneBank using BLAST. The results of the denitrifying bacterial enzyme genes indicated that these habitats may harbour a heterogeneous denitrifying bacterial community. Individual analysis of the enzyme genes revealed that nirS was more heterogeneous than both nirK and nosZ. Most sequences from the present study clustered with known sequences from species belonging to the group of α-Proteobacteria, and to a lesser extent with β- Proteobacteria and γ-Proteobacteria, and only nirS clustered with a member of gram-positive bacteria.
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| 3. |
- Milenkovski, Susann, et al.
(författare)
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Variation of eubacterial and denitrifying bacterial biofilm communities among constructed wetlands
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- Bacteria play important roles in the transformation of nutrients in wetlands, but few studies have examined parameters affecting variation in bacterial community composition between wetlands. We compared the composition of eubacterial and denitrifying bacterial biofilm communities in 32 agricultural constructed wetlands in southern Sweden, and the extent to which wetland environmental parameters could explain the observed variation. Structure and richness of the eubacterial 16S rRNA gene and three denitrifying bacterial enzyme genes (nirK, nirS and nosZ), analysed by molecular fingerprinting methods, varied among the constructed wetlands, which could be partly explained by different environmental parameters. Results from the enzyme gene analyses were also compared to determine whether the practice of using a single denitrifying bacterial gene could characterize the overall community composition of denitrifying bacteria. We found that nirK was more diverse than both nirS and the nosZ, and the band structure and richness of the three genes were not related to the sam environmental parameters. This suggests that using a single enzyme gene may not suffice to characterize the community composition of denitrifying bacteria in constructed agricultural wetlands.
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