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- Nilsson, R. Henrik, 1976, et al.
(author)
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Improving ITS sequence data for identification of plant pathogenic fungi
- 2014
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In: Fungal Diversity. - : Springer Science and Business Media LLC. - 1560-2745 .- 1878-9129. ; 67:1, s. 11-19
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Journal article (peer-reviewed)abstract
- Plant pathogenic fungi are a large and diverse assemblage of eukaryotes with substantial impacts on natural ecosystems and human endeavours. These taxa often have complex and poorly understood life cycles, lack observable, discriminatory morphological characters, and may not be amenable to in vitro culturing. As a result, species identification is frequently difficult. Molecular (DNA sequence) data have emerged as crucial information for the taxonomic identification of plant pathogenic fungi, with the nuclear ribosomal internal transcribed spacer (ITS) region being the most popular marker. However, international nucleotide sequence databases are accumulating numerous sequences of compromised or low-resolution taxonomic annotations and substandard technical quality, making their use in the molecular identification of plant pathogenic fungi problematic. Here we report on a concerted effort to identify high-quality reference sequences for various plant pathogenic fungi and to re-annotate incorrectly or insufficiently annotated public ITS sequences from these fungal lineages. A third objective was to enrich the sequences with geographical and ecological metadata. The results – a total of 31,954 changes – are incorporated in and made available through the UNITE database for molecular identification of fungi (http://unite.ut.ee), including standalone FASTA files of sequence data for local BLAST searches, use in the next-generation sequencing analysis platforms QIIME and mothur, and related applications. The present initiative is just a beginning to cover the wide spectrum of plant pathogenic fungi, and we invite all researchers with pertinent expertise to join the annotation effort.
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| 2. |
- Berglund, Jonatan, 1983, et al.
(author)
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Production system geometry assurance using 3D imaging
- 2016
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In: Procedia CIRP. - : Elsevier BV. - 2212-8271. ; 44, s. 132-137
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Conference paper (peer-reviewed)abstract
- Production systems evolve to accommodate new and redesigned products. These changes are planned offline in virtual tools, to reduce disturbances on ongoing production. Offline planning requires virtual models that correctly represent reality. Most models are "as-designed" and suffer from geometrical errors stemming from deployment alterations. Such errors are often discovered late in the next change process or during installation, making corrections expensive. Having geometry assured production systems and models eliminate one source of error during the production system change process. This paper evaluates 3D imaging and the C2M (cloud-to-mesh) algorithm for assessing the validity of virtual production system models.
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| 4. |
- Carlström, Eric, 1957, et al.
(author)
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Inter-Organisational Exercises in Dry and Wet Context-Why Do Maritime Response Organisations Gain More Knowledge from Exercises at Sea Than Those on Shore?
- 2020
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In: Sustainability. - : MDPI AG. - 2071-1050. ; 12:14
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Journal article (peer-reviewed)abstract
- This is a study of inter-organisational exercises arranged by on-shore organisations (ONSOs) and off-shore organisations (OFFSOs). The aim was to compare findings from trained emergency staffs' perceptions of the impact of exercises. The data were retrieved from surveys conducted by the research team in conjunction with exercises. The surveys included staff from the coast guard, sea rescue, police department, fire department and ambulance services. A total of 94 professional emergency personnel participated in the ONSO exercises and 252 in the OFFSO exercises. The study was based on the suggestion that collaborative elements during an inter-organisational exercise promote learning, and learning is important to make the exercises useful. Collaboration proved to be a predictor for some of the items in learning, and learning was a predictor for some of the items in utility. There was, however, a stronger covariation between collaboration, learning and utility in the OFFSOs exercises than in the ONSOs. One reason might be the different cultures of emergency staff involved in on-shore and off-shore organisations. The OFFSOs' qualifications may be dominated by seamanship, together with professional practice, and all parties are expected to act as first responders. ONSOs, on the other hand, practice exercises from a strict professional and legal perspective.
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