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- Björklund, Elisabet, et al.
(författare)
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Quality control of flow cytometry data analysis for evaluation of minimal residual disease in bone marrow from acute leukemia patients during treatment.
- 2009
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Ingår i: Journal of pediatric hematology/oncology : official journal of the American Society of Pediatric Hematology/Oncology. - : Lippincott Williams & Wilkins. - 1536-3678 .- 1077-4114. ; 31:6, s. 406-15
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Tidskriftsartikel (refereegranskat)abstract
- Low levels of leukemia cells in the bone marrow, minimal residual disease (MRD), are considered to be a powerful indicator of treatment response in acute lymphatic leukemia (ALL). A Nordic quality assurance program, aimed on standardization of the flow cytometry MRD analysis, has been established before implementation of MRD at cutoff level 10 as one of stratifying parameters in next Nordic Society of Pediatric Hematology and Oncology (NOPHO) treatment program for ALL. In 4 quality control (QC) rounds 15 laboratories determined the MRD levels in 48 follow-up samples from 12 ALL patients treated according to NOPHO 2000. Analysis procedures were standardized. For each QC round a compact disc containing data in list-mode files was sent out and results were submitted to a central laboratory. At cutoff level 10, which will be applied for clinical decisions, laboratories obtained a high concordance (91.6%). If cutoff level 10 was applied, the concordance would be lower (85.3%). The continuing standardization resulted in better concordance in QC3 and QC4 compared with QC1 and QC2. The concordance was higher in precursor B as compared with T-cell ALL. We conclude that after standardization, flow cytometry MRD detection can be reliably applied in international, multicenter treatment protocols.
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| 4. |
- Mageroy, Melissa H., et al.
(författare)
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Priming of inducible defenses protects Norway spruce against tree‐killing bark beetles
- 2020
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Ingår i: Plant, Cell and Environment. - : John Wiley & Sons. - 0140-7791 .- 1365-3040. ; 43:2, s. 420-430
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Tidskriftsartikel (refereegranskat)abstract
- Plants can form an immunological memory known as defense priming, whereby exposure to a priming stimulus enables quicker or stronger response to subsequent attack by pests and pathogens. Such priming of inducible defenses provides increased protection and reduces allocation costs of defense. Defense priming has been widely studied for short–lived model plants such as Arabidopsis, but little is known about this phenomenon in long‐lived plants like spruce. We compared the effects of pre‐treatment with sub‐lethal fungal inoculations or application of the phytohormone methyl jasmonate (MeJA) on the resistance of 48‐year‐old Norway spruce (Picea abies) trees to mass attack by a tree‐killing bark beetle beginning 35 days later. Bark beetles heavily infested and killed untreated trees, but largely avoided fungus‐inoculated trees and MeJA‐treated trees. Quantification of defensive terpenes at the time of bark beetle attack showed fungal inoculation induced 91‐fold higher terpene concentrations compared to untreated trees, while application of MeJA did not significantly increase terpenes. These results indicate that resistance in fungus‐inoculated trees is a result of direct induction of defenses while resistance in MeJA‐treated trees is due to defense priming. This work extends our knowledge of defense priming from model plants to an ecologically important tree species.
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| 5. |
- Sädbom, Stefan, et al.
(författare)
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The use of systematic sampling and XRF-XRT based scanning to determine potential recovery of metals from waste rock
- 2020
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Ingår i: Mine Water Solutions. - : International Mine Water Association. - 9783000672972 ; , s. 146-151
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Konferensbidrag (refereegranskat)abstract
- It is difficult to evaluate the potential for reprocessing and extraction of minerals from waste rock with valuable and/or harmful elements.We suggest a new sampling strategy/protocol for waste rock, specifically developed for historic mining sites, in combination with XRF-XRT scanning with a GeoCore X10 instrument.Håkansboda historical mine site in Sweden was used as a case study to look at the potential for the combination of techniques.The combination of the suggested randomized sampling strategy/protocol and the dataset from the GX10 scanning enables prediction of amenability for pre-processing with the use of mechanical sorting or if the extraction of valuable minerals only can be achieved through fine grinding, flotation or leaching.
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