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- Ehinger, Anna, et al.
(författare)
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Myoepithelium assessment with p63 immunostaining in formalinfixed paraffin-embedded breast cancer tissue pre-treated with RNA-later
- 2017
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Ingår i: Virchows Archiv. - : Springer Science and Business Media LLC. - 1432-2307 .- 0945-6317. ; 471:Supplement 1, s. 299-299
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Konferensbidrag (refereegranskat)abstract
- Objective: To assessmyoepithelium with p63 in fresh breast cancer (BC)tissue samples collected in RNA later for further analysis with NextGeneration Sequencing (NGS) technique. For a better understanding ofthe NGS bulk-analysis, a central part of the sample in RNA-later isformalin-fixed paraffin-embedded to score relative cellularity in % onhematoxylin-eosin (HE) staining (% of invasive cancer, cancer in situ,benign epithelium, lymphocytes and fat). Our aim is hence to test p63immunohistochemistry (IHC) to highlight myoepithelium and to facilitatethe evaluation of the relative cellularity on BC-tissue pre-treated withRNA-later.Method: Two-hundred and twenty-four selected samples of fresh BCtissue collected in RNA-later. A 10 mg central piece from each samplewas FFPE and assembled in a tissue-microarray (TMA) and sectioned toHE and p63 IHC.Results: All samples (n = 224) had internal control for myoepitheliumsurrounding in situ cancer or benign epithelium. p63 showed positivenuclear staining in myoepithelial cells in 92 % (206/224) of samplesand false negative p63 staining in 8 % (18/224).Conclusion: p63 IHC is assessable in samples of FFPE BC-tissue pretreatedwith RNA-later.
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