Sökning: WFRF:(Brantsing Camilla)
> Ohlsson Claes 1965 >
Cortisol decreases ...
Cortisol decreases IGF-I mRNA levels in human osteoblast-like cells.
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- Swolin-Eide, Diana, 1968 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kvinnors och barns hälsa, Avdelningen för pediatrik,Institute for the Health of Women and Children, Dept of Paediatrics
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- Brantsing, Camilla (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för laboratoriemedicin, Avdelningen för klinisk kemi/transfusionsmedicin,Institute of Laboratory Medicine, Dept of Clinical Chemistry/Transfusion Medicine
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- Matejka, Göran (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin,Institute of Internal Medicine
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- Ohlsson, Claes, 1965 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för internmedicin,Institute of Internal Medicine, Dept of Medicine
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visa färre...
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(creator_code:org_t)
- 1996
- 1996
- Engelska.
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Ingår i: The Journal of endocrinology. - 0022-0795. ; 149:3, s. 397-403
- Relaterad länk:
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https://gup.ub.gu.se...
Abstract
Ämnesord
Stäng
- Excess levels of glucocorticoids are known to cause osteoporosis. It is speculated that the effect of glucocorticoids could be mediated via regulation of IGF-I. The aims of the present study were to detect and quantify the expression of IGF-I and/or IGF-II mRNA transcripts in human osteoblast-like cells and to investigate whether glucocorticoids regulate the expression of IGF-I mRNA transcripts in human osteoblast-like cells. Cultures of human osteoblast-like cells from trabecular bone were established. The IGF-IA and IGF-IB transcripts were detected in human osteoblast-like cells from seven out of nine patients while the IGF-II transcript was detected in human osteoblast-like cells from eight out of nine patients, as determined by RT-PCR assays. Human osteoblast-like cells, as well as human muscle tissue, expressed approximately 1/10 of the IGF-I mRNA levels found in liver, as determined by RNase protection solution hybridization assay. The IGF-I mRNA levels did not decrease with age in the human osteoblast-like cells and no difference was seen between males and females. However, cortisol (10(-6) mol/l) decreased IGF-I mRNA levels. In summary, the present study has shown that human osteoblast-like cells express IGF-I and IGF-II mRNA transcripts and that cortisol down-regulates the IGF-I mRNA levels, indicating that some of the inhibitory effect of glucocorticoids on bone formation in humans is mediated via a reduced autocrine/paracrine expression of IGF-I.
Nyckelord
- Adolescent
- Adult
- Base Sequence
- Cells
- Cultured
- Child
- Child
- Preschool
- DNA Primers
- genetics
- Down-Regulation
- Female
- Gene Expression
- drug effects
- Humans
- Hydrocortisone
- pharmacology
- Infant
- Insulin-Like Growth Factor I
- genetics
- metabolism
- Insulin-Like Growth Factor II
- genetics
- Male
- Molecular Sequence Data
- Osteoblasts
- drug effects
- metabolism
- Polymerase Chain Reaction
- RNA
- Messenger
- metabolism
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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