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Sökning: WFRF:(Brismar Hjalmar) > Konferensbidrag

  • Resultat 1-10 av 23
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  • Ardabili, Sahar, et al. (författare)
  • Dean flow-coupled inertial focusing for ultra-high-throughput particle filtration
  • 2010
  • Ingår i: 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. - 9781618390622 ; , s. 1586-1588
  • Konferensbidrag (refereegranskat)abstract
    • Particle manipulation represents an important and fundamental step prior to counting, sorting and detecting bio-particles. In this study, we report dean-coupled inertial focusing of particles in flows through a single curve microchannel at extremely high channel Reynold numbers (∼325). We found the lateral particle focusing position, xf to be fixed and largely independent of radius of curvature and whether particles are pre-focused (at equilibrium) entering the curvature or randomly distributed. Finally, using a single inlet, u-shaped, microchannel we demonstrate filtration of 10μm particles from 2 μm particles at throughputs several orders of magnitude higher than previously shown.
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  • Errando-Herranz, Carlos, et al. (författare)
  • Biocompatibility of OSTE polymers studied by cell growth experiments
  • 2013
  • Ingår i: Proceedings of the 17th Int. Conf. on Miniaturized Systems for Chemistry  and Life Sciences (microTAS). - Freiburg, Germany.
  • Konferensbidrag (refereegranskat)abstract
    • The recently introduced OSTE polymer technology has shown very useful features for microfluidics for lab-on-a-chip applications. However, no data has yet been published on cell viability on OSTE. In this work, we study the biocompatibility of three OSTE formulations by cell growth experiments. Moreover, we investigate the effect of varying thiol excess on cell viability on OSTE surfaces. The results show poor cell viability on one OSTE formulation, and viability comparable with polystyrene on a second formulation with thiol excess below 60%. In the third formulation, we observe cell proliferation. These results are promising for cell-based assays in OSTE microfluidic devices.
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  • Frisk, Thomas, et al. (författare)
  • Cultivation of COS7-cells using extracellular matrix in 3D microfluidic surface enlarged structure
  • 2005
  • Ingår i: Micro Total Analysis Systems 2004. - 0854048960 ; , s. 261-263
  • Konferensbidrag (refereegranskat)abstract
    • This paper presents a novel method to cultivate cells in a controlled 3D surface enlarged micro-environment. The 3D environment is achieved by insertion of a gel-like extracellular matrix (ECM) mixed with cells into a micromachined silicon fluid structure. Shrinking of the gel enables further flow through the channel. Due to the structure design the shrinking is non-uniform, which results in an increased surface area. With the proposed design cells are alive and viable after 72 h of incubation within the chip.
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  • Frisk, T., et al. (författare)
  • Live-cell imaging of natural killer cell mediated tumor rejection in arrays of microwells
  • 2010
  • Ingår i: 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. - 9781618390622 ; , s. 950-952
  • Konferensbidrag (refereegranskat)abstract
    • Due to the inherent heterogeneity of immune cell populations a comprehensive view of immune functions can only be achieved by collecting data from many individual cells. We here demonstrate a novel multi-well microchip platform for cell analysis, which we use to study the interaction between natural killer (NK) cells and their target cells.
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  • Frisk, Thomas, et al. (författare)
  • Three dimensional asymmetric microenvironment for cell biological studies
  • 2005
  • Ingår i: Proceedings of µTAS 2005 Conference. ; , s. 915-917
  • Konferensbidrag (refereegranskat)abstract
    • We report on a method for three-dimensional cultivation of cells in a microstructured asymmetric environment. In the system an asymmetric environment is created by using diffusion through a gel of extra cellular matrix proteins surrounded by microfluidic flow channels. Individual cells embedded in the gel react on the concentration gradient. The system has been evaluated both for diffusion properties and based on the cellular response.
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  • Hultström, Jessica, et al. (författare)
  • Proliferation and viability of COS-7 cells trapped by standing-wave ultrasound in a microfluidic chip
  • 2006
  • Ingår i: Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference. - : Japan Academic Association Inc. ; , s. 449-451
  • Konferensbidrag (refereegranskat)abstract
    • We study cell viability after ultrasonic-standing-wave trapping of low cell numbers in a microfluidic chip by recultivation of the trapped cells. The cell proliferation rate is estimated by counting of initial and final cell numbers and shows normal cell growth. The results demonstrate the potential of ultrasonic standing waves as a tool for gentle and long-term manipulation of low cell numbers in microfluidic systems.
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  • Resultat 1-10 av 23

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