| 1. |
- Blom, Hans, et al.
(författare)
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STED microscopy : increased resolution for medical research?
- 2014
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Ingår i: Journal of Internal Medicine. - : Wiley. - 0954-6820 .- 1365-2796. ; 276:6, s. 560-578
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Forskningsöversikt (refereegranskat)abstract
- Optical imaging is crucial for addressing fundamental problems in all areas of life science. With the use of confocal and two-photon fluorescence microscopy, complex dynamic structures and functions in a plethora of tissue and cell types have been visualized. However, the resolution of classical' optical imaging methods is poor due to the diffraction limit and does not allow resolution of the cellular microcosmos. On the other hand, the novel stimulated emission depletion (STED) microscopy technique, because of its targeted on/off-switching of fluorescence, is not hampered by a diffraction-limited resolution barrier. STED microscopy can therefore provide much sharper images, permitting nanoscale visualization by sequential imaging of individual-labelled biomolecules, which should allow previous findings to be reinvestigated and provide novel information. The aim of this review is to highlight promising developments in and applications of STED microscopy and their impact on unresolved issues in biomedical science.
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| 2. |
- Fontana, Jacopo M., et al.
(författare)
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Calcium oscillations triggered by cardiotonic steroids
- 2013
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Ingår i: The FEBS Journal. - : Wiley. - 1742-464X .- 1742-4658. ; 280:21, s. 5450-5455
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Forskningsöversikt (refereegranskat)abstract
- Na+, K+-ATPase (NKA) is well known for its function as an ion pump. Studies during the last decade have revealed an additional role for NKA as a signal transducer. In this brief review, we describe how cardiotonic steroids, which are highly specific NKA ligands, trigger slow Ca2+ oscillations by promoting the interaction between NKA and the inositol trisphosphate receptor, and how this Ca2+ signal activates the NF-B subunit p65 and increases the expression of the antiapoptotic factor Bcl-xL. The potential tissue-protective effects of this signal are discussed.
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| 3. |
- Wiklund, Martin, et al.
(författare)
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Acoustofluidics 18 : Microscopy for acoustofluidic micro-devices
- 2012
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Ingår i: Lab on a Chip. - : Royal Society of Chemistry (RSC). - 1473-0197 .- 1473-0189. ; 12:18, s. 3221-3234
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Forskningsöversikt (refereegranskat)abstract
- In this tutorial review in the thematic series "Acoustofluidics", we discuss the implementation and practice of optical microscopy in acoustofluidic micro-devices. Examples are given from imaging of acoustophoretic manipulation of particles and cells in microfluidic channels, but most of the discussion is applicable to imaging in any lab-on-a-chip device. The discussion includes basic principles of optical microscopy, different microscopy modes and applications, and design criteria for micro-devices compatible with basic, as well as advanced, optical microscopy.
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