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Träfflista för sökning "WFRF:(Brookes Anthony J.) ;pers:(Russom Aman)"

Sökning: WFRF:(Brookes Anthony J.) > Russom Aman

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1.
  • Russom, Aman, et al. (författare)
  • Rapid melting curve analysis on monolayered beads for high-throughput genotyping of single-nucleotide polymorphisms
  • 2006
  • Ingår i: Analytical Chemistry. - : American Chemical Society (ACS). - 0003-2700 .- 1520-6882. ; 78:7, s. 2220-2225
  • Tidskriftsartikel (refereegranskat)abstract
    • This report describes a rapid solid-phase melting curve analysis method for single-nucleotide polymorphism (SNP) genotyping. The melting curve analysis is based on dynamic allele-specific hybridization (DASH). The DNA duplexes are conjugated on beads that are immobilized on the surface of a microheater chip with integrated heaters and temperature sensors. SNP on PCR products were scored, illustrating the sensitivity and robustness of the system. The method is based on random bead immobilization by microcontact printing. Single-bead detection and multiplexing were performed with a heating rate more than 20 times faster than conventional DASH. Analyses that took more than 15 min could be performed in less that 1 min, enabling ultrarapid SNP analysis. In addition, an array version of the chip was implemented enabling the preparation of an array of bead arrays for high-throughput and rapid SNP genotyping.
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2.
  • Russom, Aman, et al. (författare)
  • Ultra-rapid melting curve analysis on beads for high-throughput genotyping of single nucleotide polymorphism
  • 2005
  • Ingår i: Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference. - : Transducers Research Foundations. - 9780974361116 ; , s. 1006-1008
  • Konferensbidrag (refereegranskat)abstract
    • This report describes a rapid solid-phase melting curve analysis method based on dynamic allele-specific hybridization (DASH) for single nucleotide polymorphism (SNP) genotyping. Beads with DNA duplexes are immobilized on the surface of a microheater chip. SNP on PCR products were scored, illustrating the sensitivity and robustness of the system. Single-bead detection and multiplexing were performed with a heating rate more than 20 times faster than conventional DASH. Hence, analyses that took more than 15 minutes could be performed in less than 1 minute, enabling ultra-rapid SNP analysis.
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  • Resultat 1-2 av 2
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konferensbidrag (1)
tidskriftsartikel (1)
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refereegranskat (2)
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Andersson, Helene (2)
Stemme, Göran (2)
Haasl, Sjoerd (2)
Brookes, Anthony J. (2)
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Kungliga Tekniska Högskolan (2)
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Engelska (2)
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Medicin och hälsovetenskap (1)

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