| 1. |
- Beal, Jacob, et al.
(författare)
-
Robust estimation of bacterial cell count from optical density
- 2020
-
Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
-
Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
|
|
| 2. |
- Agrawal, Tarun, 1989, et al.
(författare)
-
Automating Loading and Unloading for Autonomous Transport: Identifying Challenges and Requirements with a Systems Approach
- 2023
-
Ingår i: IFIP Advances in Information and Communication Technology. - 1868-4238 .- 1868-422X. ; Part III, s. 332-345
-
Konferensbidrag (refereegranskat)abstract
- The logistics industry has undergone significant changes due to high demand, competition, cost pressures, interruptions, and labor market limitations affecting supply chains. As a result, there has been a significant adoption of automation in internal logistics such as warehousing, stock control, and material handling, leading to increased organizational competitiveness by reducing manual labor costs and time spent on these operations. The use of autonomous road transport holds potential to improve transport performance within areas of safety, sustainability, and efficiency. However, for autonomous transport to be fully ealized, loading and unloading processes at shipping and receiving facilities must also be automated. This paper takes a systems approach to identify the challenges and requirements for automated loading and unloading in a setting of autonomous truck transport potentially within a production setting. By addressing these challenges and meeting the necessary requirements, it may be possible to fully realize the benefits of autonomous transport and improve overall transport performance.
|
|
| 3. |
|
|
| 4. |
|
|
| 5. |
- Allen, J, et al.
(författare)
-
Sustainability Strategies for City Logistics
- 2012
-
Ingår i: McKinnon, A., Browne, M. and Whiteing, A. (eds.) Green Logistics: Improving the Environmental Sustainability of Logistics, Second Edition. - London : Kogan Page. - 9780749466251 ; , s. 289-311
-
Bokkapitel (refereegranskat)
|
|
| 6. |
- Allen, Julian, et al.
(författare)
-
Sustainability Strategies for City Logistics
- 2015
-
Ingår i: Green Logistics: Improving the Environmental Sustainability of Logistics, McKinnon, A., Browne, M., Whiteing, A. and Piecyk, M. (eds.) Third Edition. - London : Kogan Page. - 9780749471859
-
Bokkapitel (refereegranskat)
|
|
| 7. |
- Allen, J, et al.
(författare)
-
The role of urban consolidation centres in freight transport
- 2012
-
Ingår i: Evangelista, P., McKinnon, A., Sweeney, E. and Esposito, E. (eds) Supply Chain Innovation for Competing in Highly Dynamic Markets: Challenges and Solutions. - Hershey PA : Business Science Reference. - 9781609605858 ; , s. 199-214
-
Bokkapitel (refereegranskat)
|
|
| 8. |
- Ardeberg, Arne, et al.
(författare)
-
From Euro50 towards a European ELT
- 2006
-
Ingår i: Proceedings of SPIE - The International Society for Optical Engineering. - : SPIE. - 0277-786X .- 1996-756X. ; 6267 II, s. 26725-26725
-
Konferensbidrag (refereegranskat)abstract
- With Euro50 as a convenient telescope laboratory, the Euro50 team has continued development aiming at a European extremely large telescope (ELT). Here, we give a progress report. The needs of science and instrumentation are briefly discussed as is the importance of photometric stability and precision. Results are reported from work on integrated modelling. Details are given concerning point-spread functions (PSFs) obtained with and without adaptive optics (AO). Our results are rather encouraging concerning AO photometry and compensation of edge sensor noise as well as regarding seeing-limited ELT operation. The current status of our development of large deformable mirrors is shown. Low-cost actuators and deflection sensors have been developed as have hierarchic control algorithms. Fabrication of large thin mirror blanks as well as polishing and handling of thin mirrors has been studied experimentally. Regarding adaptive optics, we discuss differential refraction and the limitations imposed by dispersive optical path differences (OPDs) and dispersive anisoplanatism. We report on progress in laser guide star (LGS) performance and a real-time online experiment in multi-conjugate AO (MCAO). We discuss ELTs, high-resolution spectroscopy and pupil slicing with and without use of AO. Finally, we present some recent studies of ELT enclosure options.
|
|
| 9. |
- Arvidsson, Niklas, 1979, et al.
(författare)
-
A review of the success and failure of tram systems to carry urban freight: the implications for a low emission intermodal solution using electric vehicles on trams
- 2013
-
Ingår i: European Transport / Trasporti Europei. - 1825-3997. ; 54:5, s. 1825-3997
-
Tidskriftsartikel (refereegranskat)abstract
- This paper considers the potential use of trams and Electric Distribution Vehicles (EDVs) as cargo carriers in intermodal urban freight distribution. Distribution activities are vital for society but are also the cause of environmental and social problems. Transporting goods in urban areas, where most logistics chains start or end, is an activity that increasingly generates severe problems for all stakeholders, for instance, local authorities, the logistic industry, customers and society in general. New transport solutions are necessary in order to decrease traffic congestion, noise and traffic pollution, e.g., emissions of greenhouse gases and other pollutants in urban areas. A possible solution to these problems is to transform the current freight distribution system within cities, for example by favouring the enhancement of intermodal transport alternatives, i.e. combining road and rail transport. Information has been collected through a literature review and interviews in Amsterdam and from these results a conceptual model is presented, as well as a low emission concept using electric vehicles on trams in Gothenburg. The concept utilizes techniques from the shipping industry, train industry, and the car industry.
|
|
| 10. |
- Brettmo, Alena, et al.
(författare)
-
Business Improvement Districts as important influencers for changing to sustainable urban freight
- 2020
-
Ingår i: Cities. - : Elsevier BV. - 0264-2751. ; 97
-
Tidskriftsartikel (refereegranskat)abstract
- Abstract This paper explores the ways in which Business Improvement Districts (BIDs) can promote initiatives that lead to more sustainable urban goods movements in urban areas. The qualitative research was conducted by studying eight BIDs in three cities and in three countries - all the BIDs studied had considered or taken actions related to urban freight and sustainability. The results show that BIDs in different countries have similarities and differences in their organisational structure and functions, the main services provided and readiness to promote initiatives related to urban freight improvements. We identified that differences in their accomplishments relate to their motivation, awareness of the scope to influence urban freight and the extent of their collaboration with local and city authorities concerning urban freight initiatives. In general, we suggest that BIDs have the potential to act as important influencers in urban freight as they (i) unite the receivers and encourage them to address joint operations that bring economic and environmental benefits (ii) give the receivers the possibility to implement changes through the power of collective action (iii) facilitate the dialogue and collaboration with local and city authorities. As a result, we suggest that the measures directed to BIDs on improving urban freight can have much more significant return on time and funds invested than if invested in a single businesses and organisations. Our recommendation to BIDs that would like to reach higher level of accomplishments is to ensure stakeholder engagement including engaging and collaboration with local authorities and to demonstrate their willingness to take a lead.
|
|
