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1.
  • Klionsky, Daniel J., et al. (författare)
  • Guidelines for the use and interpretation of assays for monitoring autophagy
  • 2012
  • Ingår i: Autophagy. - : Landes Bioscience. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
  • Forskningsöversikt (refereegranskat)abstract
    • In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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6.
  • Kristanl, Matej, et al. (författare)
  • The Seventh Visual Object Tracking VOT2019 Challenge Results
  • 2019
  • Ingår i: 2019 IEEE/CVF INTERNATIONAL CONFERENCE ON COMPUTER VISION WORKSHOPS (ICCVW). - : IEEE COMPUTER SOC. - 9781728150239 ; , s. 2206-2241
  • Konferensbidrag (refereegranskat)abstract
    • The Visual Object Tracking challenge VOT2019 is the seventh annual tracker benchmarking activity organized by the VOT initiative. Results of 81 trackers are presented; many are state-of-the-art trackers published at major computer vision conferences or in journals in the recent years. The evaluation included the standard VOT and other popular methodologies for short-term tracking analysis as well as the standard VOT methodology for long-term tracking analysis. The VOT2019 challenge was composed of five challenges focusing on different tracking domains: (i) VOT-ST2019 challenge focused on short-term tracking in RGB, (ii) VOT-RT2019 challenge focused on "real-time" short-term tracking in RGB, (iii) VOT-LT2019 focused on long-term tracking namely coping with target disappearance and reappearance. Two new challenges have been introduced: (iv) VOT-RGBT2019 challenge focused on short-term tracking in RGB and thermal imagery and (v) VOT-RGBD2019 challenge focused on long-term tracking in RGB and depth imagery. The VOT-ST2019, VOT-RT2019 and VOT-LT2019 datasets were refreshed while new datasets were introduced for VOT-RGBT2019 and VOT-RGBD2019. The VOT toolkit has been updated to support both standard short-term, long-term tracking and tracking with multi-channel imagery. Performance of the tested trackers typically by far exceeds standard baselines. The source code for most of the trackers is publicly available from the VOT page. The dataset, the evaluation kit and the results are publicly available at the challenge website(1).
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7.
  • Ablikim, M., et al. (författare)
  • Observation of a Neutral Structure near the D(D)over-bar* Mass Threshold in e(+)e(-) -> (D(D)over-bar*)(0)pi(0) at root s=4.226 and 4.257 GeV
  • 2015
  • Ingår i: Physical Review Letters. - 0031-9007 .- 1079-7114. ; 115:22
  • Tidskriftsartikel (refereegranskat)abstract
    • A neutral structure in the D (D) over bar* system around the D (D) over bar* mass threshold is observed with a statistical significance greater than 10 sigma in the processes e(+)e(-) -> D+D*(-)pi(0) + c.c. and e(+)e(-) -> D-0(D) over bar*(0)pi(0) + c.c. at root s = 4.226 and 4.257 GeV in the BESIII experiment. The structure is denoted as Z(c)(3885)(0). Assuming the presence of a resonance, its pole mass and width are determined to be [3885.7(-5.7)(+4.3) (stat) +/- 8.4(syst)] MeV/c(2) and [35(-12)(+11) (stat) +/- 15(syst)] MeV, respectively. The Born cross sections are measured to be sigma[e(+)e(-) -> Z(c)(3885)(0)pi(0); Z(c)(3885)(0) -> D (D) over bar*] = [77 +/- 13(stat) +/- 17(syst)] pb at 4.226 GeV and [47 +/- 9(stat) +/- 10(syst)] pb at 4.257 GeV. The ratio of decay rates B[Z(c)(3885)(0) -> D+D*(-) + c.c.]/B[Z(c)(3885)(0) -> D-0(D) over bar*(0) + c.c.] is determined to be 0.96 +/- 0.18(stat) +/- 0.12(syst), consistent with no isospin violation in the process, Z(c)(3885)(0) -> D (D) over bar*.
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8.
  • Ablikim, M., et al. (författare)
  • Study of D+ -> K-pi(+)e(+)nu(e)
  • 2016
  • Ingår i: PHYSICAL REVIEW D. - 2470-0010. ; 94:3
  • Tidskriftsartikel (refereegranskat)abstract
    • We present an analysis of the decay D+ -> K-pi(+)e(+)nu(e) based on data collected by the BESIII experiment at the psi(3770) resonance. Using a nearly background-free sample of 18262 events, we measure the branching fraction B(D+ -> K-pi+e+nu e) = (3.77 +/- 0.03 +/- 0.08)%. For 0.8 < m(K pi) < 1.0 GeV/c(2), the partial branching fraction is B(D+ -> K-pi+e+nu e)([0.8,1.0]) = (3.39 +/- 0.03 +/- 0.08)%. A partial wave analysis shows that the dominant (K) over bar* (892)degrees component is accompanied by an S-wave contribution accounting for (6.05 +/- 0.22 +/- 0.18)% of the total rate and that other components are negligible. The parameters of the (K) over bar* (892)degrees resonance and of the form factors based on the spectroscopic pole dominance predictions are also measured. We also present a measurement of the (K) over bar* (892)degrees helicity basis form factors in a model-independent way.
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9.
  • Ablikim, M., et al. (författare)
  • Amplitude analysis of D0 -> K -π+π+π-
  • 2017
  • Ingår i: Physical Review D. - 2470-0010 .- 2470-0029. ; 95:7
  • Tidskriftsartikel (refereegranskat)abstract
    • We present an amplitude analysis of the decay D-0 -> K- pi(+)pi(+)pi(-) based on a data sample of 2.93 fb(-1) acquired by the BESIII detector at the psi(3770) resonance. With a nearly background free sample of about 16000 events, we investigate the substructure of the decay and determine the relative fractions and the phases among the different intermediate processes. Our amplitude model includes the two-body decays D-0 -> (K) over bar*(0)rho(0), D-0 -> K- a(1)(+) (1260) and D-0 -> K-1(-)(1270)pi(+), the three-body decays D-0 -> K-1(-)*(0)pi(+)pi(-) and D-0 -> K- pi(+)rho(0), as well as the four-body nonresonant decay D-0 -> K- pi(+)pi(+)pi(-). The dominant intermediate process is D-0 -> K(-)a(1)(+)(1260)accounting for a fit fraction of 54.6%.
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10.
  • Ablikim, M., et al. (författare)
  • Amplitude Analysis of the Decays eta ' -> pi(+)pi(-)pi(0) and eta' -> pi(0)pi(0)pi(0)
  • 2017
  • Ingår i: Physical Review Letters. - 0031-9007 .- 1079-7114. ; 118:1
  • Tidskriftsartikel (refereegranskat)abstract
    • Based on a sample of 1.31 x 10(9) J/Psi events collected with the BESIII detector, an amplitude analysis of the isospin-violating decays eta' -> pi(+)pi(-)pi(0) and eta' -> pi(0)pi(0)pi(0) is performed. A significant P-wave contribution from eta' -> rho(+/-)eta(-/+) is observed for the first time in eta' -> pi(+)pi(-)pi(0). The branching fraction is determined to be B(eta' -> rho(+/-)pi(-/+)) = (7.44 +/- 0.60 +/- 1.26 +/- 1.84) x 10(-4), where the first uncertainty is statistical, the second systematic, and the third model dependent. In addition to the nonresonant S-wave component, there is a significant sigma meson component. The branching fractions of the combined S-wave components are determined to be B(eta' -> pi(+)pi(-)pi(0))(S) = (37.63 +/- 0.77 +/- 2.22 +/- 4.48) x 10(-4) and B(eta' -> pi(0)pi(0)pi(0)) = (35.22 +/- 0.82 +/- 2.54) x 10(-4), respectively. The latter one is consistent with previous BESIII measurements.
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