| 1. |
- Markljung, Ellen, et al.
(författare)
-
ZBED6, a novel transcription factor derived from a domesticated DNA transposon regulates IGF2 expression and muscle growth
- 2009
-
Ingår i: PLoS biology. - : Public Library of Science (PLoS). - 1544-9173 .- 1545-7885. ; 7:12, s. e1000256-
-
Tidskriftsartikel (refereegranskat)abstract
- A single nucleotide substitution in intron 3 of IGF2 in pigs abrogates a binding site for a repressor and leads to a 3-fold up-regulation of IGF2 in skeletal muscle. The mutation has major effects on muscle growth, size of the heart, and fat deposition. Here, we have identified the repressor and find that the protein, named ZBED6, is previously unknown, specific for placental mammals, and derived from an exapted DNA transposon. Silencing of Zbed6 in mouse C2C12 myoblasts affected Igf2 expression, cell proliferation, wound healing, and myotube formation. Chromatin immunoprecipitation (ChIP) sequencing using C2C12 cells identified about 2,500 ZBED6 binding sites in the genome, and the deduced consensus motif gave a perfect match with the established binding site in Igf2. Genes associated with ZBED6 binding sites showed a highly significant enrichment for certain Gene Ontology classifications, including development and transcriptional regulation. The phenotypic effects in mutant pigs and ZBED6-silenced C2C12 myoblasts, the extreme sequence conservation, its nucleolar localization, the broad tissue distribution, and the many target genes with essential biological functions suggest that ZBED6 is an important transcription factor in placental mammals, affecting development, cell proliferation, and growth.
|
|
| 2. |
- Teghammar, Anna, 1981, et al.
(författare)
-
Improved Anaerobic Digestion by the Addition of Paper Tube Residuals: Pretreatment, Stabilizing, and Synergetic Effects
- 2013
-
Ingår i: Energy & Fuels. - : American Chemical Society (ACS). - 1520-5029 .- 0887-0624. ; 27:1, s. 277-284
-
Tidskriftsartikel (refereegranskat)abstract
- This study deals with the addition of paper tube residuals to a nitrogen-rich mixture of organic waste obtained from industrial and municipal activities. This nitrogen-rich mixture, called buffer tank substrate (BTS) in the following text, is used in a large-scale biogas plant. The effects were investigated in semi-continuous co-digestion processes, and variations in operational conditions were studied. The addition of paper tubes had stabilizing effects, prevented the failure of the process, and made it possible to decrease the hydraulic retention time from 25 to 20 days. Furthermore, synergetic effects were found, with 15-34% higher methane yields, when paper tubes were co-digested with BTS. Moreover, steam explosion pretreatment of the paper tube waste with the addition of 0-2% NaOH was evaluated by batch digestion experiments. Increasing the NaOH concentrations used in the pretreatment resulted in increasing methane yields, with the highest of 403 N mL of CH4 g(-1) of volatile solids (VS) corresponding to an increase by 50% compared to that when untreated paper was digested (268 N mL of CH4 g(-1) of VS). The long-term effects of this best pretreatment were further investigated by continuous co-digestion experiments, leading to a higher methane yield when pretreated paper tubes were used in the co-digestion process compared to untreated.
|
|
| 3. |
- Marhendraswari, M. B.D., et al.
(författare)
-
Production of edible fungal (Rhizopus delemar CBS 145940) biomass from organosolv-pretreated oil palm empty fruit bunch (OPEFB) in submerged fermentation
- 2020
-
Ingår i: IOP Conference Series: Materials Science and Engineering. - : Institute of Physics (IOP). - 1757-8981 .- 1757-899X. ; 991:1
-
Konferensbidrag (refereegranskat)abstract
- Accumulation of oil palm empty fruit bunches (OPEFB) from palm oil industry poses challenges for the disposal process, which leads to environmental damage. For this reason, valorization of OPEFB fractions to produce edible fungal biomass was carried out in this research. The fungus was Rhizopus delemar CBS 145940, which is an edible fungus, Indonesian indigenous, and is favorable for the production of several end products. Organosolv pretreatment was first conducted on OPEFB using ethanol (50%) as the solvent. Enzymatic hydrolysis was then performed using Cellic® Ctec3 on the pretreated-OPEFB fractions. Hydrolyzates from cellulose-rich fraction, slurry (a mixture of cellulose-rich fraction and hemicellulose-rich fraction), and hemicellulose-rich fraction were used as the cultivation media for fungal growth. The corresponding yield of fungal biomass from each medium was 0.62 ± 0.07 g/g glucose; 0.41 ± 0.02; and 0.61 ± 0.13 g/g fermentable sugars, respectively. These results showed that Rhizopus delemar CBS 145940 could be grown in all the hydrolyzates from the OPEFB fractions. Nevertheless, in order to obtain higher fungal biomass, supplementation of nutrition was needed.
|
|
