| 1. |
- Blösch, Günter, et al.
(författare)
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Twenty-three unsolved problems in hydrology (UPH) - a community perspective
- 2019
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Ingår i: Hydrological Sciences Journal. - : Informa UK Limited. - 0262-6667 .- 2150-3435. ; 64:10, s. 1141-1158
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Tidskriftsartikel (refereegranskat)abstract
- This paper is the outcome of a community initiative to identify major unsolved scientific problems in hydrology motivated by a need for stronger harmonisation of research efforts. The procedure involved a public consultation through online media, followed by two workshops through which a large number of potential science questions were collated, prioritised, and synthesised. In spite of the diversity of the participants (230 scientists in total), the process revealed much about community priorities and the state of our science: a preference for continuity in research questions rather than radical departures or redirections from past and current work. Questions remain focused on the process-based understanding of hydrological variability and causality at all space and time scales. Increased attention to environmental change drives a new emphasis on understanding how change propagates across interfaces within the hydrological system and across disciplinary boundaries. In particular, the expansion of the human footprint raises a new set of questions related to human interactions with nature and water cycle feedbacks in the context of complex water management problems. We hope that this reflection and synthesis of the 23 unsolved problems in hydrology will help guide research efforts for some years to come.
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| 2. |
- Klionsky, Daniel J., et al.
(författare)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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| 3. |
- Birney, Ewan, et al.
(författare)
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Identification and analysis of functional elements in 1% of the human genome by the ENCODE pilot project
- 2007
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Ingår i: Nature. - : Springer Science and Business Media LLC. - 0028-0836 .- 1476-4687. ; 447:7146, s. 799-816
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Tidskriftsartikel (refereegranskat)abstract
- We report the generation and analysis of functional data from multiple, diverse experiments performed on a targeted 1% of the human genome as part of the pilot phase of the ENCODE Project. These data have been further integrated and augmented by a number of evolutionary and computational analyses. Together, our results advance the collective knowledge about human genome function in several major areas. First, our studies provide convincing evidence that the genome is pervasively transcribed, such that the majority of its bases can be found in primary transcripts, including non-protein-coding transcripts, and those that extensively overlap one another. Second, systematic examination of transcriptional regulation has yielded new understanding about transcription start sites, including their relationship to specific regulatory sequences and features of chromatin accessibility and histone modification. Third, a more sophisticated view of chromatin structure has emerged, including its inter-relationship with DNA replication and transcriptional regulation. Finally, integration of these new sources of information, in particular with respect to mammalian evolution based on inter- and intra-species sequence comparisons, has yielded new mechanistic and evolutionary insights concerning the functional landscape of the human genome. Together, these studies are defining a path for pursuit of a more comprehensive characterization of human genome function.
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| 4. |
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| 5. |
- Clark, Eric, et al.
(författare)
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Foreword
- 2009
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Ingår i: National Geographic countries of the world. - 9781426303890 ; , s. 4-5
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Bokkapitel (populärvet., debatt m.m.)
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| 6. |
- Clark, Eric, et al.
(författare)
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Grepp på stadskrigsbilismen
- 2003
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Ingår i: Tidrumsfragment : en vänbok till Bo Lenntorp. - 9197444839
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
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| 7. |
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| 8. |
- Aad, G., et al.
(författare)
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- 2011
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swepub:Mat__t (refereegranskat)
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| 9. |
- Clark, Eric, et al.
(författare)
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Islands: ecologically unequal exchange and landesque capital
- 2012
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Ingår i: Ecology and power: struggles over land and material resources in the past, present and future. - 9780415601467 ; , s. 52-67
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Bokkapitel (refereegranskat)abstract
- Our purpose in this chapter is to give a brief perspective on islands in global historical-political ecology, focusing on ecologically unequal exchange and the formation of landesque capital. We emphasize how these processes are intertwined and recursively connected in broader contexts of uneven development. Empirical examples draw on our research into the historical-political ecology of islands and archipelagos which have since 1949 been geopolitically part of Taiwan, the Republic of China: Kinmen (Quemoy) Island in the Fukien Province of China, the Penghu Archipelago (Pescadores) in the Taiwan Strait, and Pongso no Tau (Lanyu, Orchid Island), a northern outlier of the Batanes Islands, southeast of Taiwan. The aim is not so much to draw comparisons between these cases as to draw connections, both between the processes of ecologically unequal exchange and formation of landesque capital, and between the peripheral islands and the global political economy/ecology of which they are part.
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| 10. |
- Clark, Eric, et al.
(författare)
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Isolating Connections - Connecting Isolations
- 2009
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Ingår i: Geografiska Annaler. Series B. Human Geography. - 1468-0467. ; 91B:4, s. 311-323
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Forskningsöversikt (refereegranskat)abstract
- The varied and distinct ways we connect can facilitate or impose isolation, our own or someone else's. Different forms of isolation are themselves interconnected and sometimes enrich our connecting. The relation between isolation and connection, we argue, is one of complementarity, like Calvino's 'two inseparable and complementary functions of life ... syntony, or participation in the world around us ... [and] focalization or constructive concentration.' Solitude sought can enhance connections. Imposed isolation weakens connections in ways both obvious and subtle. This contrast between sought and imposed underscores the influence of hierarchy and socially produced inequities, excesses of which fragment the social ties that could constrain or diminish these same inequities. Deep inequity degrades the quality of both connections and isolation, at significant costs to our health, ecology, economy, cultural diversity, and political vitality. From this vantage point, we cull ways to improve our syntony and our focalization, fulfilling by expressing those shared egalitarian moral sentiments that motivate connections of solidarity partly in the interest of being "left alone".
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